Small Angle Scattering Biological Data Bank SASBDB

SASDB69 – E244K monomeric human Properdin

E244K Human Properdin experimental SAS data

Sample:	E244K Human Properdin monomer, 49 kDa Homo sapiens protein
Buffer:	10 mM HEPES 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jul 27

Functional and structural insight into properdin control of complement alternative pathway amplification. EMBO J 36(8):1084-1099 (2017)
Pedersen DV, Roumenina L, Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR

R_g^Guinier	4.1	nm
D_max	15.0	nm

SASDBE7 – ESX-5 type VII secretion system protein EccC5

ESX-5 type VII secretion system protein EccC5 experimental SAS data

ESX-5 type VII secretion system protein EccC5 Rg histogram

Sample:	ESX-5 type VII secretion system protein EccC5 monomer, 142 kDa Mycobacterium xenopi RIVM700367 protein
Buffer:	20 mM Tris 200 mM NaCl 5%(v/v) Glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jul 15

Structure of the mycobacterial ESX-5 type VII secretion system membrane complex by single-particle analysis. Nat Microbiol 2:17047 (2017)
Beckham KS, Ciccarelli L, Bunduc CM, Mertens HD, Ummels R, Lugmayr W, Mayr J, Rettel M, Savitski MM, Svergun DI, Bitter W, Wilmanns M, Marlovits TC, Parret AH, Houben EN

R_g^Guinier	6.2	nm
D_max	23.0	nm
Volume^Porod	255	nm³

SASDBS6 – Apo form of full length ObgE from E.coli (ObgE_FL)

Apo form of full length ObgE from E.coli (ObgE_FL) Rg histogram

Sample:	GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer:	20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 17

Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W

R_g^Guinier	3.7	nm
D_max	18.1	nm
Volume^Porod	102	nm³

SASDBT6 – GppNHp bound form of full length ObgE from E.coli (ObgE_FL with GppNHp)

Sample:	GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 17

R_g^Guinier	3.7	nm
D_max	14.5	nm
Volume^Porod	90	nm³

SASDBU6 – Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340)

Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340) Rg histogram

Sample:	GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer:	20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Mar 12

R_g^Guinier	3.1	nm
D_max	11.6	nm
Volume^Porod	70	nm³

SASDBV6 – GppNHp bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GppNHp)

Sample:	GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Mar 12

R_g^Guinier	3.1	nm
D_max	11.4	nm
Volume^Porod	72	nm³

SASDBA9 – E. coli CcdB2-CcdA2-CcdB2 toxin/antitoxin heterohexamer complex

Toxin CcdBAntitoxin CcdAToxin CcdB experimental SAS data

Sample:	Toxin CcdB dimer, 23 kDa Escherichia coli protein Antitoxin CcdA dimer, 17 kDa Escherichia coli protein Toxin CcdB dimer, 23 kDa Escherichia coli protein
Buffer:	10 mM Tris 50 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Jul 24

Molecular mechanism governing ratio-dependent transcription regulation in the ccdAB operon. Nucleic Acids Res 45(6):2937-2950 (2017)
Vandervelde A, Drobnak I, Hadži S, Sterckx YG, Welte T, De Greve H, Charlier D, Efremov R, Loris R, Lah J

R_g^Guinier	3.4	nm
D_max	12.0	nm
Volume^Porod	92	nm³

SASDBB9 – GDP bound form of full length ObgE from E.coli (ObgE_FL with GDP)

Sample:	GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 17

R_g^Guinier	3.5	nm
D_max	16.0	nm
Volume^Porod	94673	nm³

SASDBC9 – GDP bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GDP)

Sample:	GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5
Experiment:	SAXS data collected at Rigaku BioSAXS-2000, on 2015 Feb 1

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	70673	nm³

SASDJB2 – Restriction endonuclease R.AgeI in apo form, monomeric

Type-2 restriction enzyme AgeI experimental SAS data

Sample:	Type-2 restriction enzyme AgeI monomer, 31 kDa Thalassobius gelatinovorus protein
Buffer:	10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl₂, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 14

Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA. Nucleic Acids Res 45(6):3547-3558 (2017)
Tamulaitiene G, Jovaisaite V, Tamulaitis G, Songailiene I, Manakova E, Zaremba M, Grazulis S, Xu SY, Siksnys V

R_g^Guinier	2.2	nm
D_max	6.7	nm
Volume^Porod	53	nm³

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