Small Angle Scattering Biological Data Bank SASBDB

SASDBU6 – Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340)

Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340) Rg histogram

Sample:	GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer:	20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Mar 12

Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence. J Biol Chem 292(14):5871-5883 (2017)
Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W

R_g^Guinier	3.1	nm
D_max	11.6	nm
Volume^Porod	70	nm³

SASDBV6 – GppNHp bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GppNHp)

Sample:	GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Mar 12

R_g^Guinier	3.1	nm
D_max	11.4	nm
Volume^Porod	72	nm³

SASDBA9 – E. coli CcdB2-CcdA2-CcdB2 toxin/antitoxin heterohexamer complex

Toxin CcdBAntitoxin CcdAToxin CcdB experimental SAS data

Sample:	Toxin CcdB dimer, 23 kDa Escherichia coli protein Antitoxin CcdA dimer, 17 kDa Escherichia coli protein Toxin CcdB dimer, 23 kDa Escherichia coli protein
Buffer:	10 mM Tris 50 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Jul 24

Molecular mechanism governing ratio-dependent transcription regulation in the ccdAB operon. Nucleic Acids Res 45(6):2937-2950 (2017)
Vandervelde A, Drobnak I, Hadži S, Sterckx YG, Welte T, De Greve H, Charlier D, Efremov R, Loris R, Lah J

R_g^Guinier	3.4	nm
D_max	12.0	nm
Volume^Porod	92	nm³

SASDBB9 – GDP bound form of full length ObgE from E.coli (ObgE_FL with GDP)

Sample:	GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 17

R_g^Guinier	3.5	nm
D_max	16.0	nm
Volume^Porod	94673	nm³

SASDBC9 – GDP bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GDP)

Sample:	GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein
Buffer:	20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5
Experiment:	SAXS data collected at Rigaku BioSAXS-2000, on 2015 Feb 1

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	70673	nm³

SASDJB2 – Restriction endonuclease R.AgeI in apo form, monomeric

Type-2 restriction enzyme AgeI experimental SAS data

Sample:	Type-2 restriction enzyme AgeI monomer, 31 kDa Thalassobius gelatinovorus protein
Buffer:	10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl₂, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 14

Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA. Nucleic Acids Res 45(6):3547-3558 (2017)
Tamulaitiene G, Jovaisaite V, Tamulaitis G, Songailiene I, Manakova E, Zaremba M, Grazulis S, Xu SY, Siksnys V

R_g^Guinier	2.2	nm
D_max	6.7	nm
Volume^Porod	53	nm³

SASDJC2 – Restriction endonuclease R.AgeI complex with cognate DNA

Type-2 restriction enzyme AgeICognate DNA oligoduplex with 5'-T overhang experimental SAS data

Sample:	Type-2 restriction enzyme AgeI dimer, 61 kDa Thalassobius gelatinovorus protein Cognate DNA oligoduplex with 5'-T overhang dimer, 8 kDa DNA
Buffer:	10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl₂, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 14

R_g^Guinier	2.4	nm
D_max	7.4	nm
Volume^Porod	69	nm³

SASDB99 – Extracellular assembly of the human TSLP:TSLPR:IL-7Ralpha complex

Thymic stromal lymphopoietinInterleukin-7 receptor subunit alphaCytokine receptor-like factor 2 experimental SAS data

Sample:	Thymic stromal lymphopoietin monomer, 15 kDa Homo sapiens protein Interleukin-7 receptor subunit alpha monomer, 26 kDa Homo sapiens protein Cytokine receptor-like factor 2 monomer, 24 kDa Homo sapiens protein
Buffer:	10 mM Hepes, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Apr 8

Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma. Nat Commun 8:14937 (2017)
Verstraete K, Peelman F, Braun H, Lopez J, Van Rompaey D, Dansercoer A, Vandenberghe I, Pauwels K, Tavernier J, Lambrecht BN, Hammad H, De Winter H, Beyaert R, Lippens G, Savvides SN

R_g^Guinier	3.1	nm
D_max	10.3	nm
Volume^Porod	98	nm³

SASDBD3 – Leishmania braziliensis heat shock protein 90 (Hsp90).

Leishmania braziliensis heat shock protein 90 (Hsp90) experimental SAS data

Sample:	Leishmania braziliensis heat shock protein 90 (Hsp90) dimer, 166 kDa Leishmania braziliensis protein
Buffer:	25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2011 Sep 1

Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering. Int J Biol Macromol 97:503-512 (2017)
Seraphim TV, Silva KP, Dores-Silva PR, Barbosa LR, Borges JC

R_g^Guinier	5.3	nm
D_max	21.0	nm
Volume^Porod	380	nm³

SASDBE3 – Leishmania braziliensis heat shock protein 90 (Hsp90) N domain.

Leishmania braziliensis heat shock protein 90 (Hsp90) N domain experimental SAS data

Sample:	Leishmania braziliensis heat shock protein 90 (Hsp90) N domain monomer, 26 kDa Leishmania braziliensis protein
Buffer:	25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2011 Sep 1

R_g^Guinier	2.0	nm
D_max	7.5	nm
Volume^Porod	40	nm³

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