Glucosidase/Rutinosidase from Mucor circinelloides, Penicillium chrysogenum and Aspergillus niger

Jan Stransky

Submitted to SASBDB: 2024 Oct 24 Published in SASBDB: 2026 Oct 24

SASDVL8 – Glycosidase/Rutinosidase from Mucor circinelloides - deglycosylated monomer Sample: Glucan 1,3-beta-glucosidase monomer, 45 kDa Mucor circinelloides protein Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.1 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2020 Mar 26 RgGuinier 2.3 nm Dmax 6.4 nm VolumePorod 53 nm3

SASDVM8 – Glycosidase/Rutinosidase from Mucor circinelloides - deglycosylated dimer Sample: Glucan 1,3-beta-glucosidase dimer, 92 kDa Mucor circinelloides protein Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jun 27 RgGuinier 4.1 nm Dmax 14.4 nm VolumePorod 140 nm3

SASDVN8 – Glycosidase/Rutinosidase from Mucor circinelloides - glycosylated dimer Sample: Glucan 1,3-beta-glucosidase (short form, glycosylated) dimer, 89 kDa Mucor circinelloides protein Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jun 28 RgGuinier 3.3 nm Dmax 11.0 nm VolumePorod 103 nm3

SASDVP8 – Glycosidase/Rutinosidase from Mucor circinelloides - glycosylated tetramer Sample: Glucan 1,3-beta-glucosidase (long form, glycosylated) tetramer, 184 kDa Mucor circinelloides protein Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Jun 28 RgGuinier 4.8 nm Dmax 15.1 nm VolumePorod 171 nm3

SASDVQ8 – Glucosidase/Rutinosidase from Penicillium rubens - deglycosylated Sample: Glucan 1,3-beta-glucosidase monomer, 42 kDa Penicillium rubens (strain … protein Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2019 Nov 8 RgGuinier 2.1 nm Dmax 5.5 nm VolumePorod 65 nm3

SASDVR8 – Glucosidase/Rutinosidase from Penicillium rubens - glycosylated Sample: Glucan 1,3-beta-glucosidase monomer, 42 kDa Penicillium rubens (strain … protein Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2019 Nov 8 RgGuinier 2.2 nm Dmax 7.6 nm VolumePorod 68 nm3

SASDVS8 – Glucosidase/Rutinosidase from Aspergillus niger - deglycosylated Sample: Glucan 1,3-beta-glucosidase (W90L, N179H) monomer, 42 kDa Aspergillus niger (strain … protein Buffer: McIlvain (Na2HPO4/citric acid) buffer, pH: 3.5 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2021 Jun 8 RgGuinier 2.2 nm Dmax 6.1 nm VolumePorod 55 nm3

SASDVT8 – Glucosidase/Rutinosidase from Aspergillus niger - glycosylated Sample: Glucan 1,3-beta-glucosidase (W90L, N179H) dimer, 83 kDa Aspergillus niger (strain … protein Buffer: McIlvain (Na2HPO4/citric acid) buffer, pH: 3.5 Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2021 Jun 8 RgGuinier 3.0 nm Dmax 8.1 nm VolumePorod 111 nm3