Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release.

Leloup N, Lössl P, Meijer DH, Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
Nat Commun 8(1):1708 (2017 Nov 22)

PMID: 29167428 doi: 10.1038/s41467-017-01485-5

Submitted to SASBDB: 2017 Jul 8 Published in SASBDB: 2017 Dec 1

SASDCW5 – Dimeric Sortilin at pH 7.4 Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17 RgGuinier 3.9 nm Dmax 11.0 nm VolumePorod 275 nm3

SASDCX5 – Monomeric Sortilin at pH 5.5 Sample: Sortilin 1 A464E alias Neurotensin-receptor 3 A464E monomer, 76 kDa Mus musculus protein Buffer: 25 mM MES pH 5.5, 150 mM NaCl, pH: 5.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17 RgGuinier 3.4 nm Dmax 10.0 nm VolumePorod 217 nm3

SASDCY5 – Dimeric Sortilin at pH 5.5 Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein Buffer: 25 mM MES pH 5.5, 150 mM NaCl, pH: 5.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17 RgGuinier 3.9 nm Dmax 11.0 nm VolumePorod 336 nm3

SASDCZ5 – Monomeric Sortilin at pH 7.4 Sample: Sortilin 1 A464E alias Neurotensin-receptor 3 A464E monomer, 76 kDa Mus musculus protein Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17 RgGuinier 3.3 nm Dmax 10.5 nm VolumePorod 217 nm3

SASDCE7 – Monomeric Sortilin at pH 7.4 in the presence of neurotensin Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17 RgGuinier 3.3 nm Dmax 11.7 nm VolumePorod 192 nm3

SASDCF7 – Dimeric Sortilin at pH 7.4 in the presence of neurotensin Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17 RgGuinier 3.7 nm Dmax 13.5 nm VolumePorod 253 nm3