Small Angle Scattering Biological Data Bank SASBDB


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20 hits found for Guerra

SASDX42 – IgA peptidase (A31-I1166; active site mutant E540A)

IgA protease (E540A) experimental SAS data

IgA peptidase (A31-I1166; active site mutant E540A) Rg histogram

Sample:	IgA protease (E540A) monomer, 128 kDa Thomasclavelia ramosa protein
Buffer:	50 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 May 23

Biochemical and structural characterization of the human gut microbiome metallopeptidase IgAse provides insight into its unique specificity for the Fab' region of IgA1 and IgA2. PLoS Pathog 21(7):e1013292 (2025)
...Guerra P, Eckhard U, Gomis-Rüth FX

R_g^Guinier	5.7	nm
D_max	27.9	nm
Volume^Porod	190	nm³

SASDQT4 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
Guerra JPL, Blanchet CE, Vieira BJC, Waerenborgh JC, Jones NC, Hoffmann SV, Pereira AS, Tavares P

R_g^Guinier	4.5	nm
D_max	20.0	nm
Volume^Porod	454	nm³

SASDQU4 – DNA-binding protein from starved cells: DgrDpsWT + 6 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	18.4	nm
Volume^Porod	440	nm³

SASDQV4 – DNA-binding protein from starved cells: DgrDpsWT + 12 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	15.5	nm
Volume^Porod	464	nm³

SASDQW4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	14.1	nm
Volume^Porod	439	nm³

SASDQX4 – DNA-binding protein from starved cells: DgrDpsWT + 48 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	14.4	nm
Volume^Porod	427	nm³

SASDQY4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl, 5 mM EDTA

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS, 230 mM NaCl, 5 mM EDTA, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	19.5	nm
Volume^Porod	453	nm³

SASDQZ4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Co2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	13.9	nm
Volume^Porod	458	nm³

SASDQ25 – DNA-binding protein from starved cells: DgrDpsWT + 24 Mn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	19.3	nm
Volume^Porod	438	nm³

SASDQ35 – DNA-binding protein from starved cells: DgrDps-D43A mutant in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation experimental SAS data

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	19.8	nm
Volume^Porod	451	nm³

SASDQ45 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	19.1	nm
Volume^Porod	412	nm³

SASDQ55 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 48 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	20.7	nm
Volume^Porod	409	nm³

SASDQ65 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Co2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	20.6	nm
Volume^Porod	404	nm³

SASDQ75 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Mn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	19.5	nm
Volume^Porod	431	nm³

SASDNY7 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 50 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength International Journal of Molecular Sciences 23(9):4871 (2022)
Guerra J, Blanchet C, Vieira B, Almeida A, Waerenborgh J, Jones N, Hoffmann S, Tavares P, Pereira A

R_g^Guinier	4.4	nm
D_max	16.1	nm
Volume^Porod	409	nm³

SASDNZ7 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 80 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 80 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	4.5	nm
D_max	17.1	nm
Volume^Porod	475	nm³

SASDN28 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 230 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	4.5	nm
D_max	20.6	nm
Volume^Porod	438	nm³

SASDN38 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 480 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 480 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	4.8	nm
D_max	20.5	nm
Volume^Porod	430	nm³

SASDN48 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 50 mM NaCl pH 7.0

DNA protection during starvation, DPS-∆N (Ferritin superfamily) experimental SAS data

Sample:	DNA protection during starvation, DPS-∆N (Ferritin superfamily) dodecamer, 218 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	3.8	nm
D_max	9.3	nm
Volume^Porod	290	nm³

SASDN58 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 230 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS-∆N (Ferritin superfamily) dodecamer, 218 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	3.8	nm
D_max	8.8	nm
Volume^Porod	291	nm³

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