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38 hits found for Lund

SASDVZ9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 66 % D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetic acid, 66% v/v D₂O, pH: 3.2
Experiment: SANS data collected at D11, ILL on 2019 Jul 5
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDF22 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.

somapacitanFcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagneonatal Fc receptor ectodomain alpha-chain experimental SAS data
somapacitan FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: somapacitan monomer, 22 kDa Homo sapiens protein
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 May 11
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 4.2 nm
Dmax 14.7 nm
VolumePorod 227 nm3

SASDW22 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 80 % D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetic acid, 80% v/v D₂O, pH: 3.2
Experiment: SANS data collected at D11, ILL on 2019 Jul 5
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDF32 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the albumin-binding side chain of the human growth hormone derivative somapacitan.

FcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagAlbumin-binding side-chainneonatal Fc receptor ectodomain alpha-chain experimental SAS data
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag Albumin-binding side-chain neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
Albumin-binding side-chain monomer, 1 kDa
neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Nov 11
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 3.6 nm
Dmax 12.6 nm
VolumePorod 174 nm3

SASDW32 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 100% D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetic acid, 100% v/v D₂O, pH: 3.2
Experiment: SANS data collected at D11, ILL on 2019 Jul 5
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDW42 – 3 mg/ml β-chitin nanofibers from squid pens, SANS in 100 % D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetate, 99.7% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDW52 – 3 mg/ml β-chitin nanofibers from squid pens, SANS in 47 % D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2019 Sep 21
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDW72 – Non-deuterated N-acetylglucosamine binding Protein A (H-GbpA) in 47 % D₂O, 2.5 mg/mL

GlcNAc-binding protein A experimental SAS data
GlcNAc-binding protein A Kratky plot
Sample: GlcNAc-binding protein A monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 100 mM NaCl, 20 mM Tris, 47% v/v D₂O, pH: 8
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen
RgGuinier 25.0 nm

SASDW82 – Perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O, 2.4 mg/mL

GlcNAc-binding protein A (perdeuterated) experimental SAS data
GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 100 mM NaCl, 20 mM Tris, 47% v/v D₂O, pH: 8
Experiment: SANS data collected at D11, ILL on 2019 Sep 21
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen
RgGuinier 3.7 nm
Dmax 12.7 nm

SASDW92 – 10 mg/mL β-chitin nanofibers from squid pens with 2.5 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWA2 – 3 mg/mL β-chitin nanofibers from squid pens with 2.5 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWB2 – 3 mg/mL β-chitin nanofibers from squid pens with 2.5 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O - USANS

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at BT5 USANS, NIST Center for Neutron Research on 2020 Dec 4
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWC2 – 2.4 mg/mL β-chitin nanofibers from squid pens with 2 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2019 Sep 21
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWD2 – 2.4 mg/mL β-chitin nanofibers from squid pens with 0.5 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2019 Sep 21
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWE2 – 1.2 mg/mL β-chitin nanofibers from squid pens with 1 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 72 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Acetate, 72% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2019 Sep 21
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWF2 – 0.48 mg/mL β-chitin nanofibers from squid pens with 1.2 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2019 Sep 21
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWG2 – 3 mg/mL β-chitin nanofibers from squid pens with 2.5 mg/mL non-deuterated N-acetylglucosamine binding Protein A (H-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWH2 – 3 mg/mL β-chitin nanofibers from squid pens with a mixture of 1.25 mg/mL perdeuterated and 1.25 mg/mL non-deuterated N-acetylglucosamine binding Protein A (D-GbpA/H-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated)GlcNAc-binding protein A experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) GlcNAc-binding protein A Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
GlcNAc-binding protein A monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWJ2 – 3 mg/mL β-chitin nanofibers from squid pens with a mixture of 0.625 mg/mL perdeuterated and 1.875 mg/mL non-deuterated N-acetylglucosamine binding Protein A (D-GbpA/H-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated)GlcNAc-binding protein A experimental SAS data
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) GlcNAc-binding protein A Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
GlcNAc-binding protein A monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDEM3 – Dimeric class D beta-lactamase OXA-48

Beta-lactamase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Beta-lactamase dimer, 56 kDa Klebsiella pneumoniae protein
Buffer: 50 mM HEPES 50 mM K2SO4, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 23
The biological assembly of OXA-48 reveals a dimer interface with high charge complementarity and very high affinity. FEBS J (2018)
Lund BA, Thomassen AM, Nesheim BHB, Carlsen TJO, Isaksson J, Christopeit T, Leiros HS
RgGuinier 2.5 nm
Dmax 7.4 nm
VolumePorod 74 nm3

SASDJY3 – Immunoglobulin HUI-018 Fab in complex with human insulin

Insulin (Insulin B chain and Insulin A chain)HUI-018 Fab experimental SAS data
OTHER model
Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein
HUI-018 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 3.4 nm
Dmax 10.7 nm
VolumePorod 86 nm3

SASDJZ3 – Immunoglobulin HUI-018 Fab in complex with human insulin at high concentration

Insulin (Insulin B chain and Insulin A chain)HUI-018 Fab experimental SAS data
OTHER model
Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein
HUI-018 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 3.7 nm
Dmax 13.4 nm
VolumePorod 105 nm3

SASDQR8 – Exon1 of Non pathogenic form of Huntingtin (H16) with GFP fused at the C-terminus

Huntingtin experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Huntingtin monomer, 39 kDa Homo sapiens protein
Buffer: 20mM BisTris-HCl, 150mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 3
The structure of pathogenic huntingtin exon 1 defines the bases of its aggregation propensity. Nat Struct Mol Biol (2023)
...Lund XL, Shi ZD, Costa L, Thureau A, Allemand F, Swenson RE, Milhiet PE, Crehuet R, Barducci A, Cortés J, Sinnaeve D, Sibille N, Bernadó P
RgGuinier 3.3 nm
Dmax 16.0 nm
VolumePorod 63 nm3

SASDQS8 – Exon1 of Pathogenic form of Huntingtin (H46) with GFP fused at the C-terminus

Huntingtin experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Huntingtin monomer, 43 kDa Homo sapiens protein
Buffer: 20mM BisTris-HCl, 150mM NaCl, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 28
The structure of pathogenic huntingtin exon 1 defines the bases of its aggregation propensity. Nat Struct Mol Biol (2023)
...Lund XL, Shi ZD, Costa L, Thureau A, Allemand F, Swenson RE, Milhiet PE, Crehuet R, Barducci A, Cortés J, Sinnaeve D, Sibille N, Bernadó P
RgGuinier 4.2 nm

SASDCV8 – Uncharacterized protein CTHT_0072540 (Prp19 full-length) from Chaetomium thermophilum

Full-length hypothetical protein CTHT_0072540 experimental SAS data
SASREF model
Sample: Full-length hypothetical protein CTHT_0072540 tetramer, 207 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Oct 15
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
RgGuinier 6.2 nm
Dmax 23.0 nm
VolumePorod 280 nm3

SASDS29 – The DNA binding and transactivation domains (DBD-TAD) of Hepatocyte nuclear factor 1-alpha (HNF-1A)

Hepatocyte nuclear factor 1-alpha experimental SAS data
Hepatocyte nuclear factor 1-alpha Kratky plot
Sample: Hepatocyte nuclear factor 1-alpha monomer, 62 kDa Homo sapiens protein
Buffer: 4 mM Tris, 100 mM NaCl, 1 mM TCEP,, pH: 8.5
Experiment: SAXS data collected at CoSAXS, MAX IV on 2022 Apr 2
Structural properties of the HNF-1A transactivation domain Frontiers in Molecular Biosciences 10 (2023)
Kind L, Driver M, Raasakka A, Onck P, Njølstad P, Arnesen T, Kursula P
RgGuinier 4.9 nm
Dmax 17.0 nm
VolumePorod 109 nm3

SASDVV9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in H₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetic acid, pH: 3.2
Experiment: SANS data collected at D11, ILL on 2019 Jul 5
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDVW9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 20 % D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetic acid, 20% v/v D₂O, pH: 3.2
Experiment: SANS data collected at D11, ILL on 2019 Jul 5
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDVX9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 42 % D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetic acid, 42% v/v D₂O, pH: 3.2
Experiment: SANS data collected at D11, ILL on 2019 Jul 5
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDVY9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 47 % D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDK42 – Chitin-binding protein CbpD (Pseudomonas aeruginosa), ESRF BM29 data

Chitin-binding protein CbpD experimental SAS data
OTHER model
Sample: Chitin-binding protein CbpD monomer, 39 kDa Pseudomonas aeruginosa protein
Buffer: 15 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Oct 1
The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection. Nat Commun 12(1):1230 (2021)
Askarian F, Uchiyama S, Masson H, Sørensen HV, Golten O, Bunæs AC, Mekasha S, Røhr ÅK, Kommedal E, Ludviksen JA, Arntzen MØ, Schmidt B, Zurich RH, van Sorge NM, Eijsink VGH, Krengel U, Mollnes TE, Lew...
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 64131 nm3

SASDEV5 – Insulin detemir tri-hexamer

Insulin detemir experimental SAS data
SASREF CV model
Sample: Insulin detemir 18-mer, 106 kDa protein
Buffer: 5.0 mM Na2HPO4, 13.1 mM m-cresol, 15.1 mM phenol, 173.7 mM glycerol, 20.0 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 3.3 nm
Dmax 11.3 nm
VolumePorod 137 nm3

SASDJQ5 – Chitin-binding protein CbpD (Pseudomonas aeruginosa) at 24 °C

Chitin-binding protein CbpD experimental SAS data
OTHER model
Sample: Chitin-binding protein CbpD monomer, 39 kDa Pseudomonas aeruginosa protein
Buffer: 15 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar with InCoatec Cu microsource, RECX, University of Oslo on 2019 Jul 19
The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection. Nat Commun 12(1):1230 (2021)
Askarian F, Uchiyama S, Masson H, Sørensen HV, Golten O, Bunæs AC, Mekasha S, Røhr ÅK, Kommedal E, Ludviksen JA, Arntzen MØ, Schmidt B, Zurich RH, van Sorge NM, Eijsink VGH, Krengel U, Mollnes TE, Lew...
RgGuinier 3.3 nm
Dmax 12.1 nm
VolumePorod 50 nm3

SASDEW5 – Albumin-insulin detemir 1:6 complex, binding in Südlow's Site II

Insulin detemir (Levemir(R), Novo Nordisk A/S)Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) experimental SAS data
SASREF CV model
Sample: Insulin detemir (Levemir(R), Novo Nordisk A/S) hexamer, 35 kDa protein
Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Buffer: 6.9 mM Na2HPO4, 11.9 mM m-cresol, 13.7 mM phenol, 157.3 mM glycerol, 38.5 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 162 nm3

SASDJR5 – Chitin-binding protein CbpD (Pseudomonas aeruginosa) at 37°C

Chitin-binding protein CbpD experimental SAS data
Chitin-binding protein CbpD Kratky plot
Sample: Chitin-binding protein CbpD monomer, 39 kDa Pseudomonas aeruginosa protein
Buffer: 15 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar with InCoatec Cu microsource, RECX, University of Oslo on 2019 Aug 14
The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection. Nat Commun 12(1):1230 (2021)
Askarian F, Uchiyama S, Masson H, Sørensen HV, Golten O, Bunæs AC, Mekasha S, Røhr ÅK, Kommedal E, Ludviksen JA, Arntzen MØ, Schmidt B, Zurich RH, van Sorge NM, Eijsink VGH, Krengel U, Mollnes TE, Lew...
RgGuinier 3.5 nm
Dmax 15.0 nm
VolumePorod 63 nm3

SASDEX5 – Albumin-insulin detemir 1:6 complex, binding in Südlow's Site I

Insulin detemir (Levemir(R), Novo Nordisk A/S)Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) experimental SAS data
SASREF CV model
Sample: Insulin detemir (Levemir(R), Novo Nordisk A/S) hexamer, 35 kDa protein
Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Buffer: 6.9 mM Na2HPO4, 11.9 mM m-cresol, 13.7 mM phenol, 157.3 mM glycerol, 38.5 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 162 nm3

SASDEZ5 – Albumin-insulin detemir 2:12 complex, P2 symmetry

Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.)Insulin detemir (Levemir(R), Novo Nordisk A/S) experimental SAS data
SASREF CV model
Sample: Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Insulin detemir (Levemir(R), Novo Nordisk A/S) dodecamer, 71 kDa protein
Buffer: 8.8 mM Na2HPO4, 10.6 mM m-cresol, 12.2 mM phenol, 140.9 mM glycerol, 56.9 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 5.4 nm
Dmax 19.5 nm
VolumePorod 309 nm3

SASDEY5 – Albumin-insulin detemir 2:12 complex, P1 symmetry

Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.)Insulin detemir (Levemir(R), Novo Nordisk A/S) experimental SAS data
SASREF CV model
Sample: Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Insulin detemir (Levemir(R), Novo Nordisk A/S) dodecamer, 71 kDa protein
Buffer: 8.8 mM Na2HPO4, 10.6 mM m-cresol, 12.2 mM phenol, 140.9 mM glycerol, 56.9 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 5.4 nm
Dmax 19.5 nm
VolumePorod 309 nm3