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12 hits found for IgA protease

SASDX42 – IgA peptidase (A31-I1166; active site mutant E540A)

IgA protease (E540A) experimental SAS data
IgA peptidase (A31-I1166; active site mutant E540A) Rg histogram
Sample: IgA protease (E540A) monomer, 128 kDa Thomasclavelia ramosa protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 23
...IgAse provides insight into its unique specificity for the Fab' region of IgA1 and IgA2. PLoS Pathog 21(7):e1013292 (2025)
Ramírez-Larrota JS, Juyoux P, Guerra P, Eckhard U, Gomis-Rüth FX
RgGuinier 5.7 nm
Dmax 27.9 nm
VolumePorod 190 nm3

SASDWH3 – ...protease N-terminal domain

IgA protease experimental SAS data
ALPHAFOLD model
Sample: IgA protease monomer, 32 kDa Thomasclavelia ramosa protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26
...protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases. Proc Natl Acad Sci U S A 122(35):e2503549122 (2025)
Tran N, Frenette A, Holyoak T
RgGuinier 1.9 nm
Dmax 6.0 nm
VolumePorod 38 nm3

SASDWJ3 – ...protease middle (protease) domain

IgA protease experimental SAS data
ALPHAFOLD model
Sample: IgA protease monomer, 55 kDa Thomasclavelia ramosa protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26
...protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases. Proc Natl Acad Sci U S A 122(35):e2503549122 (2025)
Tran N, Frenette A, Holyoak T
RgGuinier 2.6 nm
Dmax 8.8 nm
VolumePorod 82 nm3

SASDWK3 – ...protease middle domain with C-terminal domain #1

IgA protease experimental SAS data
PHENIX model
Sample: IgA protease monomer, 63 kDa Thomasclavelia ramosa protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26
...protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases. Proc Natl Acad Sci U S A 122(35):e2503549122 (2025)
Tran N, Frenette A, Holyoak T
RgGuinier 2.7 nm
Dmax 9.2 nm
VolumePorod 89 nm3

SASDWL3 – ...protease C-terminal domain #2

IgA protease experimental SAS data
ALPHAFOLD model
Sample: IgA protease monomer, 14 kDa Thomasclavelia ramosa protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26
...protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases. Proc Natl Acad Sci U S A 122(35):e2503549122 (2025)
Tran N, Frenette A, Holyoak T
RgGuinier 2.5 nm
Dmax 9.6 nm
VolumePorod 12 nm3

SASDWM3 – ...protease N-terminal domain with middle (protease) domain

IgA protease experimental SAS data
CORAL model
Sample: IgA protease monomer, 88 kDa Thomasclavelia ramosa protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26
...protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases. Proc Natl Acad Sci U S A 122(35):e2503549122 (2025)
Tran N, Frenette A, Holyoak T
RgGuinier 3.3 nm
Dmax 10.8 nm
VolumePorod 108 nm3

SASDWN3 – ...protease C-terminal tail

IgA protease experimental SAS data
CORAL model
Sample: IgA protease monomer, 39 kDa Thomasclavelia ramosa protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26
...protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases. Proc Natl Acad Sci U S A 122(35):e2503549122 (2025)
Tran N, Frenette A, Holyoak T
RgGuinier 5.3 nm
Dmax 21.5 nm
VolumePorod 52 nm3

SASDWP3 – ...protease middle (protease) domain with C-terminal tail

IgA protease experimental SAS data
CORAL model
Sample: IgA protease monomer, 95 kDa Thomasclavelia ramosa protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26
...protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases. Proc Natl Acad Sci U S A 122(35):e2503549122 (2025)
Tran N, Frenette A, Holyoak T
RgGuinier 5.7 nm
Dmax 24.0 nm
VolumePorod 160 nm3

SASDWQ3 – ...protease

IgA protease experimental SAS data
CORAL model
Sample: IgA protease monomer, 131 kDa Thomasclavelia ramosa protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Jun 26
...protease reveals a modular and minimizable architecture distinct from other immunoglobulin A proteases. Proc Natl Acad Sci U S A 122(35):e2503549122 (2025)
Tran N, Frenette A, Holyoak T
RgGuinier 5.5 nm
Dmax 24.5 nm
VolumePorod 188 nm3

SASDX45 – IgA protease (ThomasA) 31-1167

IgA protease experimental SAS data
IgA protease Kratky plot
Sample: IgA protease monomer, 127 kDa Thomasclavelia ramosa protein
Buffer: 50 mM Tris-HCl pH 7.5, 100 mM NaCl, 2% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Apr 19
...IgA antibody recognition by gut-bacterial metallopeptidases The EMBO Journal (2025)
Márquez-Moñino M, Martínez Gascueña A, Azzam T, Persson A, Manzanares-Gomez A, Aguillo-Urarte M, Brown T, Montero-Sagarminaga A, Lood R, Naegeli A, Connell S, Sastre D, Sundberg E, Trastoy B
RgGuinier 6.4 nm
Dmax 25.0 nm
VolumePorod 208 nm3

SASDX55 – IgA protease (ThomasA) 31-878

IgA protease experimental SAS data
IgA protease Kratky plot
Sample: IgA protease monomer, 96 kDa Thomasclavelia ramosa protein
Buffer: 50 mM Tris-HCl pH 7.5, 100 mM NaCl, 2% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Apr 19
...IgA antibody recognition by gut-bacterial metallopeptidases The EMBO Journal (2025)
Márquez-Moñino M, Martínez Gascueña A, Azzam T, Persson A, Manzanares-Gomez A, Aguillo-Urarte M, Brown T, Montero-Sagarminaga A, Lood R, Naegeli A, Connell S, Sastre D, Sundberg E, Trastoy B
RgGuinier 3.4 nm
Dmax 10.6 nm
VolumePorod 101 nm3

SASDX65 – IgA protease (ThomasA) 323-878

IgA protease experimental SAS data
IgA protease Kratky plot
Sample: IgA protease monomer, 64 kDa Thomasclavelia ramosa protein
Buffer: 50 mM Tris-HCl pH 7.5, 100 mM NaCl, 2% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Apr 19
...IgA antibody recognition by gut-bacterial metallopeptidases The EMBO Journal (2025)
Márquez-Moñino M, Martínez Gascueña A, Azzam T, Persson A, Manzanares-Gomez A, Aguillo-Urarte M, Brown T, Montero-Sagarminaga A, Lood R, Naegeli A, Connell S, Sastre D, Sundberg E, Trastoy B
RgGuinier 3.0 nm
Dmax 9.2 nm
VolumePorod 72 nm3