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59 hits found for Apolipoprotein E4

SASDDD7 – Apolipoprotein E2

Apolipoprotein E2 experimental SAS data
Apolipoprotein E2 Kratky plot
Sample: Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Nov 29
...Apolipoprotein E4 as the major risk factor for late onset Alzheimer's disease. J Mol Biol (2019)
Raulin AC, Kraft L, Al-Hilaly YK, Xue WF, McGeehan JE, Atack JR, Serpell L
RgGuinier 5.6 nm
Dmax 19.5 nm
VolumePorod 400 nm3

SASDGJ7 – Apolipoprotein E4 bound to 330 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 7.6 nm
Dmax 26.5 nm

SASDGK7 – Apolipoprotein E4 bound to 110 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 7.6 nm
Dmax 27.0 nm

SASDGL7 – Apolipoprotein E4 bound to 37 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 7.0 nm
Dmax 25.8 nm

SASDGM7 – Apolipoprotein E4 bound to 12 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.7 nm
Dmax 22.0 nm

SASDGN7 – Apolipoprotein E4 bound to 4 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.4 nm
Dmax 21.2 nm

SASDGP7 – ...Apolipoprotein E4 (K143A K146A) bound to 330 µg/mL heparin

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.2 nm
Dmax 20.8 nm

SASDGQ7 – ...Apolipoprotein E4 (K143A K146A) bound to 110 µg/mL heparin

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.1 nm
Dmax 21.2 nm

SASDGR7 – ...Apolipoprotein E4 (K143A K146A) bound to 37 µg/mL heparin

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.2 nm
Dmax 21.3 nm

SASDGS7 – ...Apolipoprotein E4 (K143A K146A) bound to 12 µg/mL heparin

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.1 nm
Dmax 20.5 nm

SASDGT7 – ...Apolipoprotein E4 (K143A K146A) bound to 4 µg/mL heparin

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.1 nm
Dmax 20.5 nm

SASDGU7 – ...Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 3.1 nm
Dmax 10.5 nm

SASDGV7 – ...Apolipoprotein E4 (1-191) bound to 37 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 2.6 nm
Dmax 8.2 nm

SASDGW7 – ...Apolipoprotein E4 (1-191) bound to 12 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 2.5 nm
Dmax 7.8 nm

SASDGX7 – ...Apolipoprotein E4 (1-191) bound to 4 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 2.4 nm
Dmax 8.0 nm

SASDGY7 – Apolipoprotein E4 (methylated) bound to 330 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.9 nm
Dmax 23.7 nm

SASDGZ7 – Apolipoprotein E4 (methylated) bound to 110 µg/mL heparin

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.8 nm
Dmax 23.7 nm

SASDG28 – Apolipoprotein E4 (methylated) bound to 37 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.4 nm
Dmax 22.2 nm

SASDG38 – Apolipoprotein E4 (methylated) bound to 12 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.3 nm
Dmax 20.8 nm

SASDG48 – Apolipoprotein E4 (methylated) bound to 4 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.1 nm
Dmax 20.2 nm

SASDD98 – Apolipoprotein E3

Apolipoprotein E3 experimental SAS data
Apolipoprotein E3 Kratky plot
Sample: Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Nov 29
...Apolipoprotein E4 as the major risk factor for late onset Alzheimer's disease. J Mol Biol (2019)
Raulin AC, Kraft L, Al-Hilaly YK, Xue WF, McGeehan JE, Atack JR, Serpell L
RgGuinier 5.7 nm
Dmax 19.5 nm
VolumePorod 410 nm3

SASDDA8 – Apolipoprotein E4

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Nov 29
...Apolipoprotein E4 as the major risk factor for late onset Alzheimer's disease. J Mol Biol (2019)
Raulin AC, Kraft L, Al-Hilaly YK, Xue WF, McGeehan JE, Atack JR, Serpell L
RgGuinier 5.8 nm
Dmax 19.6 nm
VolumePorod 430 nm3

SASDGF8 – Apolipoprotein E4 bound to 330 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.4 nm
Dmax 19.6 nm

SASDGG8 – Apolipoprotein E4 bound to 110 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.5 nm
Dmax 19.5 nm

SASDGH8 – Apolipoprotein E4 bound to 37 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.0 nm
Dmax 20.1 nm

SASDGJ8 – Apolipoprotein E4 bound to 12 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.1 nm
Dmax 20.1 nm

SASDGK8 – Apolipoprotein E4 bound to 4 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.2 nm
Dmax 20.4 nm

SASDGL8 – Apolipoprotein E4 (K143A K146A) mutant bound to 330 µM Suramin

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.0 nm
Dmax 22.4 nm

SASDGM8 – Apolipoprotein E4 (K143A K146A) mutant bound to 110 µM Suramin

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.5 nm
Dmax 18.9 nm

SASDGN8 – Apolipoprotein E4 (K143A K146A) mutant bound to 37 µM Suramin

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.9 nm
Dmax 19.9 nm

SASDGP8 – Apolipoprotein E4 (K143A K146A) mutant bound to12 µM Suramin

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.0 nm
Dmax 20.2 nm

SASDGQ8 – Apolipoprotein E4 (K143A K146A) mutant bound to 4 µM Suramin

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.1 nm
Dmax 20.4 nm

SASDGR8 – Apolipoprotein E4 (methylated) bound to 330 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.0 nm
Dmax 20.6 nm

SASDGS8 – Apolipoprotein E4 (methylated) bound to 110 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.7 nm
Dmax 18.1 nm

SASDGT8 – Apolipoprotein E4 (methylated) bound to 37 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.8 nm
Dmax 19.6 nm

SASDGU8 – Apolipoprotein E4 (methylated) bound to 12 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.0 nm
Dmax 19.4 nm

SASDGV8 – Apolipoprotein E4 (methylated) bound to 4 µM Suramin

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.0 nm
Dmax 19.7 nm

SASDGW8 – ...Apolipoprotein E4 (1-191) bound to 330 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 3.1 nm
Dmax 11.1 nm

SASDGX8 – ...Apolipoprotein E4 (1-191) bound to 110 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 2.8 nm
Dmax 9.5 nm

SASDGY8 – ...Apolipoprotein E4 (1-191) bound to 37 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 2.6 nm
Dmax 8.0 nm

SASDGZ8 – ...Apolipoprotein E4 (1-191) bound to 12 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 2.5 nm
Dmax 8.2 nm

SASDG29 – ...Apolipoprotein E4 (1-191) bound to 4 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 2.4 nm
Dmax 7.8 nm

SASDG59 – Apolipoprotein E4

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.2 nm
Dmax 20.8 nm

SASDG69 – Apolipoprotein E4 (K143A K146A) mutant

Apolipoprotein E4 (K143A K146A) mutant experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.2 nm
Dmax 20.7 nm

SASDG79 – Apolipoprotein E4 (methylated)

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.1 nm
Dmax 20.3 nm

SASDG89 – ...Apolipoprotein E4 (1-191)

Apolipoprotein E4 (1-191) experimental SAS data
Apolipoprotein E4 (1-191) Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 2.4 nm
Dmax 7.7 nm

SASDGF9 – Apolipoprotein E4 (SEC-SAXS)

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.9 nm
Dmax 20.0 nm

SASDGG9 – Apolipoprotein E4 bound to 1 mg/mL heparin (SEC-SAXS)

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.2 nm
Dmax 21.6 nm

SASDGH9 – Apolipoprotein E4 bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.6 nm
Dmax 18.7 nm

SASDGJ9 – Apolipoprotein E4 (methylated) - (SEC-SAXS)

Apolipoprotein E4 experimental SAS data
Apolipoprotein E4 Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.9 nm
Dmax 20.0 nm

SASDGK9 – Apolipoprotein E4 (methylated) bound to 1 mg/mL heparin (SEC-SAXS)

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 6.4 nm
Dmax 21.7 nm

SASDGL9 – Apolipoprotein E4 (methylated) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.3 nm
Dmax 18.5 nm

SASDGM9 – ...Apolipoprotein E4 (K143A K146A) - (SEC-SAXS)

Apolipoprotein E4 (K143A K146A) mutant experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.8 nm
Dmax 20.2 nm

SASDGN9 – ...Apolipoprotein E4 (K143A K146A) bound to 1 mg/mL heparin (SEC-SAXS)

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.7 nm
Dmax 19.8 nm

SASDGP9 – ...Apolipoprotein E4 (K143A K146A) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Apolipoprotein E project
Lucas Kraft
RgGuinier 5.4 nm
Dmax 18.3 nm

SASDGQ9 – ...Apolipoprotein E4 (1-191) - (SEC-SAXS)

Apolipoprotein E4 (1-191) experimental SAS data
Apolipoprotein E4 (1-191) Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 2.3 nm
Dmax 7.6 nm

SASDGR9 – ...Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC-SAXS)

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 4.2 nm
Dmax 16.8 nm

SASDGS9 – ...Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 2.4 nm
Dmax 8.3 nm

SASDGT9 – ...Apolipoprotein E4 (1-191) bound to 110 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Apolipoprotein E project
Lucas Kraft
RgGuinier 2.7 nm
Dmax 8.3 nm