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20 hits found for Barbosa

SASDL42 – Human Heat Shock Cognate 71 kDa Protein (HSPA8 / Hsc70)

Heat shock cognate 71 kDa protein experimental SAS data
Human Heat Shock Cognate 71 kDa Protein (HSPA8 / Hsc70) Rg histogram
Sample: Heat shock cognate 71 kDa protein monomer, 71 kDa Homo sapiens protein
Buffer: 25 mM Tris HCl, 50 mM NaCl, 5 mM Sodium Phosphate, 5 mM KCl, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 18
Structural, thermodynamic and functional studies of human 71 kDa heat shock cognate protein (HSPA8/hHsc70) Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics :140719 (2021)
...Barbosa L, Borges J
RgGuinier 3.7 nm
Dmax 11.5 nm
VolumePorod 111 nm3

SASDL52 – Human Heat Shock 70 kDa Protein 1A (Hsp70-1A/HSPA1A)

Heat shock 70 kDa protein 1A experimental SAS data
DAMMIN model
Sample: Heat shock 70 kDa protein 1A monomer, 70 kDa Homo sapiens protein
Buffer: 25 mM Tris HCl, 50 mM NaCl, 5 mM Sodium Phosphate, 5 mM KCl, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 18
Structural, thermodynamic and functional studies of human 71 kDa heat shock cognate protein (HSPA8/hHsc70) Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics :140719 (2021)
...Barbosa L, Borges J
RgGuinier 3.7 nm
Dmax 11.0 nm
VolumePorod 138 nm3

SASDBD3 – Leishmania braziliensis heat shock protein 90 (Hsp90).

Leishmania braziliensis heat shock protein 90 (Hsp90) experimental SAS data
DAMMIN model
Sample: Leishmania braziliensis heat shock protein 90 (Hsp90) dimer, 166 kDa Leishmania braziliensis protein
Buffer: 25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2011 Sep 1
Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering. Int J Biol Macromol 97:503-512 (2017)
...Barbosa LR, Borges JC
RgGuinier 5.3 nm
Dmax 21.0 nm
VolumePorod 380 nm3

SASDBE3 – Leishmania braziliensis heat shock protein 90 (Hsp90) N domain.

Leishmania braziliensis heat shock protein 90 (Hsp90) N domain experimental SAS data
DAMMIN model
Sample: Leishmania braziliensis heat shock protein 90 (Hsp90) N domain monomer, 26 kDa Leishmania braziliensis protein
Buffer: 25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2011 Sep 1
Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering. Int J Biol Macromol 97:503-512 (2017)
...Barbosa LR, Borges JC
RgGuinier 2.0 nm
Dmax 7.5 nm
VolumePorod 40 nm3

SASDBF3 – Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains.

Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains experimental SAS data
DAMMIN model
Sample: Leishmania braziliensis heat shock protein 90 (Hsp90) N and M domains monomer, 62 kDa Leishmania braziliensis protein
Buffer: 25 Tris mM 100 mM NaCl 1 mM 2-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2011 Sep 1
Insights on the structural dynamics of Leishmania braziliensis Hsp90 molecular chaperone by small angle X-ray scattering. Int J Biol Macromol 97:503-512 (2017)
...Barbosa LR, Borges JC
RgGuinier 3.2 nm
Dmax 13.0 nm
VolumePorod 89 nm3

SASDB54 – Leishmania braziliensis stress-induced protein sti1 (LbHop), full length construct

Stress-induced protein sti1 experimental SAS data
DAMFILT model
Sample: Stress-induced protein sti1 monomer, 62 kDa Leishmania braziliensis protein
Buffer: 25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Feb 21
Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis. Arch Biochem Biophys 600:12-22 (2016)
...Barbosa LRS, Ramos CHI, Borges JC
RgGuinier 4.5 nm
Dmax 18.0 nm
VolumePorod 94 nm3

SASDB64 – Leishmania braziliensis stress-induced protein sti1 (LbHop TPR2A-TPR2B-DP2 construct)

Stress-induced protein sti1 (Hop TPR2A-TPR2B-DP2 construct) experimental SAS data
DAMFILT model
Sample: Stress-induced protein sti1 (Hop TPR2A-TPR2B-DP2 construct) monomer, 44 kDa Leishmania braziliensis protein
Buffer: 25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Feb 21
Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis. Arch Biochem Biophys 600:12-22 (2016)
...Barbosa LRS, Ramos CHI, Borges JC
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 65 nm3

SASDBR4 – Leishmania braziliensis Activator of Hsp90 ATPase-1 (LbAha1)

Activator of Hsp90 ATPase-1 experimental SAS data
Leishmania braziliensis Activator of Hsp90 ATPase-1 (LbAha1) Rg histogram
Sample: Activator of Hsp90 ATPase-1 monomer, 38 kDa Leishmania braziliensis protein
Buffer: 25 mM Sodium phosphate, 50 mM NaCl, 2 mM EDTA, 1 mM β-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2013 Jun 1
Low resolution structural studies indicate that the activator of Hsp90 ATPase 1 (Aha1) of Leishmania braziliensis has an elongated shape which allows its interaction with both N- and M-domains of Hsp9... PLoS One 8(6):e66822 (2013)
...Barbosa LR, Borges JC
RgGuinier 3.6 nm
Dmax 14.5 nm

SASDC55 – Plasmodium falciparum p23A

CS domain protein, putative experimental SAS data
DAMFILT model
Sample: CS domain protein, putative monomer, 19 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 2 mM EDTA, 1 mM B-mercaptoethanol, pH: 7.4
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 May 26
Comparative studies of the low-resolution structure of two p23 co-chaperones for Hsp90 identified in Plasmodium falciparum genome. Int J Biol Macromol 108:193-204 (2018)
...Barbosa LRS, Borges JC
RgGuinier 2.5 nm
Dmax 8.5 nm

SASDC65 – Plasmodium falciparum p23B

Co-chaperone p23 experimental SAS data
DAMFILT model
Sample: Co-chaperone p23 monomer, 31 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 2 mM EDTA, 1 mM B-mercaptoethanol, pH: 7.4
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 May 26
Comparative studies of the low-resolution structure of two p23 co-chaperones for Hsp90 identified in Plasmodium falciparum genome. Int J Biol Macromol 108:193-204 (2018)
...Barbosa LRS, Borges JC
RgGuinier 3.7 nm
Dmax 13.0 nm

SASDC85 – Leishmania braziliensis p23B

Leishmania braziliensis p23 isoform B experimental SAS data
DAMFILT model
Sample: Leishmania braziliensis p23 isoform B monomer, 23 kDa Leishmania braziliensis protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 8
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Mar 26
Identification of two p23 co-chaperone isoforms in Leishmania braziliensis exhibiting similar structures and Hsp90 interaction properties despite divergent stabilities. FEBS J 282(2):388-406 (2015)
...Barbosa LR, Borges JC
RgGuinier 3.0 nm
Dmax 13.0 nm

SASDFL5 – Plasmodium falciparum Hsp70/Hsp90 organizing protein, Hop

STI1-like protein experimental SAS data
DAMFILT model
Sample: STI1-like protein dimer, 136 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 1 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Aug 3
Structural studies of the Hsp70/Hsp90 organizing protein of Plasmodium falciparum and its modulation of Hsp70 and Hsp90 ATPase activities. Biochim Biophys Acta Proteins Proteom :140282 (2019)
...Barbosa LRS, Borges JC
RgGuinier 6.3 nm
Dmax 24.0 nm
VolumePorod 557 nm3

SASDCV5 – Leishmania braziliensis p23A

Uncharacterized protein experimental SAS data
DAMFILT model
Sample: Uncharacterized protein monomer, 22 kDa Leishmania braziliensis protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 8
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Mar 26
Identification of two p23 co-chaperone isoforms in Leishmania braziliensis exhibiting similar structures and Hsp90 interaction properties despite divergent stabilities. FEBS J 282(2):388-406 (2015)
...Barbosa LR, Borges JC
RgGuinier 3.3 nm
Dmax 13.0 nm

SASDHD9 – Full-length Plasmodium falciparum Heat shock protein 90 (PfHsp90)

Plasmodium falciparum Heat shock protein 90 experimental SAS data
DAMMIN model
Sample: Plasmodium falciparum Heat shock protein 90 dimer, 177 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM KCl, 1 mM β-mercaptoethanol, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 11
Solution structure of Plasmodium falciparum Hsp90 indicates a high flexible dimer Archives of Biochemistry and Biophysics :108468 (2020)
...Barbosa L, Seraphim T, Borges J
RgGuinier 5.7 nm
Dmax 19.0 nm
VolumePorod 350 nm3

SASDHE9 – Plasmodium falciparum Heat shock protein 90 (PfHsp90) N- and M-domains

Plasmodium falciparum Heat shock protein 90 N-terminal and Middle domains experimental SAS data
Plasmodium falciparum Heat shock protein 90 (PfHsp90) N- and M-domains Rg histogram
Sample: Plasmodium falciparum Heat shock protein 90 N-terminal and Middle domains monomer, 68 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM KCl, 1 mM β-mercaptoethanol, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 11
Solution structure of Plasmodium falciparum Hsp90 indicates a high flexible dimer Archives of Biochemistry and Biophysics :108468 (2020)
...Barbosa L, Seraphim T, Borges J
RgGuinier 3.9 nm
Dmax 14.0 nm
VolumePorod 106 nm3

SASDHF9 – Plasmodium falciparum Heat shock protein 90 (PfHsp90) M-domain

Plasmodium falciparum Heat shock protein 90 middle domain experimental SAS data
DAMMIN model
Sample: Plasmodium falciparum Heat shock protein 90 middle domain monomer, 33 kDa Plasmodium falciparum protein
Buffer: 25 mM Tris-HCl, 100 mM KCl, 1 mM β-mercaptoethanol, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 11
Solution structure of Plasmodium falciparum Hsp90 indicates a high flexible dimer Archives of Biochemistry and Biophysics :108468 (2020)
...Barbosa L, Seraphim T, Borges J
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 47 nm3

SASDBH6 – Full-length human p23 (1-160)

Prostaglandin E synthase 3 experimental SAS data
Prostaglandin E synthase 3 Kratky plot
Sample: Prostaglandin E synthase 3 monomer, 19 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Jun 22
The C-terminal region of the human p23 chaperone modulates its structure and function. Arch Biochem Biophys 565:57-67 (2015)
...Barbosa LR, Ramos CH, Borges JC
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 40 nm3

SASDBJ6 – Truncated construct of human p23 (1-142)

Prostaglandin E synthase 3 (1-142) experimental SAS data
Prostaglandin E synthase 3 (1-142) Kratky plot
Sample: Prostaglandin E synthase 3 (1-142) monomer, 17 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Jun 22
The C-terminal region of the human p23 chaperone modulates its structure and function. Arch Biochem Biophys 565:57-67 (2015)
...Barbosa LR, Ramos CH, Borges JC
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 36 nm3

SASDBL6 – Truncated construct of human p23 (1-117)

Prostaglandin E synthase 3 (1-117) experimental SAS data
Prostaglandin E synthase 3 (1-117) Kratky plot
Sample: Prostaglandin E synthase 3 (1-117) monomer, 14 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2013 Jun 21
The C-terminal region of the human p23 chaperone modulates its structure and function. Arch Biochem Biophys 565:57-67 (2015)
...Barbosa LR, Ramos CH, Borges JC
RgGuinier 1.9 nm
Dmax 7.0 nm
VolumePorod 29 nm3

SASDBK6 – Truncated construct of human p23 (1-131)

Prostaglandin E synthase 3 (1-131) experimental SAS data
Prostaglandin E synthase 3 (1-131) Kratky plot
Sample: Prostaglandin E synthase 3 (1-131) monomer, 16 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2013 Jun 21
The C-terminal region of the human p23 chaperone modulates its structure and function. Arch Biochem Biophys 565:57-67 (2015)
...Barbosa LR, Ramos CH, Borges JC
RgGuinier 1.9 nm
Dmax 7.0 nm
VolumePorod 31 nm3