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8 hits found for Butt

SASDE52 – Ribonuclease E from Escherichia coli

Endoribonuclease E experimental SAS data
DAMFILT model
Sample: Endoribonuclease E tetramer, 247 kDa Escherichia coli protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
...Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.0 nm
Dmax 16.1 nm
VolumePorod 468 nm3

SASDE62 – Ribonuclease E from Yersinia pestis

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 248 kDa Yersinia pestis protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
...Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.1 nm
Dmax 16.4 nm
VolumePorod 470 nm3

SASDE72 – Ribonuclease E from Francisella tularensis

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 256 kDa Francisella tularensis protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
...Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.1 nm
Dmax 17.2 nm
VolumePorod 491 nm3

SASDE82 – Ribonuclease E from Burkholderia pseudomallei

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 250 kDa Burkholderia pseudomallei protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
...Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 4.8 nm
Dmax 14.9 nm
VolumePorod 437 nm3

SASDE92 – Ribonuclease E from Acinetobacter baumannii

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 254 kDa Acinetobacter baumannii protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
...Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.2 nm
Dmax 18.3 nm
VolumePorod 508 nm3

SASDG37 – 1:2 heterotrimer of pUL7 and pUL51(8-142) from herpes simplex virus 1

Tegument protein UL7Tegument protein UL51 experimental SAS data
DAMMIN model
Sample: Tegument protein UL7 monomer, 34 kDa Human alphaherpesvirus 1 … protein
Tegument protein UL51 dimer, 30 kDa Human alphaherpesvirus 1 … protein
Buffer: 20 mM tris, 200 mM NaCl, 3% (v/v) glycerol, 0.25 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 16
Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex. Elife 9 (2020)
Butt BG, Owen DJ, Jeffries CM, Ivanova L, Hill CH, Houghton JW, Ahmed MF, Antrobus R, Svergun DI, Welch JJ, Crump CM, Graham SC
RgGuinier 3.0 nm
Dmax 11.5 nm
VolumePorod 116 nm3

SASDG47 – 2:4 heterohexamer of pUL7 and pUL51 from herpes simplex virus 1

Tegument protein UL7Tegument protein UL51 experimental SAS data
DAMMIN model
Sample: Tegument protein UL7 dimer, 68 kDa Human alphaherpesvirus 1 … protein
Tegument protein UL51 tetramer, 102 kDa Human alphaherpesvirus 1 … protein
Buffer: 20 mM HEPES, 200 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 16
Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex. Elife 9 (2020)
Butt BG, Owen DJ, Jeffries CM, Ivanova L, Hill CH, Houghton JW, Ahmed MF, Antrobus R, Svergun DI, Welch JJ, Crump CM, Graham SC
RgGuinier 4.6 nm
Dmax 19.7 nm
VolumePorod 340 nm3

SASDG57 – 1:2 heterotrimer of pUL7 and pUL51 from herpes simplex virus 1

Tegument protein UL7Tegument protein UL51 experimental SAS data
GASBOR model
Sample: Tegument protein UL7 monomer, 34 kDa Human alphaherpesvirus 1 … protein
Tegument protein UL51 dimer, 51 kDa Human alphaherpesvirus 1 protein
Buffer: 20 mM HEPES, 200 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 16
Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex. Elife 9 (2020)
Butt BG, Owen DJ, Jeffries CM, Ivanova L, Hill CH, Houghton JW, Ahmed MF, Antrobus R, Svergun DI, Welch JJ, Crump CM, Graham SC
RgGuinier 4.0 nm
Dmax 18.2 nm
VolumePorod 160 nm3