SASBDB entries for UniProt ID:

SASDHK9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and UDP-glucose

UniProt ID: F2L613 (1-401) Trehalose transferase (Trehalose phosphorylase/synthase)
Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM UDP-glucose, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 73 nm3

SASDHQ9 – Proliferating cell nuclear antigen (PCNA) from pathogenic yeast Candida albicans

UniProt ID: Q5AMN0 (1-259) Proliferating cell nuclear antigen (C-terminal His-tagged)
Proliferating cell nuclear antigen (C-terminal His-tagged) experimental SAS data
GASBOR model
Sample: Proliferating cell nuclear antigen (C-terminal His-tagged) trimer, 90 kDa Candida albicans protein
Buffer: 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Sep 3
Structural analyses of PCNA from the fungal pathogen Candida albicans identify three regions with species-specific conformations. FEBS Lett (2021)
Sundaram R, Manohar K, Patel SK, Acharya N, Vasudevan D
RgGuinier 3.4 nm
Dmax 10.2 nm
VolumePorod 128 nm3

SASDHR9 – GntR-type transcriptional regulator NanR from Escherichia coli

UniProt ID: P0A8W0 (1-263) HTH-type transcriptional repressor NanR
HTH-type transcriptional repressor NanR experimental SAS data
CUSTOM IN-HOUSE model
Sample: HTH-type transcriptional repressor NanR dimer, 59 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.1 % (w/v) sodium azide, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Apr 20
Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism (2020)
Horne C, Venugopal H, Panjikar S, Henrickson A, Brookes E, North R, Murphy J, Friemann R, Griffin M, Ramm G, Demeler B, Dobson R
RgGuinier 3.2 nm
Dmax 10.5 nm
VolumePorod 110 nm3

SASDHS9 – GntR-type transcriptional regulator NanR from Escherichia coli in the presence of N-acetylneuraminic acid

UniProt ID: P0A8W0 (1-263) HTH-type transcriptional repressor NanR
HTH-type transcriptional repressor NanR experimental SAS data
CUSTOM IN-HOUSE model
Sample: HTH-type transcriptional repressor NanR dimer, 59 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 20 mM Neu5Ac and 0.1 % (w/v) sodium azide, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Apr 20
Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism (2020)
Horne C, Venugopal H, Panjikar S, Henrickson A, Brookes E, North R, Murphy J, Friemann R, Griffin M, Ramm G, Demeler B, Dobson R
RgGuinier 3.1 nm
Dmax 10.1 nm
VolumePorod 105 nm3

SASDHT9 – Dimeric NanR-DNA hetero-complex from Escherichia coli

UniProt ID: P0A8W0 (1-263) HTH-type transcriptional repressor NanR

UniProt ID: None (None-None) (GGTATA)2 repeat DNA
HTH-type transcriptional repressor NanR(GGTATA)2 repeat DNA experimental SAS data
CUSTOM IN-HOUSE model
Sample: HTH-type transcriptional repressor NanR dimer, 59 kDa Escherichia coli protein
(GGTATA)2 repeat DNA monomer, 11 kDa DNA
Buffer: 20 mM Tris, 150 mM NaCl, 0.1 % (w/v) sodium azide, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Apr 20
Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism (2020)
Horne C, Venugopal H, Panjikar S, Henrickson A, Brookes E, North R, Murphy J, Friemann R, Griffin M, Ramm G, Demeler B, Dobson R
RgGuinier 3.3 nm
Dmax 9.8 nm
VolumePorod 108 nm3

SASDHU9 – Solution structure of middle domain of the multi-domain GH18 chitinase ChiA from Flavobacterium johnsoniae

UniProt ID: A5FB63 (464-1137) Chitinase ChiA
Chitinase ChiA experimental SAS data
DAMMIN model
Sample: Chitinase ChiA monomer, 70 kDa Flavobacterium johnsoniae protein
Buffer: 50 mM Tris, 250 mM NaCl, 0.25 mM DTT, pH: 8
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2019 May 7
Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae. Sci Rep 10(1):13775 (2020)
Mazurkewich S, Helland R, Mackenzie A, Eijsink VGH, Pope PB, Brändén G, Larsbrink J
RgGuinier 4.7 nm
Dmax 16.3 nm
VolumePorod 99 nm3

SASDHV9 – Murine major prion protein PrPc 23-230

UniProt ID: P04925 (23-230) Major prion protein
Major prion protein experimental SAS data
CORAL model
Sample: Major prion protein monomer, 23 kDa Mus musculus protein
Buffer: 10mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Dec 15
Characterization of murine prion protein
Stefano Da Vela
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 43 nm3

SASDHZ9 – Condensin complex subunit 1, Ycs4

UniProt ID: G0SB82 (3-1222) Condensin complex subunit 1
Condensin complex subunit 1 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos
RgGuinier 5.2 nm
Dmax 17.7 nm
VolumePorod 296 nm3

SASDJ22 – Condensin complex subunit 1 (Ycs4) bound to condensin complex subunit 2, 336-418 (Brn1S)

UniProt ID: G0SB82 (3-1222) Condensin complex subunit 1

UniProt ID: G0SBJ6 (336-418) Condensin complex subunit 2, 336-418
Condensin complex subunit 1Condensin complex subunit 2, 336-418 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 336-418 monomer, 9 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos
RgGuinier 5.0 nm
Dmax 16.9 nm
VolumePorod 279 nm3

SASDJ32 – Condensin complex subunit 1 (Ycs4) bound to condensin complex subunit 2, 225-418 (Brn1L)

UniProt ID: G0SB82 (3-1222) Condensin complex subunit 1

UniProt ID: G0SBJ6 (225-418) Condensin complex subunit 2, 225-418
Condensin complex subunit 1Condensin complex subunit 2, 225-418 experimental SAS data
MONSA model
Sample: Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Condensin complex subunit 2, 225-418 monomer, 21 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20
Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos
RgGuinier 4.9 nm
Dmax 15.9 nm
VolumePorod 309 nm3