SASBDB entries for UniProt ID:

SASDJ98 – Jagged1 EGF8-11 58 µM

UniProt ID: Q9QXX0 (487-665) Protein jagged-1 EGF8-11
Protein jagged-1 EGF8-11 experimental SAS data
Protein jagged-1 EGF8-11 Kratky plot
Sample: Protein jagged-1 EGF8-11, 21 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 31 nm3

SASDJA8 – Jagged1 EGF8-11 115 µM

UniProt ID: Q9QXX0 (487-665) Protein jagged-1 EGF8-11
Protein jagged-1 EGF8-11 experimental SAS data
Protein jagged-1 EGF8-11 Kratky plot
Sample: Protein jagged-1 EGF8-11, 21 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 3.1 nm
Dmax 11.5 nm
VolumePorod 34 nm3

SASDJB8 – Jagged1 EGF8-11 230 µM

UniProt ID: Q9QXX0 (487-665) Protein jagged-1 EGF8-11
Protein jagged-1 EGF8-11 experimental SAS data
Protein jagged-1 EGF8-11 Kratky plot
Sample: Protein jagged-1 EGF8-11, 21 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 66 nm3

SASDJC8 – Jagged1 cysteine-rich domain (CRD) 21 µM

UniProt ID: Q9QXX0 (857-1067) Protein jagged-1 cysteine-rich domain
Protein jagged-1 cysteine-rich domain experimental SAS data
Protein jagged-1 cysteine-rich domain Kratky plot
Sample: Protein jagged-1 cysteine-rich domain, 24 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 2.3 nm
Dmax 7.5 nm
VolumePorod 51 nm3

SASDJD8 – Jagged1 cysteine-rich domain (CRD) 42 µM

UniProt ID: Q9QXX0 (857-1067) Protein jagged-1 cysteine-rich domain
Protein jagged-1 cysteine-rich domain experimental SAS data
Protein jagged-1 cysteine-rich domain Kratky plot
Sample: Protein jagged-1 cysteine-rich domain, 24 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 2.3 nm
Dmax 7.8 nm
VolumePorod 56 nm3

SASDJE8 – Jagged1 cysteine-rich domain (CRD) 83 µM

UniProt ID: Q9QXX0 (857-1067) Protein jagged-1 cysteine-rich domain
Protein jagged-1 cysteine-rich domain experimental SAS data
Protein jagged-1 cysteine-rich domain Kratky plot
Sample: Protein jagged-1 cysteine-rich domain, 24 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 2.3 nm
Dmax 8.2 nm
VolumePorod 59 nm3

SASDJF8 – Jagged1 cysteine-rich domain (CRD) 167 µM

UniProt ID: Q9QXX0 (857-1067) Protein jagged-1 cysteine-rich domain
Protein jagged-1 cysteine-rich domain experimental SAS data
Protein jagged-1 cysteine-rich domain Kratky plot
Sample: Protein jagged-1 cysteine-rich domain, 24 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 2.4 nm
Dmax 9.0 nm
VolumePorod 61 nm3

SASDJG8 – Monomeric neurogenic locus notch homolog protein 1 (Notch1) full ectodomain

UniProt ID: P46531 (19-1728) Neurogenic locus notch homolog protein 1 full ectodomain
Neurogenic locus notch homolog protein 1 full ectodomain experimental SAS data
Neurogenic locus notch homolog protein 1 full ectodomain Kratky plot
Sample: Neurogenic locus notch homolog protein 1 full ectodomain monomer, 183 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 25
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 10.5 nm
Dmax 38.0 nm
VolumePorod 1000 nm3

SASDJH8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, wild-type) concentration series data

UniProt ID: Q9UBF9 (220-452) Myotilin (222-452)
Myotilin (222-452) experimental SAS data
Myotilin (222-452) Kratky plot
Sample: Myotilin (222-452) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jul 6
Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L
RgGuinier 3.3 nm
Dmax 11.5 nm
VolumePorod 36 nm3

SASDJJ8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, R405K mutant) concentration series data

UniProt ID: Q9UBF9 (222-452) Myotilin (222-452) R405K
Myotilin (222-452) R405K experimental SAS data
Myotilin (222-452) R405K Kratky plot
Sample: Myotilin (222-452) R405K monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jul 12
Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 36 nm3