SASBDB entries for UniProt ID:

SASDJT7 – Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5

UniProt ID: Q9C7W1 (1-178) Filaggrin-like protein
Filaggrin-like protein experimental SAS data
Phloem-associated RNA chaperone-like protein (PARCL) at pH 6.5 Rg histogram
Sample: Filaggrin-like protein monomer, 20 kDa Arabidopsis thaliana protein
Buffer: 50 mM HEPES, 150 mM NaCl, 5 mM DTT, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 22
Intrinsically disordered plant protein PARCL co-localizes with RNA in phase-separated condensates whose formation can be regulated by mutating the PLD Journal of Biological Chemistry :102631 (2022)
Ostendorp A, Ostendorp S, Zhou Y, Chaudron Z, Wolffram L, Rombi K, von Pein L, Falke S, Jeffries C, Svergun D, Betzel C, Morris R, Kragler F, Kehr J
RgGuinier 3.7 nm
Dmax 15.0 nm
VolumePorod 48 nm3

SASDJW7 – pri-miR16-1 primary microRNA in complex with DGCR8-core protein

UniProt ID: None (None-None) primary microRNA pri-miR16-1 complexed with DGCR8-core protein

UniProt ID: Q8WYQ5 (493-720) Microprocessor complex subunit DGCR8
primary microRNA pri-miR16-1 complexed with DGCR8-core proteinMicroprocessor complex subunit DGCR8 experimental SAS data
DAMMIF model
Sample: Primary microRNA pri-miR16-1 complexed with DGCR8-core protein monomer, 36 kDa Homo sapiens RNA
Microprocessor complex subunit DGCR8 monomer, 26 kDa Homo sapiens protein
Buffer: 50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, 50% sucrose, DGCR8-core, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures Biophysical Journal (2020)
Pabit S, Chen Y, Usher E, Cook E, Pollack L, Showalter S
RgGuinier 5.0 nm
Dmax 17.2 nm
VolumePorod 125 nm3

SASDJX7 – Labrum-Interacting Proteins from Saliva 2 (34k-2) - SEC-SAXS

UniProt ID: Q5MIU2 (21-322) Similar to Aedes aegypti 34 kDa salivary secreted protein 34k-2
Similar to Aedes aegypti 34 kDa salivary secreted protein 34k-2 experimental SAS data
ALLOSMOD model
Sample: Similar to Aedes aegypti 34 kDa salivary secreted protein 34k-2 monomer, 35 kDa Aedes albopictus protein
Buffer: 25 mM HEPES, 0.1 M NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2016 Sep 3
A salivary factor binds a cuticular protein and modulates biting by inducing morphological changes in the mosquito labrum. Curr Biol (2022)
Arnoldi I, Mancini G, Fumagalli M, Gastaldi D, D'Andrea L, Bandi C, Di Venere M, Iadarola P, Forneris F, Gabrieli P
RgGuinier 2.7 nm
Dmax 8.4 nm
VolumePorod 50 nm3

SASDJ28 – Ferric Iron Reductase - FhuF∆1-17

UniProt ID: P39405 (18-262) Ferric iron reductase protein FhuF (∆1-17)
Ferric iron reductase protein FhuF (∆1-17) experimental SAS data
DAMFILT model
Sample: Ferric iron reductase protein FhuF (∆1-17) monomer, 28 kDa Escherichia coli (strain … protein
Buffer: 20 mM Phosphate, 200 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 16
Conjuring up a ghost: structural and functional characterization of FhuF, a ferric siderophore reductase from E. coli JBIC Journal of Biological Inorganic Chemistry (2021)
Trindade I, Hernandez G, Lebègue E, Barrière F, Cordeiro T, Piccioli M, Louro R
RgGuinier 2.1 nm
Dmax 8.8 nm
VolumePorod 60 nm3

SASDJ38 – Monomeric Jagged1 high-affinity full ectodomain

UniProt ID: Q9QXX0 (31-1067) Protein jagged-1
Protein jagged-1 experimental SAS data
Protein jagged-1 Kratky plot
Sample: Protein jagged-1 monomer, 115 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 12
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 7.4 nm
Dmax 24.0 nm
VolumePorod 375 nm3

SASDJ48 – Jagged1 high-affinity full ectodomain 5.3 µM

UniProt ID: Q9QXX0 (31-1067) Protein jagged-1
Protein jagged-1 experimental SAS data
Protein jagged-1 Kratky plot
Sample: Protein jagged-1, 115 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 8.1 nm
Dmax 30.0 nm
VolumePorod 430 nm3

SASDJ58 – Jagged1 high-affinity full ectodomain 11 µM

UniProt ID: Q9QXX0 (31-1067) Protein jagged-1
Protein jagged-1 experimental SAS data
Protein jagged-1 Kratky plot
Sample: Protein jagged-1, 115 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 8.9 nm
Dmax 33.0 nm
VolumePorod 505 nm3

SASDJ68 – Jagged1 high-affinity full ectodomain 21 µM

UniProt ID: Q9QXX0 (31-1067) Protein jagged-1
Protein jagged-1 experimental SAS data
Protein jagged-1 Kratky plot
Sample: Protein jagged-1, 115 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 9.6 nm
Dmax 42.0 nm
VolumePorod 715 nm3

SASDJ78 – Jagged1 high-affinity full ectodomain 42 µM

UniProt ID: Q9QXX0 (31-1067) Protein jagged-1
Protein jagged-1 experimental SAS data
Protein jagged-1 Kratky plot
Sample: Protein jagged-1, 115 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 10.2 nm
Dmax 43.0 nm
VolumePorod 900 nm3

SASDJ88 – Jagged1 EGF8-11 29 µM

UniProt ID: Q9QXX0 (487-665) Protein jagged-1 EGF8-11
Protein jagged-1 EGF8-11 experimental SAS data
Protein jagged-1 EGF8-11 Kratky plot
Sample: Protein jagged-1 EGF8-11, 21 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Dec 11
Notch-Jagged signaling complex defined by an interaction mosaic. Proc Natl Acad Sci U S A 118(30) (2021)
Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC
RgGuinier 3.3 nm
Dmax 11.0 nm
VolumePorod 26 nm3