SASBDB entries for UniProt ID:

SASDKV8 – ADAMTS-13 bound to von Willebrand Factor peptide

UniProt ID: P04275 (1596-1668) von Willebrand factor

UniProt ID: Q76LX8 (75-685) A disintegrin and metalloproteinase with thrombospondin motifs 13
von Willebrand factorA disintegrin and metalloproteinase with thrombospondin motifs 13 experimental SAS data
ADAMTS-13 bound to von Willebrand Factor peptide Rg histogram
Sample: Von Willebrand factor monomer, 11 kDa Homo sapiens protein
A disintegrin and metalloproteinase with thrombospondin motifs 13 monomer, 70 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 24
An Integrative Structural Biology Analysis of Von Willebrand Factor Binding and Processing by ADAMTS-13 in Solution. J Mol Biol 433(13):166954 (2021)
Del Amo-Maestro L, Sagar A, Pompach P, Goulas T, Scavenius C, Ferrero DS, Castrillo-Briceño M, Taulés M, Enghild JJ, Bernadó P, Gomis-Rüth FX
RgGuinier 4.4 nm
Dmax 16.3 nm
VolumePorod 170 nm3

SASDKW8 – Full-length pUL21 with C-terminal His-tag

UniProt ID: P10205 (1-535) Tegument protein UL21
Tegument protein UL21 experimental SAS data
Full-length pUL21 with C-terminal His-tag Rg histogram
Sample: Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread. PLoS Pathog 17(8):e1009824 (2021)
Benedyk TH, Muenzner J, Connor V, Han Y, Brown K, Wijesinghe KJ, Zhuang Y, Colaco S, Stoll GA, Tutt OS, Svobodova S, Svergun DI, Bryant NA, Deane JE, Firth AE, Jeffries CM, Crump CM, Graham SC
RgGuinier 4.2 nm
Dmax 18.1 nm
VolumePorod 90 nm3

SASDKX8 – Aedes aegypti Toll5A receptor ectodomain (T5A)

UniProt ID: A0A6I8TEX2 (28-789) Toll-like receptor
Toll-like receptor experimental SAS data
Toll-like receptor Kratky plot
Sample: Toll-like receptor dimer, 175 kDa Aedes aegypti protein
Buffer: 50 mM NaCl, 50 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Aug 2
Structure and dynamics of Toll immunoreceptor activation in the mosquito Aedes aegypti. Nat Commun 13(1):5110 (2022)
Saucereau Y, Wilson TH, Tang MCK, Moncrieffe MC, Hardwick SW, Chirgadze DY, Soares SG, Marcaida MJ, Gay NJ, Gangloff M
RgGuinier 5.4 nm
Dmax 20.5 nm
VolumePorod 370 nm3

SASDKY8 – Aedes aegypti Toll5A ectodomain in complex with Spz1C Cys-knot domain

UniProt ID: A0A6I8TEX2 (28-789) Toll-like receptor

UniProt ID: Q16J57 (142-243) AAEL013433-PA (Cystine-knot_cytokine)
Toll-like receptorAAEL013433-PA (Cystine-knot_cytokine) experimental SAS data
Toll-like receptor AAEL013433-PA (Cystine-knot_cytokine) Kratky plot
Sample: Toll-like receptor dimer, 175 kDa Aedes aegypti protein
AAEL013433-PA (Cystine-knot_cytokine) dimer, 26 kDa Aedes aegypti protein
Buffer: 50 mM NaCl, 50 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Aug 2
Structure and dynamics of Toll immunoreceptor activation in the mosquito Aedes aegypti. Nat Commun 13(1):5110 (2022)
Saucereau Y, Wilson TH, Tang MCK, Moncrieffe MC, Hardwick SW, Chirgadze DY, Soares SG, Marcaida MJ, Gay NJ, Gangloff M
RgGuinier 5.5 nm
Dmax 21.5 nm
VolumePorod 567 nm3

SASDKZ8 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 0.74 mg/ml

UniProt ID: Q15796 (1-467) Mothers against decapentaplegic homolog 2
Mothers against decapentaplegic homolog 2 experimental SAS data
Mothers against decapentaplegic homolog 2 Kratky plot
Sample: Mothers against decapentaplegic homolog 2, 160 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
RgGuinier 3.4 nm
Dmax 15.0 nm

SASDK29 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 1.3 mg/ml

UniProt ID: Q15796 (1-467) Mothers against decapentaplegic homolog 2
Mothers against decapentaplegic homolog 2 experimental SAS data
Mothers against decapentaplegic homolog 2 Kratky plot
Sample: Mothers against decapentaplegic homolog 2, 160 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
RgGuinier 3.8 nm
Dmax 21.0 nm

SASDK39 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 2.5 mg/ml

UniProt ID: Q15796 (1-467) Mothers against decapentaplegic homolog 2
Mothers against decapentaplegic homolog 2 experimental SAS data
Mothers against decapentaplegic homolog 2 Kratky plot
Sample: Mothers against decapentaplegic homolog 2, 160 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
RgGuinier 3.8 nm
Dmax 20.0 nm

SASDK49 – Mothers against decapentaplegic homolog 2, SMAD2, wild-type 3mg/ml

UniProt ID: Q15796 (1-467) Mothers against decapentaplegic homolog 2
Mothers against decapentaplegic homolog 2 experimental SAS data
Mothers against decapentaplegic homolog 2 Kratky plot
Sample: Mothers against decapentaplegic homolog 2, 160 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
RgGuinier 4.3 nm
Dmax 23.0 nm

SASDK59 – Mothers against decapentaplegic homolog 2, SMAD2, linker fragment (amino acids 175-262)

UniProt ID: Q15796 (175-262) Mothers against decapentaplegic homolog 2 (linker)
Mothers against decapentaplegic homolog 2 (linker) experimental SAS data
Mothers against decapentaplegic homolog 2 (linker) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (linker) monomer, 10 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
RgGuinier 2.9 nm
Dmax 14.0 nm
VolumePorod 20 nm3

SASDK69 – Mothers against decapentaplegic homolog 2, SMAD2, linker-MH2 fragment (amino acids 186-467) 0.5 mg/ml

UniProt ID: Q15796 (186-467) Mothers against decapentaplegic homolog 2 (linker-MH2)
Mothers against decapentaplegic homolog 2 (linker-MH2) experimental SAS data
Mothers against decapentaplegic homolog 2 (linker-MH2) Kratky plot
Sample: Mothers against decapentaplegic homolog 2 (linker-MH2), 99 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 11
Conformational landscape of multidomain SMAD proteins Computational and Structural Biotechnology Journal (2021)
Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M
RgGuinier 3.6 nm
Dmax 15.0 nm