SASBDB entries for UniProt ID:

SASDN55 – A recoverin homology domain with EF hand domain of diacylglycerol kinase alpha under the condition with CaCl2

UniProt ID: P23743 (1-197) Diacylglycerol kinase alpha
Diacylglycerol kinase alpha experimental SAS data
DAMMIF model
Sample: Diacylglycerol kinase alpha monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM TrisHCl, 200 mM NaCl, 5 mM DTT, 5% v/v glycerol, 3 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Dec 8
Ca 2+ ‐induced structural changes and intramolecular interactions in N‐terminal region of diacylglycerol kinase alpha Protein Science 31(7) (2022)
Takahashi D, Yonezawa K, Okizaki Y, Caaveiro J, Ueda T, Shimada A, Sakane F, Shimizu N
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 44 nm3

SASDN65 – A recoverin homology domain with EF hand domain of diacylglycerol kinase alpha under the condition without CaCl2

UniProt ID: P23743 (1-197) Diacylglycerol kinase alpha
Diacylglycerol kinase alpha experimental SAS data
DAMMIF model
Sample: Diacylglycerol kinase alpha monomer, 22 kDa Homo sapiens protein
Buffer: 20mM TrisHCl, 200mM NaCl, 5mM DTT, 5% v/v Glycerol, 3mM EGTA, pH: 7.4
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Dec 8
Ca 2+ ‐induced structural changes and intramolecular interactions in N‐terminal region of diacylglycerol kinase alpha Protein Science 31(7) (2022)
Takahashi D, Yonezawa K, Okizaki Y, Caaveiro J, Ueda T, Shimada A, Sakane F, Shimizu N
RgGuinier 1.9 nm
Dmax 7.0 nm
VolumePorod 44 nm3

SASDN75 – TetR/AcrR-like repressor SCO3201 from Streptomyces coelicor

UniProt ID: Q9KYU4 (23-236) Putative tetR-family transcriptional regulator
Putative tetR-family transcriptional regulator experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Putative tetR-family transcriptional regulator dimer, 47 kDa Streptomyces coelicolor (strain … protein
Buffer: 20 mM Tris/HCl, 400 mM NaCl, 50 mM imidazole, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2014 May 2
Crystal Structures of Free and Ligand-Bound Forms of the TetR/AcrR-Like Regulator SCO3201 from Streptomyces coelicolor Suggest a Novel Allosteric Mechanism. FEBS J (2022)
Werten S, Waack P, Palm GJ, Virolle MJ, Hinrichs W
RgGuinier 2.6 nm
Dmax 6.5 nm
VolumePorod 77 nm3

SASDN85 – Truncated Kelch13-Wild Type

UniProt ID: Q8IDQ2 (189-726) Kelch protein K13
Kelch protein K13 experimental SAS data
ITASSER model
Sample: Kelch protein K13 hexamer, 396 kDa Plasmodium falciparum (isolate … protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2020 Jul 25
Plasmodium falciparum Kelch13 and its artemisinin-resistant mutants assemble as hexamers in solution: a SAXS data driven modeling study. FEBS J (2022)
Goel N, Dhiman K, Kalidas N, Mukhopadhyay A, Ashish F, Bhattacharjee S
RgGuinier 6.4 nm
Dmax 17.0 nm
VolumePorod 1630 nm3

SASDN95 – Truncated Kelch13-R539T mutant

UniProt ID: Q8IDQ2 (189-726) Kelch protein K13 (Truncated Kelch13-R539T, artemisinin-resistant mutation)
Kelch protein K13 (Truncated Kelch13-R539T, artemisinin-resistant mutation) experimental SAS data
GASBOR model
Sample: Kelch protein K13 (Truncated Kelch13-R539T, artemisinin-resistant mutation) hexamer, 396 kDa Plasmodium falciparum (isolate … protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2020 Jul 25
Plasmodium falciparum Kelch13 and its artemisinin-resistant mutants assemble as hexamers in solution: a SAXS data driven modeling study. FEBS J (2022)
Goel N, Dhiman K, Kalidas N, Mukhopadhyay A, Ashish F, Bhattacharjee S
RgGuinier 6.6 nm
Dmax 20.5 nm
VolumePorod 1600 nm3

SASDNA5 – Truncated Kelch13-C580Y mutant

UniProt ID: Q8IDQ2 (189-726) Kelch protein K13 (Truncated Kelch13-C580Y )
Kelch protein K13 (Truncated Kelch13-C580Y ) experimental SAS data
GASBOR model
Sample: Kelch protein K13 (Truncated Kelch13-C580Y ) hexamer, 397 kDa Plasmodium falciparum (isolate … protein
Buffer: Phosphate Buffer Saline, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2020 Jul 28
Plasmodium falciparum Kelch13 and its artemisinin-resistant mutants assemble as hexamers in solution: a SAXS data driven modeling study. FEBS J (2022)
Goel N, Dhiman K, Kalidas N, Mukhopadhyay A, Ashish F, Bhattacharjee S
RgGuinier 6.4 nm
Dmax 18.4 nm
VolumePorod 1800 nm3

SASDNB5 – Truncated Kelch13-A578S mutant

UniProt ID: Q8IDQ2 (189-726) Kelch protein K13 (Truncated Kelch13-A578S)
Kelch protein K13 (Truncated Kelch13-A578S) experimental SAS data
GASBOR model
Sample: Kelch protein K13 (Truncated Kelch13-A578S) hexamer, 396 kDa Plasmodium falciparum (isolate … protein
Buffer: Phosphate Buffer Saline, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2020 Jul 29
Plasmodium falciparum Kelch13 and its artemisinin-resistant mutants assemble as hexamers in solution: a SAXS data driven modeling study. FEBS J (2022)
Goel N, Dhiman K, Kalidas N, Mukhopadhyay A, Ashish F, Bhattacharjee S
RgGuinier 6.1 nm
Dmax 16.8 nm
VolumePorod 1700 nm3

SASDNC5 – Acinetobacter baumannii riboflavin biosynthesis protein RibD

UniProt ID: A0A5P1UGY3 (1-361) Riboflavin biosynthesis protein RibD
Riboflavin biosynthesis protein RibD experimental SAS data
GASBOR model
Sample: Riboflavin biosynthesis protein RibD dimer, 80 kDa Acinetobacter baumannii protein
Buffer: 150 mM NaCl, 10 mM Tris, 1 mM DTT, 5% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3
Recombinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA. Protein Expr Purif :106134 (2022)
Weber K, Doellinger J, Jeffries CM, Wilharm G
RgGuinier 3.7 nm
Dmax 12.0 nm
VolumePorod 115 nm3

SASDND5 – Acinetobacter baumannii aconitate hydratase B, monomer fraction

UniProt ID: V5VBJ4 (1-879) Aconitate hydratase B
Aconitate hydratase B experimental SAS data
GASBOR model
Sample: Aconitate hydratase B monomer, 96 kDa Acinetobacter baumannii protein
Buffer: 100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3
Recombinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA. Protein Expr Purif :106134 (2022)
Weber K, Doellinger J, Jeffries CM, Wilharm G
RgGuinier 3.4 nm
Dmax 12.5 nm
VolumePorod 150 nm3

SASDNE5 – Acinetobacter baumannii aconitate hydratase B, monomer/dimer mixed fraction

UniProt ID: V5VBJ4 (1-879) Aconitate hydratase B
Aconitate hydratase B experimental SAS data
GASBOR model
Sample: Aconitate hydratase B, 96 kDa Acinetobacter baumannii protein
Buffer: 100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3
Recombinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA. Protein Expr Purif :106134 (2022)
Weber K, Doellinger J, Jeffries CM, Wilharm G
RgGuinier 4.0 nm
Dmax 14.0 nm
VolumePorod 230 nm3