SASBDB entries for UniProt ID:

SASDNF5 – Acinetobacter baumannii transcriptional repressor NrdR (likely filamentous assembly)

UniProt ID: A3M1A1 (1-152) Transcriptional repressor NrdR
Transcriptional repressor NrdR experimental SAS data
Transcriptional repressor NrdR Kratky plot
Sample: Transcriptional repressor NrdR, 19 kDa Acinetobacter baumannii (strain … protein
Buffer: 100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3
Recombinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA. Protein Expr Purif :106134 (2022)
Weber K, Doellinger J, Jeffries CM, Wilharm G
RgGuinier 19.9 nm
Dmax 92.6 nm

SASDNG5 – Desulfofustis sp. PB-SRB1 ligand-gated ion channel with calcium measured by paused-flow SEC-SANS

UniProt ID: V4JF97 (34-642) Neurotransmitter-gated ion-channel ligand-binding domain-containing protein
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein experimental SAS data
GROMACS model
Sample: Neurotransmitter-gated ion-channel ligand-binding domain-containing protein pentamer, 342 kDa Desulfofustis sp. PB-SRB1 protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.5 mM deuterated n-Dodecyl-B-D-Maltoside, in D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 23
Biophysical characterization of calcium-binding and modulatory-domain dynamics in a pentameric ligand-gated ion channel. Proc Natl Acad Sci U S A 119(50):e2210669119 (2022)
Lycksell M, Rovšnik U, Hanke A, Martel A, Howard RJ, Lindahl E
RgGuinier 5.2 nm
Dmax 16.8 nm
VolumePorod 530 nm3

SASDNH5 – Desulfofustis sp. PB-SRB1 ligand-gated ion channel without calcium measured by paused-flow SEC-SANS

UniProt ID: V4JF97 (34-642) Neurotransmitter-gated ion-channel ligand-binding domain-containing protein
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein experimental SAS data
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Kratky plot
Sample: Neurotransmitter-gated ion-channel ligand-binding domain-containing protein pentamer, 342 kDa Desulfofustis sp. PB-SRB1 protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM EDTA, 0.5 mM deuterated n-Dodecyl-B-D-Maltoside, in D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 23
Biophysical characterization of calcium-binding and modulatory-domain dynamics in a pentameric ligand-gated ion channel. Proc Natl Acad Sci U S A 119(50):e2210669119 (2022)
Lycksell M, Rovšnik U, Hanke A, Martel A, Howard RJ, Lindahl E
RgGuinier 5.2 nm
Dmax 16.7 nm
VolumePorod 540 nm3

SASDNJ5 – Full length 3-phosphoinositide-dependent protein kinase (PDK1)

UniProt ID: O15530 (2-556) 3-phosphoinositide-dependent protein kinase 1
3-phosphoinositide-dependent protein kinase 1 experimental SAS data
DAMMIF model
Sample: 3-phosphoinositide-dependent protein kinase 1 monomer, 65 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
Sacerdoti M, Gross L, Riley A, Zehnder K, Ghode A, Klinke S, Anand G, Paris K, Winkel A, Herbrand A, Godage H, Cozier G, Süß E, Schulze J, Pastor-Flores D, Bollini M, Cappellari M, Svergun D, Gräwert M, Aramendia P, Leroux A, Potter B, Camacho C, Biondi R
RgGuinier 3.5 nm
Dmax 11.2 nm
VolumePorod 107 nm3

SASDNK5 – Full length 3-phosphoinositide-dependent protein kinase (PDK1) in the presence of HYG8 (2-O-benzoyl-Ins(1,3,4,5,6)P5)

UniProt ID: O15530 (2-556) 3-phosphoinositide-dependent protein kinase 1

UniProt ID: None (None-None) 2-O-benzoyl-Ins(1,3,4,5,6)P5
3-phosphoinositide-dependent protein kinase 12-O-benzoyl-Ins(1,3,4,5,6)P5 experimental SAS data
DAMMIF model
Sample: 3-phosphoinositide-dependent protein kinase 1 monomer, 65 kDa Homo sapiens protein
2-O-benzoyl-Ins(1,3,4,5,6)P5 monomer, 1 kDa synthetic construct
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, 1 μM HYG8, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
Sacerdoti M, Gross L, Riley A, Zehnder K, Ghode A, Klinke S, Anand G, Paris K, Winkel A, Herbrand A, Godage H, Cozier G, Süß E, Schulze J, Pastor-Flores D, Bollini M, Cappellari M, Svergun D, Gräwert M, Aramendia P, Leroux A, Potter B, Camacho C, Biondi R
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 98 nm3

SASDNL5 – Double mutant catalytic domain of 3-phosphoinositide-dependent protein kinase 1 (PDK1 50–359; Y188G Q292A)

UniProt ID: O15530 (50-359) 3-phosphoinositide-dependent protein kinase 1 (Y188G Q292A)
3-phosphoinositide-dependent protein kinase 1 (Y188G Q292A) experimental SAS data
SREFLEX model
Sample: 3-phosphoinositide-dependent protein kinase 1 (Y188G Q292A) monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
Sacerdoti M, Gross L, Riley A, Zehnder K, Ghode A, Klinke S, Anand G, Paris K, Winkel A, Herbrand A, Godage H, Cozier G, Süß E, Schulze J, Pastor-Flores D, Bollini M, Cappellari M, Svergun D, Gräwert M, Aramendia P, Leroux A, Potter B, Camacho C, Biondi R
RgGuinier 2.4 nm
Dmax 7.0 nm
VolumePorod 57 nm3

SASDNM5 – Skp-like protein, SKP

UniProt ID: Q9HXY5 (23-168) Skp-like protein
Skp-like protein experimental SAS data
Skp-like protein, SKP Rg histogram
Sample: Skp-like protein trimer, 55 kDa Pseudomonas aeruginosa (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, 10 % glycerol, pH: 8
Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2021 Feb 26
The periplasmic chaperone Skp prevents misfolding of the secretory lipase A from Pseudomonas aeruginosa Frontiers in Molecular Biosciences 9 (2022)
Papadopoulos A, Busch M, Reiners J, Hachani E, Baeumers M, Berger J, Schmitt L, Jaeger K, Kovacic F, Smits S, Kedrov A
RgGuinier 3.5 nm
Dmax 11.1 nm
VolumePorod 172 nm3

SASDNN5 – Meiosis protein TEX12 (Testis-expressed protein 12) – wild-type

UniProt ID: Q9BXU0 (49-123) Testis-expressed protein 12
Testis-expressed protein 12 experimental SAS data
DAMMIF model
Sample: Testis-expressed protein 12 dimer, 18 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jan 10
Coiled-coil structure of meiosis protein TEX12 and conformational regulation by its C-terminal tip Communications Biology 5(1) (2022)
Dunce J, Salmon L, Davies O
RgGuinier 2.1 nm
Dmax 6.6 nm
VolumePorod 27 nm3

SASDNP5 – Meiosis protein TEX12 (Testis-expressed protein 12) – delta Ctip

UniProt ID: Q9BXU0 (49-113) Testis-expressed protein 12
Testis-expressed protein 12 experimental SAS data
DAMMIF model
Sample: Testis-expressed protein 12 tetramer, 32 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jan 10
Coiled-coil structure of meiosis protein TEX12 and conformational regulation by its C-terminal tip Communications Biology 5(1) (2022)
Dunce J, Salmon L, Davies O
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 44 nm3

SASDNQ5 – Meiosis protein TEX12 (Testis-expressed protein 12) – L110E, F114E, I117E, L121E mutant

UniProt ID: Q9BXU0 (49-123) Testis-expressed protein 12 (L110E, F114E, I117E, L121E)
Testis-expressed protein 12 (L110E, F114E, I117E, L121E) experimental SAS data
DAMMIF model
Sample: Testis-expressed protein 12 (L110E, F114E, I117E, L121E) tetramer, 36 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Sep 15
Coiled-coil structure of meiosis protein TEX12 and conformational regulation by its C-terminal tip Communications Biology 5(1) (2022)
Dunce J, Salmon L, Davies O
RgGuinier 3.3 nm
Dmax 12.9 nm
VolumePorod 59 nm3