SASBDB entries for UniProt ID:

SASDNQ3 – Recombinant phospholipase A2 receptor at pH 6.2

UniProt ID: Q13018 (21-1397) Secretory phospholipase A2 receptor
Secretory phospholipase A2 receptor experimental SAS data
MODELLER model
Sample: Secretory phospholipase A2 receptor monomer, 189 kDa Homo sapiens protein
Buffer: 10 mM Bis-Tris, 150 mM NaCl, pH: 6.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 19
Structure of PLA2R reveals presentation of the dominant membranous nephropathy epitope and an immunogenic patch Proceedings of the National Academy of Sciences 119(29) (2022)
Fresquet M, Lockhart-Cairns M, Rhoden S, Jowitt T, Briggs D, Baldock C, Brenchley P, Lennon R
RgGuinier 4.2 nm
Dmax 15.8 nm
VolumePorod 426 nm3

SASDNR3 – Fibrillin-1 fragment PF3

UniProt ID: P35555 (1-722) Fibrillin-1 PF3
Fibrillin-1 PF3 experimental SAS data
DAMMIF model
Sample: Fibrillin-1 PF3 monomer, 78 kDa Homo sapiens protein
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Dec 12
Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biol (2022)
Lockhart-Cairns MP, Cain SA, Dajani R, Steer R, Thomson J, Alanazi YF, Kielty CM, Baldock C
RgGuinier 5.4 nm
Dmax 24.0 nm
VolumePorod 141 nm3

SASDNS3 – Latent-transforming growth factor beta-binding protein 1 C-terminus

UniProt ID: Q14766-2 (1329-1721) Latent-transforming growth factor beta-binding protein 1
Latent-transforming growth factor beta-binding protein 1 experimental SAS data
DAMMIF model
Sample: Latent-transforming growth factor beta-binding protein 1 monomer, 44 kDa Homo sapiens protein
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Dec 12
Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biol (2022)
Lockhart-Cairns MP, Cain SA, Dajani R, Steer R, Thomson J, Alanazi YF, Kielty CM, Baldock C
RgGuinier 4.7 nm
Dmax 20.5 nm
VolumePorod 102 nm3

SASDNT3 – Fibrillin-1 fragment PF3 crosslinked to latent transforming growth factor-beta-binding protein 1 C-terminus by transglutaminase 2

UniProt ID: Q14766-2 (1329-1721) Latent-transforming growth factor beta-binding protein 1

UniProt ID: P35555 (1-722) Fibrillin-1 PF3
Latent-transforming growth factor beta-binding protein 1Fibrillin-1 PF3 experimental SAS data
DAMMIF model
Sample: Latent-transforming growth factor beta-binding protein 1 monomer, 44 kDa Homo sapiens protein
Fibrillin-1 PF3 monomer, 78 kDa Homo sapiens protein
Buffer: 10 mM Hepes, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 20
Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biol (2022)
Lockhart-Cairns MP, Cain SA, Dajani R, Steer R, Thomson J, Alanazi YF, Kielty CM, Baldock C
RgGuinier 7.4 nm
Dmax 29.6 nm
VolumePorod 759 nm3

SASDNU3 – Fibrillin PF2

UniProt ID: P35555 (330-722) Fibrillin-1
Fibrillin-1 experimental SAS data
DAMMIF model
Sample: Fibrillin-1 monomer, 44 kDa Homo sapiens protein
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at ID02, ESRF on 2006 Feb 11
Transglutaminase-Mediated Cross-Linking of Tropoelastin to Fibrillin Stabilises the Elastin Precursor Prior to Elastic Fibre Assembly. J Mol Biol 432(21):5736-5751 (2020)
Lockhart-Cairns MP, Newandee H, Thomson J, Weiss AS, Baldock C, Tarakanova A
RgGuinier 4.3 nm
Dmax 15.0 nm
VolumePorod 84 nm3

SASDNV3 – Synthetic Human Elastin without domain 26A

UniProt ID: P15502-2 (27-724) Elastin
Elastin experimental SAS data
Elastin Kratky plot
Sample: Elastin monomer, 60 kDa Homo sapiens protein
Buffer: Phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at ID02, ESRF on 2011 Mar 15
Transglutaminase-Mediated Cross-Linking of Tropoelastin to Fibrillin Stabilises the Elastin Precursor Prior to Elastic Fibre Assembly. J Mol Biol 432(21):5736-5751 (2020)
Lockhart-Cairns MP, Newandee H, Thomson J, Weiss AS, Baldock C, Tarakanova A
RgGuinier 6.6 nm
Dmax 22.5 nm

SASDNW3 – Fibrillin-1 fragment PF2 crosslinked to tropoelastin by transglutaminase 2

UniProt ID: P35555 (330-722) Fibrillin-1

UniProt ID: P15502 (27-724) Elastin
Fibrillin-1Elastin experimental SAS data
DAMMIF model
Sample: Fibrillin-1 monomer, 44 kDa Homo sapiens protein
Elastin monomer, 60 kDa Homo sapiens protein
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Feb 21
Transglutaminase-Mediated Cross-Linking of Tropoelastin to Fibrillin Stabilises the Elastin Precursor Prior to Elastic Fibre Assembly. J Mol Biol 432(21):5736-5751 (2020)
Lockhart-Cairns MP, Newandee H, Thomson J, Weiss AS, Baldock C, Tarakanova A
RgGuinier 7.1 nm
Dmax 23.5 nm

SASDNX3 – Calcium-bound Calmodulin, including structural models

UniProt ID: P0DP23 (2-149) Calmodulin-1
Calmodulin-1 experimental SAS data
Calcium-bound Calmodulin, including structural models Rg histogram
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Nov 15
Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium. BMC Biol 20(1):176 (2022)
Léger C, Pitard I, Sadi M, Carvalho N, Brier S, Mechaly A, Raoux-Barbot D, Davi M, Hoos S, Weber P, Vachette P, Durand D, Haouz A, Guijarro JI, Ladant D, Chenal A
RgGuinier 2.2 nm
Dmax 7.2 nm
VolumePorod 33 nm3

SASDNY3 – Calcium-bound Calmodulin complexed with Calmidazolium

UniProt ID: P0DP23 (2-149) Calmodulin-1

UniProt ID: None (None-None) Calmidazolium
Calmodulin-1Calmidazolium experimental SAS data
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Calmidazolium monomer, 1 kDa
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Nov 15
Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium. BMC Biol 20(1):176 (2022)
Léger C, Pitard I, Sadi M, Carvalho N, Brier S, Mechaly A, Raoux-Barbot D, Davi M, Hoos S, Weber P, Vachette P, Durand D, Haouz A, Guijarro JI, Ladant D, Chenal A
RgGuinier 1.7 nm
Dmax 5.2 nm
VolumePorod 30 nm3

SASDNZ3 – Heterodimerization between the nuclear receptor subfamily proteins PXR and CAR

UniProt ID: O75469 (130-434) Nuclear receptor subfamily 1 group I member 2

UniProt ID: Q14994 (103-352) Nuclear receptor subfamily 1 group I member 3
Nuclear receptor subfamily 1 group I member 2Nuclear receptor subfamily 1 group I member 3 experimental SAS data
DAMMIF model
Sample: Nuclear receptor subfamily 1 group I member 2 monomer, 39 kDa Homo sapiens protein
Nuclear receptor subfamily 1 group I member 3 monomer, 32 kDa Homo sapiens protein
Buffer: 25 mM Hepes, 150 mM NaCl, 5% glycerol, 5 mM DTT, pH: 7.9
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2021 Oct 8
Molecular basis of crosstalk in nuclear receptors: heterodimerization between PXR and CAR and the implication in gene regulation
Shirish chodankar
RgGuinier 2.7 nm
Dmax 10.1 nm
VolumePorod 56 nm3