SASBDB entries for UniProt ID:

SASDN97 – Human thryoglobulin measured using SEC-SAXS

UniProt ID: P01266 (1-2768) Thyroglobulin
Thyroglobulin experimental SAS data
DAMMIN model
Sample: Thyroglobulin dimer, 610 kDa Homo sapiens protein
Buffer: phosphate buffered saline, 150mM NaCl, pH: 8
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2020 Jul 17
Thyroglobulin - human and bovine SAXS/WAXS data
James Byrnes
RgGuinier 7.7 nm
Dmax 26.3 nm
VolumePorod 1800 nm3

SASDNA7 – Bovine thyroglobulin in phosphate buffered saline/NaCl

UniProt ID: P01267 (1-2769) Thyroglobulin
Thyroglobulin experimental SAS data
DAMMIF model
Sample: Thyroglobulin dimer, 606 kDa Bos taurus protein
Buffer: phosphate buffered saline, 150mM NaCl, pH: 8
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2020 Jul 17
Thyroglobulin - human and bovine SAXS/WAXS data
James Byrnes
RgGuinier 8.1 nm
Dmax 29.8 nm
VolumePorod 1950 nm3

SASDNB7 – C-terminal domain of pUL21 with N-terminal His-tag

UniProt ID: P10205 (275-535) Tegument protein UL21 (C-terminal domain)
Tegument protein UL21 (C-terminal domain) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 2.2 nm
Dmax 8.5 nm
VolumePorod 49 nm3

SASDNC7 – miniCERTL with N-terminal His-tag

UniProt ID: Q9Y5P4 (None-None) Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624)
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 2.7 nm
Dmax 9.1 nm
VolumePorod 71 nm3

SASDND7 – Complex of H6-pUL21C and H6-miniCERTL

UniProt ID: P10205 (275-535) Tegument protein UL21 (C-terminal domain)

UniProt ID: Q9Y5P4 (None-None) Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624)
Tegument protein UL21 (C-terminal domain)Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 3.3 nm
Dmax 13.6 nm
VolumePorod 84 nm3

SASDNE7 – Complex of H6-miniCERTL and full-length pUL21-H6

UniProt ID: P10205 (1-535) Tegument protein UL21

UniProt ID: Q9Y5P4 (None-None) Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624)
Tegument protein UL21Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 4.1 nm
Dmax 15.8 nm
VolumePorod 158 nm3

SASDNF7 – CK2α (Casein kinase II subunit alpha)

UniProt ID: P68400 (3-330) Casein kinase II subunit alpha
Casein kinase II subunit alpha experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Casein kinase II subunit alpha monomer, 39 kDa Homo sapiens protein
Buffer: 25 mM Tris, 500 mM NaCl, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Oct 10
Mechanism of CK2 Inhibition by a Ruthenium-Based Polyoxometalate. Front Mol Biosci 9:906390 (2022)
Fabbian S, Giachin G, Bellanda M, Borgo C, Ruzzene M, Spuri G, Campofelice A, Veneziano L, Bonchio M, Carraro M, Battistutta R
RgGuinier 2.2 nm
Dmax 6.7 nm
VolumePorod 59 nm3

SASDNG7 – CK2α (Casein kinase II subunit alpha) dimer bound to ruthenium-based polyoxometalate (Ru4POM)

UniProt ID: P68400 (3-330) Casein kinase II subunit alpha
Casein kinase II subunit alpha experimental SAS data
CUSTOM IN-HOUSE model
Sample: Casein kinase II subunit alpha dimer, 78 kDa Homo sapiens protein
Buffer: 25 mM Tris, 500 mM NaCl, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2021 Oct 10
Mechanism of CK2 Inhibition by a Ruthenium-Based Polyoxometalate. Front Mol Biosci 9:906390 (2022)
Fabbian S, Giachin G, Bellanda M, Borgo C, Ruzzene M, Spuri G, Campofelice A, Veneziano L, Bonchio M, Carraro M, Battistutta R
RgGuinier 3.0 nm
Dmax 11.8 nm
VolumePorod 122 nm3

SASDNH7 – Tn3 resolvase dimer bound to site II DNA sequence

UniProt ID: P0ADI2 (1-185) Transposon Tn3 resolvase

UniProt ID: None (None-None) Tn3 res, resolvase binding site II
Transposon Tn3 resolvaseTn3 res, resolvase binding site II experimental SAS data
CUSTOM IN-HOUSE model
Sample: Transposon Tn3 resolvase dimer, 43 kDa Escherichia coli protein
Tn3 res, resolvase binding site II monomer, 32 kDa DNA
Buffer: 25 mM Tris, 150 mM NaCl, 10 mM MgCl2, 1% glycerol, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 6
Structural basis for topological regulation of Tn3 resolvase. Nucleic Acids Res (2022)
Montaño SP, Rowland SJ, Fuller JR, Burke ME, MacDonald AI, Boocock MR, Stark WM, Rice PA
RgGuinier 5.0 nm
Dmax 14.9 nm
VolumePorod 217 nm3

SASDNM7 – Protease 1 from Pyrococcus horikoshii (PhP1) in Gd-HPDO3A

UniProt ID: O59413 (1-166) Deglycase PH1704
Deglycase PH1704 experimental SAS data
OTHER model
Sample: Deglycase PH1704 hexamer, 112 kDa Pyrococcus horikoshii (strain … protein
Buffer: 20 mM Tris pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Jun 7
Medical contrast agents as promising tools for biomacromolecular SAXS experiments. Acta Crystallogr D Struct Biol 78(Pt 9):1120-1130 (2022)
Gabel F, Engilberge S, Schmitt E, Thureau A, Mechulam Y, Pérez J, Girard E
RgGuinier 3.2 nm
Dmax 9.2 nm
VolumePorod 140 nm3