SASBDB entries for UniProt ID:

SASDNB8 – Mature full length human prion protein

UniProt ID: P04156 (23-231) Major prion protein
Major prion protein experimental SAS data
Mature full length human prion protein Rg histogram
Sample: Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer: 10 mM HEPES 100 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 13
A tetracationic porphyrin with dual anti-prion activity. iScience 26(9):107480 (2023)
Masone A, Zucchelli C, Caruso E, Lavigna G, Eraña H, Giachin G, Tapella L, Comerio L, Restelli E, Raimondi I, Elezgarai SR, De Leo F, Quilici G, Taiarol L, Oldrati M, Lorenzo NL, García-Martínez S, Cagnotto A, Lucchetti J, Gobbi M, Vanni I, Nonno R, Di Bari MA, Tully MD, Cecatiello V, Ciossani G, Pasqualato S, Van Anken E, Salmona M, Castilla J, Requena JR, Banfi S, Musco G, Chiesa R
RgGuinier 2.8 nm
Dmax 10.2 nm

SASDNC8 – Mature full length human prion protein in the presence of zinc-tetracationic porphyrin [Zn(II)-BnPyP]

UniProt ID: P04156 (23-231) Major prion protein
Major prion protein experimental SAS data
Mature full length human prion protein in the presence of zinc-tetracationic porphyrin [Zn(II)-BnPyP] Rg histogram
Sample: Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer: 10 mM HEPES 100 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 13
A tetracationic porphyrin with dual anti-prion activity. iScience 26(9):107480 (2023)
Masone A, Zucchelli C, Caruso E, Lavigna G, Eraña H, Giachin G, Tapella L, Comerio L, Restelli E, Raimondi I, Elezgarai SR, De Leo F, Quilici G, Taiarol L, Oldrati M, Lorenzo NL, García-Martínez S, Cagnotto A, Lucchetti J, Gobbi M, Vanni I, Nonno R, Di Bari MA, Tully MD, Cecatiello V, Ciossani G, Pasqualato S, Van Anken E, Salmona M, Castilla J, Requena JR, Banfi S, Musco G, Chiesa R
RgGuinier 3.1 nm
Dmax 11.3 nm

SASDND8 – Mature full length human prion protein in the presence of a cationic Fe(III) porphyrin [Fe(III)-TMPyP]

UniProt ID: P04156 (23-231) Major prion protein
Major prion protein experimental SAS data
Mature full length human prion protein in the presence of a cationic Fe(III) porphyrin [Fe(III)-TMPyP] Rg histogram
Sample: Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer: 10 mM HEPES 100 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 13
A tetracationic porphyrin with dual anti-prion activity. iScience 26(9):107480 (2023)
Masone A, Zucchelli C, Caruso E, Lavigna G, Eraña H, Giachin G, Tapella L, Comerio L, Restelli E, Raimondi I, Elezgarai SR, De Leo F, Quilici G, Taiarol L, Oldrati M, Lorenzo NL, García-Martínez S, Cagnotto A, Lucchetti J, Gobbi M, Vanni I, Nonno R, Di Bari MA, Tully MD, Cecatiello V, Ciossani G, Pasqualato S, Van Anken E, Salmona M, Castilla J, Requena JR, Banfi S, Musco G, Chiesa R
RgGuinier 2.8 nm
Dmax 10.3 nm

SASDNF8 – LARGE xylosyl- and glucuronyltransferase 1 (LARGE1dTM) dimer

UniProt ID: O95461 (34-756) Xylosyl- and glucuronyltransferase LARGE1
Xylosyl- and glucuronyltransferase LARGE1 experimental SAS data
DAMMIF model
Sample: Xylosyl- and glucuronyltransferase LARGE1 dimer, 179 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
Joseph S, Schnicker N, Xu Z, Yang T, Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.4 nm
Dmax 18.4 nm
VolumePorod 264 nm3

SASDNG8 – LARGE xylosyl- and glucuronyltransferase 1 (LARGE1dTM) dimer treated with PNGase F enzyme

UniProt ID: O95461 (34-756) Xylosyl- and glucuronyltransferase LARGE1
Xylosyl- and glucuronyltransferase LARGE1 experimental SAS data
DAMMIF model
Sample: Xylosyl- and glucuronyltransferase LARGE1 dimer, 179 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
Joseph S, Schnicker N, Xu Z, Yang T, Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.3 nm
Dmax 18.0 nm

SASDNH8 – Xylosyl- and glucuronyltransferase LARGE2 (LARGE2dTM) dimer

UniProt ID: Q5XPT3 (30-690) Xylosyl- and glucuronyltransferase LARGE2
Xylosyl- and glucuronyltransferase LARGE2 experimental SAS data
DAMMIN model
Sample: Xylosyl- and glucuronyltransferase LARGE2 dimer, 168 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
Joseph S, Schnicker N, Xu Z, Yang T, Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.2 nm
Dmax 16.5 nm

SASDNJ8 – Xylosyl- and glucuronyltransferase LARGE2 (LARGE2dTM) dimer treated with PNGase F enzyme

UniProt ID: Q5XPT3 (30-690) Xylosyl- and glucuronyltransferase LARGE2
Xylosyl- and glucuronyltransferase LARGE2 experimental SAS data
DAMMIN model
Sample: Xylosyl- and glucuronyltransferase LARGE2 dimer, 168 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
Joseph S, Schnicker N, Xu Z, Yang T, Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.1 nm
Dmax 15.9 nm
VolumePorod 231 nm3

SASDNM8 – Quorum sensing master protein (QseM)

UniProt ID: Q8KGS3 (2-83) DUF2285 domain-containing protein
DUF2285 domain-containing protein experimental SAS data
DUF2285 domain-containing protein Kratky plot
Sample: DUF2285 domain-containing protein monomer, 11 kDa Mesorhizobium loti protein
Buffer: 100 mM NaCl, 50 mM Tris-HCl, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 1
DUF2285 is a novel helix-turn-helix domain variant that orchestrates both activation and antiactivation of conjugative element transfer in proteobacteria. Nucleic Acids Res (2023)
Jowsey WJ, Morris CRP, Hall DA, Sullivan JT, Fagerlund RD, Eto KY, Solomon PD, Mackay JP, Bond CS, Ramsay JP, Ronson CW
RgGuinier 1.5 nm
Dmax 5.7 nm
VolumePorod 15 nm3

SASDNN8 – Upstream of N-ras, isoform A, CSD 7, 8 and 9 from Drosophila melanogaster in complex with a poly(A)-15mer RNA

UniProt ID: Q9VSK3 (757-991) Upstream of N-ras, isoform A

UniProt ID: None (None-None) polyA-15mer
Upstream of N-ras, isoform ApolyA-15mer experimental SAS data
Upstream of N-ras, isoform A polyA-15mer Kratky plot
Sample: Upstream of N-ras, isoform A monomer, 26 kDa Drosophila melanogaster protein
PolyA-15mer monomer, 5 kDa RNA
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 8
Upstream of N-Ras C-terminal cold shock domains mediate poly(A) specificity in a novel RNA recognition mode and bind poly(A) binding protein. Nucleic Acids Res (2023)
Hollmann NM, Jagtap PKA, Linse JB, Ullmann P, Payr M, Murciano B, Simon B, Hub JS, Hennig J
RgGuinier 2.2 nm
Dmax 7.5 nm
VolumePorod 37 nm3

SASDNP8 – High mannose contactin-2 immunoglobulin domains1-6, 1μM

UniProt ID: Q61330 (29-608) Contactin-2
Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.1 nm
Dmax 20.0 nm
VolumePorod 140 nm3