Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDP56 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 250 mM NaCl buffer

UniProt ID: P0DTC1 (3943-4140) Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2)

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J

R_g^Guinier

4.1

SASDP66 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 500 mM NaCl buffer

UniProt ID: P0DTC1 (3943-4140) Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2)

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	3.8	nm
D_max	14.0	nm
Volume^Porod	98	nm³

SASDP76 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 1000 mM NaCl buffer

UniProt ID: P0DTC1 (3943-4140) Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2)

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	3.8	nm
D_max	14.0	nm
Volume^Porod	100	nm³

SASDP86 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 250 mM NaCl buffer

UniProt ID: P0DTC1 (None-None) Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2)

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	6.3	nm
D_max	24.0	nm

SASDP96 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 500 mM NaCl buffer

UniProt ID: P0DTC1 (None-None) Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2)

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	5.2	nm
D_max	18.0	nm

SASDPA6 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 1000 mM NaCl buffer

UniProt ID: P0DTC1 (None-None) Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2)

Sample:	Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	4.8	nm
D_max	16.0	nm

SASDPB6 – Fibronectin 2 and 3 of cell adhesion molecule L1 (L1CAM) expressed in HEK293S GnTI-

UniProt ID: P32004 (712-917) Neural cell adhesion molecule L1

Neural cell adhesion molecule L1 experimental SAS data

Sample:	Neural cell adhesion molecule L1 monomer, 24 kDa Homo sapiens protein
Buffer:	50 mM MES pH 6.5, 150 mM NaCl, 5% glycerol, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 May 11

X‐ray structure and function of fibronectin domains two and three of the neural cell adhesion molecule L1 The FASEB Journal 37(3) (2023)
Guédez G, Loers G, Jeffries C, Kozak S, Meijers R, Svergun D, Schachner M, Löw C

R_g^Guinier	2.8	nm
D_max	9.5	nm
Volume^Porod	68	nm³

SASDPC6 – Fibronectin 2 and 3 of cell adhesion molecule L1 (L1CAM) expressed in HEK293F

UniProt ID: P32004 (712-917) Neural cell adhesion molecule L1

Sample:	Neural cell adhesion molecule L1 monomer, 24 kDa Homo sapiens protein
Buffer:	50 mM MES pH 6.5, 150 mM NaCl, 5% glycerol, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 May 11

R_g^Guinier	2.9	nm
D_max	9.9	nm
Volume^Porod	72	nm³

SASDPD6 – Fibronectin 2 and 3 of cell adhesion molecule L1 (L1CAM) expressed in HEK293F cells with kifunensine

UniProt ID: P32004 (712-917) Neural cell adhesion molecule L1

Sample:	Neural cell adhesion molecule L1 monomer, 24 kDa Homo sapiens protein
Buffer:	50 mM MES pH 6.5, 150 mM NaCl, 5% glycerol, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 May 11

R_g^Guinier	2.9	nm
D_max	9.6	nm
Volume^Porod	72	nm³

SASDPV6 – Calmodulin

UniProt ID: P0DP23 (1-149) Calmodulin-1

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23

Binding by calmodulin is coupled to transient unfolding of the third FF domain of Prp40A. Protein Sci 32(4):e4606 (2023)
Díaz Casas A, Cordoba JJ, Ferrer BJ, Balakrishnan S, Wurm JE, Pastrana-Ríos B, Chazin WJ

R_g^Guinier	2.2	nm
D_max	7.0	nm
Volume^Porod	38	nm³

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