SASBDB entries for UniProt ID:

SASDP39 – apo Saccharomyces cerevisiae Arginyltransferase-1

UniProt ID: P16639 (1-503) Arginyl-tRNA--protein transferase 1
Arginyl-tRNA--protein transferase 1 experimental SAS data
ALPHAFOLD model
Sample: Arginyl-tRNA--protein transferase 1 monomer, 60 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 2% glycerol, 100 mM KCl, 50 mM Tris-HCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 12
The Structure of Saccharomyces cerevisiae Arginyltransferase 1 (ATE1). J Mol Biol 434(21):167816 (2022)
Van V, Ejimogu NE, Bui TS, Smith AT
RgGuinier 2.8 nm
Dmax 8.3 nm
VolumePorod 105 nm3

SASDP69 – Acinetobacter baumannii carbonic anhydrase PaaY trimer

UniProt ID: D0C8J4 (1-201) Bacterial transferase hexapeptide repeat protein
Bacterial transferase hexapeptide repeat protein experimental SAS data
CORAL model
Sample: Bacterial transferase hexapeptide repeat protein trimer, 66 kDa Acinetobacter baumannii (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jan 29
Mechanistic and structural insights into the bifunctional enzyme PaaY from Acinetobacter baumannii. Structure (2023)
Jiao M, He W, Ouyang Z, Qin Q, Guo Y, Zhang J, Bai Y, Guo X, Yu Q, She J, Hwang PM, Zheng F, Wen Y
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 102 nm3

SASDP79 – Chromatin assembly factor 1 - long alpha-helix domain

UniProt ID: Q13111 (342-475) Chromatin assembly factor 1 subunit A
Chromatin assembly factor 1 subunit A experimental SAS data
DAMMIN model
Sample: Chromatin assembly factor 1 subunit A monomer, 18 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.1 mM TCEP, pH: 7
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, Département de Biochimie, Université de Montréal on 2021 Oct 28
Unorthodox PCNA Binding by Chromatin Assembly Factor 1 International Journal of Molecular Sciences 23(19):11099 (2022)
Gopinathan Nair A, Rabas N, Lejon S, Homiski C, Osborne M, Cyr N, Sverzhinsky A, Melendy T, Pascal J, Laue E, Borden K, Omichinski J, Verreault A
RgGuinier 4.1 nm
Dmax 17.2 nm
VolumePorod 54 nm3

SASDP99 – invariant surface glycoprotein ISG65

UniProt ID: C9ZJ67 (18-363) 65 kDa invariant surface glycoprotein, putative
65 kDa invariant surface glycoprotein, putative experimental SAS data
invariant surface glycoprotein ISG65 Rg histogram
Sample: 65 kDa invariant surface glycoprotein, putative monomer, 41 kDa Trypanosoma brucei gambiense … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 25
Cryo-EM structures of Trypanosoma brucei gambiense ISG65 with human complement C3 and C3b and their roles in alternative pathway restriction. Nat Commun 14(1):2403 (2023)
Sülzen H, Began J, Dhillon A, Kereïche S, Pompach P, Votrubova J, Zahedifard F, Šubrtova A, Šafner M, Hubalek M, Thompson M, Zoltner M, Zoll S
RgGuinier 3.3 nm
Dmax 12.5 nm
VolumePorod 53 nm3

SASDPA9 – invariant surface glycoprotein ISG65 bound to complement C3 (C3d)

UniProt ID: C9ZJ67 (18-363) 65 kDa invariant surface glycoprotein, putative

UniProt ID: V9HWA9 (996-1287) Complement C3
65 kDa invariant surface glycoprotein, putativeComplement C3 experimental SAS data
DAMMIF model
Sample: 65 kDa invariant surface glycoprotein, putative monomer, 41 kDa Trypanosoma brucei gambiense … protein
Complement C3 monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 25
Cryo-EM structures of Trypanosoma brucei gambiense ISG65 with human complement C3 and C3b and their roles in alternative pathway restriction. Nat Commun 14(1):2403 (2023)
Sülzen H, Began J, Dhillon A, Kereïche S, Pompach P, Votrubova J, Zahedifard F, Šubrtova A, Šafner M, Hubalek M, Thompson M, Zoltner M, Zoll S
RgGuinier 3.5 nm
Dmax 12.8 nm
VolumePorod 104 nm3

SASDPB9 – SAXS analysis of pilus adhesin PitA from pilus islet-2 of Streptococcus oralis

UniProt ID: D4FSQ3 (36-828) von Willebrand factor type A domain protein
von Willebrand factor type A domain protein experimental SAS data
MODELLER model
Sample: Von Willebrand factor type A domain protein monomer, 89 kDa Streptococcus oralis ATCC … protein
Buffer: 20 mM HEPES,150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 May 12
New structural insights into the PI-2 pilus from Streptococcus oralis, an early dental plaque colonizer. FEBS J (2022)
Yadav RK, Krishnan V
RgGuinier 6.4 nm
Dmax 29.2 nm
VolumePorod 217 nm3

SASDQG4 – Rabies virus Nishigahara strain Phosphoprotein Isoform 3 (P3)

UniProt ID: Q9IPJ8-3 (1-244) Isoform P3 of Phosphoprotein
Isoform P3 of Phosphoprotein experimental SAS data
Rabies virus Nishigahara strain Phosphoprotein Isoform 3 (P3) Rg histogram
Sample: Isoform P3 of Phosphoprotein dimer, 55 kDa Rabies virus (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 26
Structural insights into the multifunctionality of rabies virus P3 protein. Proc Natl Acad Sci U S A 120(14):e2217066120 (2023)
Sethi A, Rawlinson SM, Dubey A, Ang CS, Choi YH, Yan F, Okada K, Rozario AM, Brice AM, Ito N, Williamson NA, Hatters DM, Bell TDM, Arthanari H, Moseley GW, Gooley PR
RgGuinier 3.7 nm
Dmax 16.5 nm
VolumePorod 108 nm3

SASDQM4 – CRISPR associated Lon protease (CalpL)

UniProt ID: B2V8L9 (2-496) SAVED domain-containing protein
SAVED domain-containing protein experimental SAS data
DAMMIF model
Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 14
Antiviral signalling by a cyclic nucleotide activated CRISPR protease. Nature 614(7946):168-174 (2023)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter MF, Boenigk W, Seifert R, Bode BE, Schmid-Burgk JL, Svergun D, Geyer M, White MF, Hagelueken G
RgGuinier 3.1 nm
Dmax 10.3 nm
VolumePorod 80 nm3

SASDQN4 – Complex of CRISPR associated Lon protease (CalpL) with a 10 kDa C-terminal fragment of CalpT a putative MazF-like toxin (CalpT10)

UniProt ID: B2V8L9 (2-496) SAVED domain-containing protein

UniProt ID: B2V8L8 (196-271) Uncharacterized protein (putative MazF-like toxin)
SAVED domain-containing proteinUncharacterized protein (putative MazF-like toxin) experimental SAS data
CUSTOM IN-HOUSE model
Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein
Uncharacterized protein (putative MazF-like toxin) monomer, 9 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 14
Antiviral signalling by a cyclic nucleotide activated CRISPR protease. Nature 614(7946):168-174 (2023)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter MF, Boenigk W, Seifert R, Bode BE, Schmid-Burgk JL, Svergun D, Geyer M, White MF, Hagelueken G
RgGuinier 3.4 nm
Dmax 11.7 nm
VolumePorod 93 nm3

SASDQP4 – CRISPR associated Lon protease (CalpL) in the presence of cyclic oligoadenylate cA4

UniProt ID: B2V8L9 (2-496) SAVED domain-containing protein
SAVED domain-containing protein experimental SAS data
DAMMIF model
Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Apr 8
Antiviral signalling by a cyclic nucleotide activated CRISPR protease. Nature 614(7946):168-174 (2023)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter MF, Boenigk W, Seifert R, Bode BE, Schmid-Burgk JL, Svergun D, Geyer M, White MF, Hagelueken G
RgGuinier 3.2 nm
Dmax 10.3 nm
VolumePorod 82 nm3