SASBDB entries for UniProt ID:

SASDQK9 – An assembly between C-terminal truncated Netrin UNC-6, the extracellular domains of Netrin receptor UNC-5 and UNC-40 with porcine intestinal mucosa heparin (UNC-6ΔC-heparin-UNC-5 ECD-UNC-40 ECD complex)

UniProt ID: P34710 (22-461) Netrin unc-6

UniProt ID: Q26261-1 (1-331) Isoform a of Netrin receptor unc-5

UniProt ID: None (None-None) Heparin, porcine intestinal mucosa

UniProt ID: G5EF96 (23-1082) Netrin receptor unc-40
Netrin unc-6Isoform a of Netrin receptor unc-5Heparin, porcine intestinal mucosaNetrin receptor unc-40 experimental SAS data
Netrin unc-6 Isoform a of Netrin receptor unc-5 Heparin, porcine intestinal mucosa Netrin receptor unc-40 Kratky plot
Sample: Netrin unc-6 monomer, 52 kDa Caenorhabditis elegans protein
Isoform a of Netrin receptor unc-5 monomer, 38 kDa Caenorhabditis elegans protein
Heparin, porcine intestinal mucosa monomer, 15 kDa Sus scrofa domesticus
Netrin receptor unc-40 monomer, 118 kDa Caenorhabditis elegans protein
Buffer: 10 mM HEPES pH 7.2, 150 mM NaCl, 100 mM MgSO4, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Dec 11
Structural insights into the formation of repulsive netrin guidance complexes. Sci Adv 10(7):eadj8083 (2024)
Priest JM, Nichols EL, Smock RG, Hopkins JB, Mendoza JL, Meijers R, Shen K, Özkan E
RgGuinier 10.6 nm
Dmax 36.5 nm
VolumePorod 3410 nm3

SASDQP9 – Hendra virus W protein

UniProt ID: P0C1C6 (2-448) Protein W
Protein W experimental SAS data
Protein W Kratky plot
Sample: Protein W monomer, 53 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Henipavirus W proteins
Frank Gondelaud
RgGuinier 6.8 nm
Dmax 26.0 nm
VolumePorod 250 nm3

SASDQQ9 – Hendra virus P/V/W N-terminal domain of protein W

UniProt ID: P0C1C6 (2-404) Protein W
Protein W experimental SAS data
Protein W Kratky plot
Sample: Protein W monomer, 47 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Henipavirus W proteins
Frank Gondelaud
RgGuinier 6.4 nm
Dmax 32.0 nm

SASDQR9 – Hendra W protein C123-only mutant

UniProt ID: P0C1C6 (2-447) Protein W mutant (C316S, C334S, C419S)
Protein W mutant (C316S, C334S, C419S) experimental SAS data
Protein W mutant (C316S, C334S, C419S) Kratky plot
Sample: Protein W mutant (C316S, C334S, C419S) dimer, 105 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Henipavirus W proteins
Frank Gondelaud
RgGuinier 8.1 nm
Dmax 37.0 nm

SASDQT9 – Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] in complex with fourth BRC repeat (BRC4)

UniProt ID: Q06609 (1-339) DNA repair protein RAD51 homolog 1 (F86E A89E)

UniProt ID: P51587 (1517-1551) Breast cancer type 2 susceptibility protein
DNA repair protein RAD51 homolog 1 (F86E A89E)Breast cancer type 2 susceptibility protein experimental SAS data
Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] in complex with fourth BRC repeat (BRC4) Rg histogram
Sample: DNA repair protein RAD51 homolog 1 (F86E A89E) monomer, 37 kDa Homo sapiens protein
Breast cancer type 2 susceptibility protein monomer, 4 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 50 mM Na2SO4, 2% v/v sucrose, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Dec 7
Isolation and characterization of monomeric human RAD51: a novel tool for investigating homologous recombination in cancer Angewandte Chemie International Edition (2023)
Rinaldi F, Schipani F, Balboni B, Catalano F, Marotta R, Myers S, Previtali V, Veronesi M, Scietti L, Cecatiello V, Pasqualato S, Ortega J, Girotto S, Cavalli A
RgGuinier 2.9 nm
Dmax 15.6 nm
VolumePorod 70 nm3

SASDQU9 – His-Tagged Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] in complex with His-tagged fourth BRC repeat (BRC4)

UniProt ID: Q06609 (1-339) DNA repair protein RAD51 homolog 1 (F86E, A89E, His-Tagged)

UniProt ID: P51587 (1517-1551) Breast cancer type 2 susceptibility protein
DNA repair protein RAD51 homolog 1 (F86E, A89E, His-Tagged)Breast cancer type 2 susceptibility protein experimental SAS data
His-Tagged Monomeric DNA repair protein RAD51 homolog 1 double mutant [F86E, A89E] in complex with His-tagged fourth BRC repeat (BRC4) Rg histogram
Sample: DNA repair protein RAD51 homolog 1 (F86E, A89E, His-Tagged) monomer, 40 kDa Homo sapiens protein
Breast cancer type 2 susceptibility protein monomer, 6 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 200 mM Na2SO4, 5% glycerol, 0.1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Apr 7
Isolation and characterization of monomeric human RAD51: a novel tool for investigating homologous recombination in cancer Angewandte Chemie International Edition (2023)
Rinaldi F, Schipani F, Balboni B, Catalano F, Marotta R, Myers S, Previtali V, Veronesi M, Scietti L, Cecatiello V, Pasqualato S, Ortega J, Girotto S, Cavalli A
RgGuinier 3.0 nm
Dmax 16.5 nm
VolumePorod 78 nm3

SASDQV9 – Nanobody1 in complex with the extracellular domain of human BCMA

UniProt ID: Q02223 (1-54) Tumor necrosis factor receptor superfamily member 17

UniProt ID: None (None-None) Nanobody1
Tumor necrosis factor receptor superfamily member 17Nanobody1 experimental SAS data
Tumor necrosis factor receptor superfamily member 17 Nanobody1 Kratky plot
Sample: Tumor necrosis factor receptor superfamily member 17 monomer, 6 kDa Homo sapiens protein
Nanobody1 monomer, 14 kDa protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Aug 28
Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity. Signal Transduct Target Ther 8(1):445 (2023)
Sun Y, Yang XN, Yang SS, Lyu YZ, Zhang B, Liu KW, Li N, Cui JC, Huang GX, Liu CL, Xu J, Mi JQ, Chen Z, Fan XH, Chen SJ, Chen S
RgGuinier 3.0 nm
Dmax 9.8 nm
VolumePorod 52 nm3

SASDQW9 – Nanobody2 in complex with the extracellular domain of human BCMA

UniProt ID: Q02223 (1-54) Tumor necrosis factor receptor superfamily member 17

UniProt ID: None (None-None) Nanobody2
Tumor necrosis factor receptor superfamily member 17Nanobody2 experimental SAS data
Tumor necrosis factor receptor superfamily member 17 Nanobody2 Kratky plot
Sample: Tumor necrosis factor receptor superfamily member 17 monomer, 6 kDa Homo sapiens protein
Nanobody2 monomer, 14 kDa protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Aug 28
Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity. Signal Transduct Target Ther 8(1):445 (2023)
Sun Y, Yang XN, Yang SS, Lyu YZ, Zhang B, Liu KW, Li N, Cui JC, Huang GX, Liu CL, Xu J, Mi JQ, Chen Z, Fan XH, Chen SJ, Chen S
RgGuinier 2.0 nm
Dmax 9.0 nm
VolumePorod 24 nm3

SASDQX9 – Tandem nanobody in complex with the extracellular domain of human BCMA

UniProt ID: Q02223 (1-54) Tumor necrosis factor receptor superfamily member 17

UniProt ID: None (None-None) Tandem nanobody
Tumor necrosis factor receptor superfamily member 17Tandem nanobody experimental SAS data
Tumor necrosis factor receptor superfamily member 17 Tandem nanobody Kratky plot
Sample: Tumor necrosis factor receptor superfamily member 17 monomer, 6 kDa Homo sapiens protein
Tandem nanobody monomer, 27 kDa protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Aug 28
Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity. Signal Transduct Target Ther 8(1):445 (2023)
Sun Y, Yang XN, Yang SS, Lyu YZ, Zhang B, Liu KW, Li N, Cui JC, Huang GX, Liu CL, Xu J, Mi JQ, Chen Z, Fan XH, Chen SJ, Chen S
RgGuinier 5.1 nm
Dmax 23.3 nm
VolumePorod 247 nm3

SASDQY9 – Beclin-1 amino acids 1-150 with cysteine motif residues mutated to serine, along with a C-terminal tyrosine

UniProt ID: Q14457 (1-150) Beclin-1 (C18S, C21S, A103V, C137S, C140S)
Beclin-1 (C18S, C21S, A103V, C137S, C140S) experimental SAS data
Beclin-1 amino acids 1-150 with cysteine motif residues mutated to serine, along with a C-terminal tyrosine Rg histogram
Sample: Beclin-1 (C18S, C21S, A103V, C137S, C140S) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM Tris, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 12
Invariant BECN1 CXXC motifs bind Zn(2+) and regulate structure and function of the BECN1 intrinsically disordered region. Autophagy :1-17 (2023)
Mukhopadhyay S, Subedi S, Hopkins JB, Ugrinov A, Chakravarthy S, Colbert CL, Sinha SC
RgGuinier 4.1 nm
Dmax 19.3 nm
VolumePorod 57 nm3