SASBDB entries for UniProt ID:

SASDSW2 – spikemoss tandem zinc finger (TZF) domain, pH 8 at 4.1 mg/mL

UniProt ID: D8SB57 (10-76) C3H1-type domain-containing protein
C3H1-type domain-containing protein experimental SAS data
C3H1-type domain-containing protein Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.7 nm
Dmax 7.1 nm
VolumePorod 14 nm3

SASDSX2 – spikemoss tandem zinc finger (TZF) domain, pH 8 at 2.75 mg/mL

UniProt ID: D8SB57 (10-76) C3H1-type domain-containing protein
C3H1-type domain-containing protein experimental SAS data
C3H1-type domain-containing protein Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.7 nm
Dmax 7.1 nm
VolumePorod 14 nm3

SASDS33 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 5 mg/mL

UniProt ID: D8SB57 (10-76) C3H1-type domain-containing protein

UniProt ID: None (10-76) single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence
C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data
C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.6 nm
Dmax 5.6 nm
VolumePorod 13 nm3

SASDS43 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 3 mg/mL

UniProt ID: None (10-76) single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence

UniProt ID: D8SB57 (10-76) C3H1-type domain-containing protein
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequenceC3H1-type domain-containing protein experimental SAS data
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence C3H1-type domain-containing protein Kratky plot
Sample: Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.5 nm
Dmax 5.7 nm
VolumePorod 13 nm3

SASDS53 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 2 mg/mL

UniProt ID: D8SB57 (10-76) C3H1-type domain-containing protein

UniProt ID: None (10-76) single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence
C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data
C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.5 nm
Dmax 5.6 nm
VolumePorod 13 nm3

SASDS63 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 6 mg/mL

UniProt ID: D8SB57 (10-76) C3H1-type domain-containing protein

UniProt ID: None (10-76) single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence
C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data
C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.5 nm
Dmax 5.1 nm
VolumePorod 13 nm3

SASDS73 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 4.5 mg/mL

UniProt ID: D8SB57 (10-76) C3H1-type domain-containing protein

UniProt ID: None (10-76) single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence
C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data
C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.5 nm
Dmax 4.9 nm
VolumePorod 13 nm3

SASDS83 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 3 mg/mL

UniProt ID: D8SB57 (10-76) C3H1-type domain-containing protein

UniProt ID: None (10-76) single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence
C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data
C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.5 nm
Dmax 5.3 nm
VolumePorod 13 nm3

SASDSZ4 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) unliganded form

UniProt ID: P04585 (588-1147) Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)

UniProt ID: P04585 (588-1027) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT)
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) experimental SAS data
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) Kratky plot
Sample: Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 3.3 nm
Dmax 9.9 nm
VolumePorod 153 nm3

SASDS25 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Nevirapine

UniProt ID: P04585 (588-1147) Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)

UniProt ID: P04585 (588-1027) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT)
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) experimental SAS data
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) Kratky plot
Sample: Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 3.5 nm
Dmax 10.9 nm
VolumePorod 161 nm3