SASBDB entries for UniProt ID:

SASDS35 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) unliganded form

UniProt ID: P04585 (588-1147) Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66)
Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) experimental SAS data
Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) Kratky plot
Sample: Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 3.1 nm
Dmax 10.7 nm
VolumePorod 98 nm3

SASDS45 – HIV-1 Reverse Transcriptase heterodimer (p66/p51) bound with Efavirenz

UniProt ID: P04585 (588-1147) Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)

UniProt ID: P04585 (588-1027) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT)
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66)Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) experimental SAS data
Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) Kratky plot
Sample: Gag-Pol polyprotein (588-1147: Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Gag-Pol polyprotein (588-1027: Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 3.7 nm
Dmax 11.3 nm
VolumePorod 178 nm3

SASDS55 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) bound with Nevirapine

UniProt ID: P04585 (588-1147) Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66)
Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) experimental SAS data
Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) Kratky plot
Sample: Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 3.5 nm
Dmax 11.3 nm
VolumePorod 134 nm3

SASDS65 – HIV-1 Reverse Transcriptase monomer (p66 subunit with W401A mutation) bound with Efavirenz

UniProt ID: P04585 (588-1147) Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66)
Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) experimental SAS data
Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) Kratky plot
Sample: Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66) monomer, 67 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 4.6 nm
Dmax 15.0 nm
VolumePorod 270 nm3

SASDS75 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) unliganded form

UniProt ID: P04585 (588-1027) Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT)
Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) experimental SAS data
Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) Kratky plot
Sample: Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 2.8 nm
Dmax 9.8 nm
VolumePorod 84 nm3

SASDS85 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) bound with Nevirapine

UniProt ID: P04585 (588-1027) Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT)
Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) experimental SAS data
Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) Kratky plot
Sample: Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 3.1 nm
Dmax 11.1 nm
VolumePorod 103 nm3

SASDS95 – HIV-1 Reverse Transcriptase monomer (p51 subunit with W401A mutation) bound with Efavirenz

UniProt ID: P04585 (588-1027) Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT)
Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) experimental SAS data
Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) Kratky plot
Sample: Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT) monomer, 53 kDa Human immunodeficiency virus … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2017 Jun 19
Biophysical Characterization of p51 and p66 Monomers of HIV-1 Reverse Transcriptase with Their Inhibitors. Protein J (2023)
Seetaha S, Kamonsutthipaijit N, Yagi-Utsumi M, Seako Y, Yamaguchi T, Hannongbua S, Kato K, Choowongkomon K
RgGuinier 4.2 nm
Dmax 16.7 nm
VolumePorod 226 nm3

SASDSA5 – dark-state short-linker LOV-activated diguanylate cyclase

UniProt ID: A0A2I7N2Y9 (13-304) Diguanylate cyclase
Diguanylate cyclase experimental SAS data
Diguanylate cyclase Kratky plot
Sample: Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein
Buffer: 10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 3.4 nm
Dmax 13.6 nm
VolumePorod 102 nm3

SASDSB5 – light-state short-linker LOV-activated diguanylate cyclase

UniProt ID: A0A2I7N2Y9 (13-304) Diguanylate cyclase
Diguanylate cyclase experimental SAS data
Diguanylate cyclase Kratky plot
Sample: Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein
Buffer: 10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 3.4 nm
Dmax 15.7 nm
VolumePorod 104 nm3

SASDSC5 – dark-state long-linker LOV-activated diguanylate cyclase

UniProt ID: A0A2S5LZS0 (3-314) Sensor domain-containing diguanylate cyclase
Sensor domain-containing diguanylate cyclase experimental SAS data
GASBOR model
Sample: Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer: 10 mM Tris, 50 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 2.9 nm
Dmax 11.1 nm
VolumePorod 115 nm3