SASBDB entries for UniProt ID:

SASDSE7 – Human CDAN1-interacting nuclease 1 (CDIN1): analysis of SEC peak

UniProt ID: Q9Y2V0 (1-281) CDAN1-interacting nuclease 1
CDAN1-interacting nuclease 1 experimental SAS data
ALPHAFOLD model
Sample: CDAN1-interacting nuclease 1 dimer, 67 kDa Homo sapiens protein
Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2021 Sep 2
Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex
Tomas Brom
RgGuinier 3.0 nm
Dmax 8.2 nm
VolumePorod 84 nm3

SASDSF7 – Human Codanin1 (C-terminus): analysis of second monomeric SEC peak

UniProt ID: Q8IWY9 (1005-1227) Codanin-1
Codanin-1 experimental SAS data
ALPHAFOLD model
Sample: Codanin-1 monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Mar 5
Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex
Tomas Brom
RgGuinier 2.7 nm
Dmax 7.5 nm
VolumePorod 54 nm3

SASDSG7 – Human Codanin1 (C-terminus): analysis of first oligomeric SEC peak

UniProt ID: Q8IWY9 (1005-1227) Codanin-1
Codanin-1 experimental SAS data
ALPHAFOLD model
Sample: Codanin-1 monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Mar 5
Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex
Tomas Brom
RgGuinier 4.1 nm
Dmax 10.7 nm
VolumePorod 220 nm3

SASDSM7 – Full-length Heat shock factor 2-binding protein (HSF2BP)

UniProt ID: O75031 (2-334) Heat shock factor 2-binding protein
Heat shock factor 2-binding protein experimental SAS data
Heat shock factor 2-binding protein Kratky plot
Sample: Heat shock factor 2-binding protein tetramer, 150 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl pH 7.5, 250 mM NaCl, and 5 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Oct 2
BRCA2-HSF2BP oligomeric ring disassembly by BRME1 promotes homologous recombination. Sci Adv 9(43):eadi7352 (2023)
Ghouil R, Miron S, Sato K, Ristic D, van Rossum-Fikkert SE, Legrand P, Ouldali M, Winter JM, Ropars V, David G, Arteni AA, Wyman C, Knipscheer P, Kanaar R, Zelensky AN, Zinn-Justin S
RgGuinier 8.5 nm
Dmax 25.0 nm
VolumePorod 364 nm3

SASDSN7 – Full length human mature gelsolin in closed state

UniProt ID: P06396 (28-782) Gelsolin
Gelsolin experimental SAS data
GROMACS model
Sample: Gelsolin monomer, 85 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 24
Accurate and Efficient SAXS/SANS Implementation Including Solvation Layer Effects Suitable for Molecular Simulations. J Chem Theory Comput (2023)
Ballabio F, Paissoni C, Bollati M, de Rosa M, Capelli R, Camilloni C
RgGuinier 3.2 nm
Dmax 11.1 nm
VolumePorod 128 nm3

SASDSY7 – Karrikin insensitive 2, KAI2 protein (monomer peak)

UniProt ID: Q9SZU7 (None-None) Probable esterase KAI2 (KARRIKIN INSENSITIVE 2)
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 58 nm3

SASDSZ7 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (monomer peak)

UniProt ID: Q9SZU7 (None-None) Probable esterase KAI2 (KARRIKIN INSENSITIVE 2)
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.9 nm
Dmax 13.9 nm
VolumePorod 60 nm3

SASDS28 – Karrikin insensitive 2, KAI2 protein (aggregate peak)

UniProt ID: Q9SZU7 (None-None) Probable esterase KAI2 (KARRIKIN INSENSITIVE 2)
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 4.1 nm
Dmax 15.8 nm
VolumePorod 283 nm3

SASDS38 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (aggregate peak)

UniProt ID: Q9SZU7 (None-None) Probable esterase KAI2 (KARRIKIN INSENSITIVE 2)
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 4.9 nm
Dmax 20.0 nm
VolumePorod 345 nm3

SASDS48 – Karrikin insensitive 2, KAI2 protein mutant S95A (monomer peak)

UniProt ID: Q9SZU7 (1-270) Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A)
Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) experimental SAS data
Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) Kratky plot
Sample: Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
RgGuinier 1.9 nm
Dmax 6.3 nm
VolumePorod 55 nm3