SASBDB entries for UniProt ID:

SASDSE7 – Human CDAN1-interacting nuclease 1 (CDIN1): analysis of SEC peak

UniProt ID: Q9Y2V0 (1-281) CDAN1-interacting nuclease 1
CDAN1-interacting nuclease 1 experimental SAS data
ALPHAFOLD model
Sample: CDAN1-interacting nuclease 1 dimer, 67 kDa Homo sapiens protein
Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2021 Sep 2
Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex
Tomas Brom
RgGuinier 3.0 nm
Dmax 8.2 nm
VolumePorod 84 nm3

SASDSF7 – Human Codanin1 (C-terminus): analysis of second monomeric SEC peak

UniProt ID: Q8IWY9 (1005-1227) Codanin-1
Codanin-1 experimental SAS data
ALPHAFOLD model
Sample: Codanin-1 monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Mar 5
Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex
Tomas Brom
RgGuinier 2.7 nm
Dmax 7.5 nm
VolumePorod 54 nm3

SASDSG7 – Human Codanin1 (C-terminus): analysis of first oligomeric SEC peak

UniProt ID: Q8IWY9 (1005-1227) Codanin-1
Codanin-1 experimental SAS data
ALPHAFOLD model
Sample: Codanin-1 monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Mar 5
Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex
Tomas Brom
RgGuinier 4.1 nm
Dmax 10.7 nm
VolumePorod 220 nm3

SASDSM7 – Full-length Heat shock factor 2-binding protein (HSF2BP)

UniProt ID: O75031 (2-334) Heat shock factor 2-binding protein
Heat shock factor 2-binding protein experimental SAS data
Heat shock factor 2-binding protein Kratky plot
Sample: Heat shock factor 2-binding protein tetramer, 150 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl pH 7.5, 250 mM NaCl, and 5 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Oct 2
BRCA2-HSF2BP oligomeric ring disassembly by BRME1 promotes homologous recombination. Sci Adv 9(43):eadi7352 (2023)
Ghouil R, Miron S, Sato K, Ristic D, van Rossum-Fikkert SE, Legrand P, Ouldali M, Winter JM, Ropars V, David G, Arteni AA, Wyman C, Knipscheer P, Kanaar R, Zelensky AN, Zinn-Justin S
RgGuinier 8.5 nm
Dmax 25.0 nm
VolumePorod 364 nm3

SASDSN7 – Full length human mature gelsolin in closed state

UniProt ID: P06396 (28-782) Gelsolin
Gelsolin experimental SAS data
GROMACS model
Sample: Gelsolin monomer, 85 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 24
Accurate and Efficient SAXS/SANS Implementation Including Solvation Layer Effects Suitable for Molecular Simulations. J Chem Theory Comput (2023)
Ballabio F, Paissoni C, Bollati M, de Rosa M, Capelli R, Camilloni C
RgGuinier 3.2 nm
Dmax 11.1 nm
VolumePorod 128 nm3

SASDSR7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form without any substrates

UniProt ID: P0C061 (1-1098) Gramicidin S synthase 1 (W239S)
Gramicidin S synthase 1 (W239S) experimental SAS data
BILBOMD model
Sample: Gramicidin S synthase 1 (W239S) monomer, 127 kDa Aneurinibacillus migulanus protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 187 nm3

SASDSS7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form in the presence of 4 molar excess of L-Tyrosine

UniProt ID: P0C062 (1-1098) Gramicidin S synthase 1 (W239S)
Gramicidin S synthase 1 (W239S) experimental SAS data
Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.3 nm
Dmax 15.4 nm
VolumePorod 186 nm3

SASDST7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form in the presence of 4 molar excess of L-Tyrosine and ATP analog (AMPPCP)

UniProt ID: P0C062 (1-1098) Gramicidin S synthase 1 (W239S)
Gramicidin S synthase 1 (W239S) experimental SAS data
Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.2 nm
Dmax 16.0 nm
VolumePorod 186 nm3

SASDSU7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the holo form in the presence of 4 molar excess of L-Tyrosine

UniProt ID: P0C062 (1-1098) Gramicidin S synthase 1 (W239S)
Gramicidin S synthase 1 (W239S) experimental SAS data
Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.1 nm
Dmax 17.5 nm
VolumePorod 181 nm3

SASDSV7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the holo form in the presence of 4 molar excess of L-Tyrosine and ATP analog (AMPPCP)

UniProt ID: P0C062 (1-1098) Gramicidin S synthase 1 (W239S)
Gramicidin S synthase 1 (W239S) experimental SAS data
Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.0 nm
Dmax 14.3 nm
VolumePorod 175 nm3