SASBDB entries for UniProt ID:

SASDSA7 – polymerase alpha-primase polΔcat-prim bound to DNA in the presence of an RNA-primed template

UniProt ID: Q14181 (155-598) DNA polymerase alpha subunit B

UniProt ID: P09884 (1265-1462) DNA polymerase alpha catalytic subunit

UniProt ID: P49643 (1-509) DNA primase large subunit

UniProt ID: P49642 (1-420) DNA primase small subunit

UniProt ID: None (None-None) 29 mer DNA template

UniProt ID: None (None-None) 8mer RNA primer
DNA polymerase alpha subunit BDNA polymerase alpha catalytic subunitDNA primase large subunitDNA primase small subunit29 mer DNA template8mer RNA primer experimental SAS data
DNA polymerase alpha subunit B DNA polymerase alpha catalytic subunit DNA primase large subunit DNA primase small subunit 29 mer DNA template 8mer RNA primer Kratky plot
Sample: DNA polymerase alpha subunit B monomer, 49 kDa Homo sapiens protein
DNA polymerase alpha catalytic subunit monomer, 23 kDa Homo sapiens protein
DNA primase large subunit monomer, 59 kDa Homo sapiens protein
DNA primase small subunit monomer, 50 kDa Homo sapiens protein
29 mer DNA template monomer, 9 kDa DNA
8mer RNA primer monomer, 3 kDa RNA
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 28
Flexibility and distributive synthesis regulate RNA priming and handoff in human DNA polymerase α-primase Journal of Molecular Biology :168330 (2023)
Cordoba J, Mullins E, Salay L, Eichman B, Chazin W
RgGuinier 4.9 nm
Dmax 15.3 nm
VolumePorod 344 nm3

SASDSB7 – polymerase alpha-primase polΔcat-primΔ2C in the absence of substrate

UniProt ID: Q14181 (155-598) DNA polymerase alpha subunit B

UniProt ID: P09884 (1265-1462) DNA polymerase alpha catalytic subunit

UniProt ID: P49642 (1-420) DNA primase small subunit

UniProt ID: P49643 (1-265) DNA primase large subunit
DNA polymerase alpha subunit BDNA polymerase alpha catalytic subunitDNA primase small subunitDNA primase large subunit experimental SAS data
DNA polymerase alpha subunit B DNA polymerase alpha catalytic subunit DNA primase small subunit DNA primase large subunit Kratky plot
Sample: DNA polymerase alpha subunit B monomer, 49 kDa Homo sapiens protein
DNA polymerase alpha catalytic subunit monomer, 23 kDa Homo sapiens protein
DNA primase small subunit monomer, 50 kDa Homo sapiens protein
DNA primase large subunit monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Apr 15
Flexibility and distributive synthesis regulate RNA priming and handoff in human DNA polymerase α-primase Journal of Molecular Biology :168330 (2023)
Cordoba J, Mullins E, Salay L, Eichman B, Chazin W
RgGuinier 4.5 nm
Dmax 14.5 nm
VolumePorod 221 nm3

SASDSE7 – Human CDAN1-interacting nuclease 1 (CDIN1): analysis of SEC peak

UniProt ID: Q9Y2V0 (1-281) CDAN1-interacting nuclease 1
CDAN1-interacting nuclease 1 experimental SAS data
ALPHAFOLD model
Sample: CDAN1-interacting nuclease 1 dimer, 67 kDa Homo sapiens protein
Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2021 Sep 2
Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex
Tomas Brom
RgGuinier 3.0 nm
Dmax 8.2 nm
VolumePorod 84 nm3

SASDSF7 – Human Codanin1 (C-terminus): analysis of second monomeric SEC peak

UniProt ID: Q8IWY9 (1005-1227) Codanin-1
Codanin-1 experimental SAS data
ALPHAFOLD model
Sample: Codanin-1 monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Mar 5
Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex
Tomas Brom
RgGuinier 2.7 nm
Dmax 7.5 nm
VolumePorod 54 nm3

SASDSG7 – Human Codanin1 (C-terminus): analysis of first oligomeric SEC peak

UniProt ID: Q8IWY9 (1005-1227) Codanin-1
Codanin-1 experimental SAS data
ALPHAFOLD model
Sample: Codanin-1 monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM Tris HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Mar 5
Structural insights into inherited anemia CDA-I: disease-associated mutations disrupt Codanin1-CDIN1 complex
Tomas Brom
RgGuinier 4.1 nm
Dmax 10.7 nm
VolumePorod 220 nm3

SASDSM7 – Full-length Heat shock factor 2-binding protein (HSF2BP)

UniProt ID: O75031 (2-334) Heat shock factor 2-binding protein
Heat shock factor 2-binding protein experimental SAS data
Heat shock factor 2-binding protein Kratky plot
Sample: Heat shock factor 2-binding protein tetramer, 150 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl pH 7.5, 250 mM NaCl, and 5 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Oct 2
BRCA2-HSF2BP oligomeric ring disassembly by BRME1 promotes homologous recombination. Sci Adv 9(43):eadi7352 (2023)
Ghouil R, Miron S, Sato K, Ristic D, van Rossum-Fikkert SE, Legrand P, Ouldali M, Winter JM, Ropars V, David G, Arteni AA, Wyman C, Knipscheer P, Kanaar R, Zelensky AN, Zinn-Justin S
RgGuinier 8.5 nm
Dmax 25.0 nm
VolumePorod 364 nm3

SASDSN7 – Full length human mature gelsolin in closed state

UniProt ID: P06396 (28-782) Gelsolin
Gelsolin experimental SAS data
GROMACS model
Sample: Gelsolin monomer, 85 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 24
Accurate and Efficient SAXS/SANS Implementation Including Solvation Layer Effects Suitable for Molecular Simulations. J Chem Theory Comput (2023)
Ballabio F, Paissoni C, Bollati M, de Rosa M, Capelli R, Camilloni C
RgGuinier 3.2 nm
Dmax 11.1 nm
VolumePorod 128 nm3

SASDSR7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form without any substrates

UniProt ID: P0C061 (1-1098) Gramicidin S synthase 1 (W239S)
Gramicidin S synthase 1 (W239S) experimental SAS data
BILBOMD model
Sample: Gramicidin S synthase 1 (W239S) monomer, 127 kDa Aneurinibacillus migulanus protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 187 nm3

SASDSS7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form in the presence of 4 molar excess of L-Tyrosine

UniProt ID: P0C062 (1-1098) Gramicidin S synthase 1 (W239S)
Gramicidin S synthase 1 (W239S) experimental SAS data
Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.3 nm
Dmax 15.4 nm
VolumePorod 186 nm3

SASDST7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form in the presence of 4 molar excess of L-Tyrosine and ATP analog (AMPPCP)

UniProt ID: P0C062 (1-1098) Gramicidin S synthase 1 (W239S)
Gramicidin S synthase 1 (W239S) experimental SAS data
Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.2 nm
Dmax 16.0 nm
VolumePorod 186 nm3