Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDSU7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the holo form in the presence of 4 molar excess of L-Tyrosine

UniProt ID: P0C062 (1-1098) Gramicidin S synthase 1 (W239S)

Gramicidin S synthase 1 (W239S) experimental SAS data

Sample:	Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer:	20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14

Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy

R_g^Guinier	4.1	nm
D_max	17.5	nm
Volume^Porod	181	nm³

SASDSV7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the holo form in the presence of 4 molar excess of L-Tyrosine and ATP analog (AMPPCP)

UniProt ID: P0C062 (1-1098) Gramicidin S synthase 1 (W239S)

Sample:	Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer:	20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14

Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy

R_g^Guinier	4.0	nm
D_max	14.3	nm
Volume^Porod	175	nm³

SASDSY7 – Karrikin insensitive 2, KAI2 protein (monomer peak)

UniProt ID: Q9SZU7 (None-None) Probable esterase KAI2 (KARRIKIN INSENSITIVE 2)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	2.3	nm
D_max	9.5	nm
Volume^Porod	58	nm³

SASDSZ7 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (monomer peak)

UniProt ID: Q9SZU7 (None-None) Probable esterase KAI2 (KARRIKIN INSENSITIVE 2)

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	2.9	nm
D_max	13.9	nm
Volume^Porod	60	nm³

SASDS28 – Karrikin insensitive 2, KAI2 protein (aggregate peak)

UniProt ID: Q9SZU7 (None-None) Probable esterase KAI2 (KARRIKIN INSENSITIVE 2)

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	4.1	nm
D_max	15.8	nm
Volume^Porod	283	nm³

SASDS38 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (aggregate peak)

UniProt ID: Q9SZU7 (None-None) Probable esterase KAI2 (KARRIKIN INSENSITIVE 2)

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	4.9	nm
D_max	20.0	nm
Volume^Porod	345	nm³

SASDS48 – Karrikin insensitive 2, KAI2 protein mutant S95A (monomer peak)

UniProt ID: Q9SZU7 (1-270) Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A)

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) experimental SAS data

Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) Kratky plot

Sample:	Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants

R_g^Guinier	1.9	nm
D_max	6.3	nm
Volume^Porod	55	nm³

SASDS58 – Karrikin insensitive 2, KAI2 protein mutant S95A with a synthetic strigolactone analogue ligand (monomer peak)

UniProt ID: Q9SZU7 (1-270) Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A)

Sample:	Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	2.1	nm
D_max	7.2	nm
Volume^Porod	61	nm³

SASDS68 – Karrikin insensitive 2, KAI2 protein mutant S95A (aggregate peak)

UniProt ID: Q9SZU7 (1-270) Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A)

Sample:	Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	3.9	nm
D_max	13.2	nm
Volume^Porod	171	nm³

SASDS78 – Karrikin insensitive 2, KAI2 protein mutant S95A with a synthetic strigolactone analogue ligand (aggregate peak)

UniProt ID: Q9SZU7 (1-270) Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A)

Sample:	Probable esterase KAI2 - S95A (KARRIKIN INSENSITIVE 2 - S95A) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	4.5	nm
D_max	19.3	nm
Volume^Porod	285	nm³

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