Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDSA8 – Karrikin insensitive 2, KAI2 protein mutant H246Y (monomer peak)

UniProt ID: Q9SZU7 (1-270) Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y)

Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) experimental SAS data

Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) Kratky plot

Sample:	Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	3.0	nm
D_max	16.1	nm
Volume^Porod	70	nm³

SASDSB8 – Karrikin insensitive 2, KAI2 protein mutant H246Y with a synthetic strigolactone analogue ligand (monomer peak)

UniProt ID: Q9SZU7 (1-270) Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y)

Sample:	Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	2.9	nm
D_max	13.2	nm
Volume^Porod	68	nm³

SASDSC8 – Karrikin insensitive 2, KAI2 protein mutant H246Y (aggregate peak)

UniProt ID: Q9SZU7 (1-270) Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y)

Sample:	Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	5.6	nm
D_max	23.0	nm
Volume^Porod	494	nm³

SASDSD8 – Karrikin insensitive 2, KAI2 protein mutant H246Y with a synthetic strigolactone analogue ligand (aggregate peak)

UniProt ID: Q9SZU7 (1-270) Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y)

Sample:	Probable esterase KAI2 - H246Y (KARRIKIN INSENSITIVE 2 - H246Y) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	5.1	nm
D_max	19.3	nm
Volume^Porod	403	nm³

SASDSE8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 0.27 mg/mL

UniProt ID: Q8IZF6 (26-240) Adhesion G-protein coupled receptor G4

Adhesion G-protein coupled receptor G4 experimental SAS data

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

The dimerized pentraxin-like domain of the adhesion G protein-coupled receptor 112 (ADGRG4) suggests function in sensing mechanical forces. J Biol Chem :105356 (2023)
Kieslich B, Weiße RH, Brendler J, Ricken A, Schöneberg T, Sträter N

R_g^Guinier	2.7	nm
D_max	7.1	nm
Volume^Porod	67	nm³

SASDSF8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 0.55 mg/mL

UniProt ID: Q8IZF6 (26-240) Adhesion G-protein coupled receptor G4

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	2.8	nm
D_max	8.0	nm
Volume^Porod	65	nm³

SASDSG8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 1.07 mg/mL

UniProt ID: Q8IZF6 (26-240) Adhesion G-protein coupled receptor G4

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	2.9	nm
D_max	9.0	nm
Volume^Porod	70	nm³

SASDSH8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 1.88 mg/mL

UniProt ID: Q8IZF6 (26-240) Adhesion G-protein coupled receptor G4

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	3.0	nm
D_max	9.7	nm
Volume^Porod	74	nm³

SASDSJ8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 4.37 mg/mL

UniProt ID: Q8IZF6 (26-240) Adhesion G-protein coupled receptor G4

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	3.0	nm
D_max	9.9	nm
Volume^Porod	76	nm³

SASDSK8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 6.38 mg/mL

UniProt ID: Q8IZF6 (26-240) Adhesion G-protein coupled receptor G4

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	3.0	nm
D_max	9.9	nm
Volume^Porod	77	nm³

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