SASBDB entries for UniProt ID:

SASDTW3 – Tissue Transglutaminase + 250 µM CaCl2

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.3 nm
Dmax 21.0 nm
VolumePorod 160 nm3

SASDTX3 – Tissue Transglutaminase + 500 µM CaCl2

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.3 nm
Dmax 20.0 nm
VolumePorod 158 nm3

SASDTY3 – Tissue Transglutaminase + 1 mM CaCl2

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.5 nm
Dmax 24.0 nm
VolumePorod 180 nm3

SASDTZ3 – Tissue Transglutaminase + 2 mM CaCl2

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.3 nm
Dmax 25.5 nm
VolumePorod 234 nm3

SASDT24 – Tissue Transglutaminase + 50 µM inhibitor LM11

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.3 nm
Dmax 20.0 nm
VolumePorod 158 nm3

SASDT34 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 250 µM CaCl2

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.2 nm
Dmax 19.0 nm
VolumePorod 155 nm3

SASDT44 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 500 µM CaCl2

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.9 nm
Dmax 25.0 nm
VolumePorod 191 nm3

SASDT54 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 1 mM CaCl2

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.7 nm
Dmax 28.0 nm
VolumePorod 198 nm3

SASDT64 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 2 mM CaCl2

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 5.2 nm
Dmax 25.0 nm
VolumePorod 236 nm3

SASDT74 – Tissue Transglutaminase + CaCl2 + inhibitor LM11: Equilibrium

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM LM11, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 6.0 nm
Dmax 36.0 nm
VolumePorod 431 nm3