SASBDB entries for UniProt ID:

SASDTS2 – Turkey Avisivirus 2A3 (TuAsV 2A3) polyprotein fragment at pH 6.5

UniProt ID: M1L6N7 (944-1066) Genome polyprotein
Genome polyprotein experimental SAS data
ALPHAFOLD model
Sample: Genome polyprotein monomer, 13 kDa Turkey avisivirus protein
Buffer: 20mM Citrate buffer pH 6.5, 150mM NaCl, 2mM TCEP, 1% glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 May 15
Solution structure and functional characterization of an avian picornavirus 2AH/NC protein
Anna Wehlin
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 27 nm3

SASDTT2 – Turkey Avisivirus 2A3 (TuAsV 2A3) polyprotein fragment at pH 7.4

UniProt ID: M1L6N7 (944-1066) Genome polyprotein
Genome polyprotein experimental SAS data
ALPHAFOLD model
Sample: Genome polyprotein monomer, 13 kDa Turkey avisivirus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM TECP, 1% Glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 2
Solution structure and functional characterization of an avian picornavirus 2AH/NC protein
Anna Wehlin
RgGuinier 1.6 nm
Dmax 4.0 nm
VolumePorod 24 nm3

SASDTU2 – NAD-dependent protein deacetylase sirtuin-7 at pH 8.0

UniProt ID: Q9NRC8-1 (1-400) Isoform 1 of NAD-dependent protein deacetylase sirtuin-7
Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 experimental SAS data
CORAL model
Sample: Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 monomer, 45 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 16
Substrates and Cyclic Peptide Inhibitors of the Oligonucleotide-Activated Sirtuin 7. Angew Chem Int Ed Engl :e202314597 (2023)
Bolding JE, Nielsen AL, Jensen I, Hansen TN, Ryberg LA, Jameson ST, Harris P, Peters GHJ, Denu JM, Rogers JM, Olsen CA
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 89 nm3

SASDTW2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme wildtype

UniProt ID: P30153 (1-589) Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

UniProt ID: Q14738-1 (1-602) Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H)

UniProt ID: P67775-1 (1-309) Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoformIsoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H)Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform experimental SAS data
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H) Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Kratky plot
Sample: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform monomer, 65 kDa Homo sapiens protein
Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H) monomer, 70 kDa Homo sapiens protein
Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform monomer, 36 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 50 µM MnCl2, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 20
B56δ long-disordered arms form a dynamic PP2A regulation interface coupled with global allostery and Jordan's syndrome mutations. Proc Natl Acad Sci U S A 121(1):e2310727120 (2024)
Wu CG, Balakrishnan VK, Merrill RA, Parihar PS, Konovolov K, Chen YC, Xu Z, Wei H, Sundaresan R, Cui Q, Wadzinski BE, Swingle MR, Musiyenko A, Chung WK, Honkanen RE, Suzuki A, Huang X, Strack S, Xing Y
RgGuinier 4.0 nm
Dmax 12.0 nm
VolumePorod 266 nm3

SASDTX2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme E198K mutant

UniProt ID: P30153 (1-589) Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

UniProt ID: P67775-1 (1-309) Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

UniProt ID: Q14738-1 (1-602) Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H, E198K)
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoformIsoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoformIsoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H, E198K) experimental SAS data
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H, E198K) Kratky plot
Sample: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform monomer, 65 kDa Homo sapiens protein
Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform monomer, 36 kDa Homo sapiens protein
Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H, E198K) monomer, 70 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 50 µM MnCl2, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 20
B56δ long-disordered arms form a dynamic PP2A regulation interface coupled with global allostery and Jordan's syndrome mutations. Proc Natl Acad Sci U S A 121(1):e2310727120 (2024)
Wu CG, Balakrishnan VK, Merrill RA, Parihar PS, Konovolov K, Chen YC, Xu Z, Wei H, Sundaresan R, Cui Q, Wadzinski BE, Swingle MR, Musiyenko A, Chung WK, Honkanen RE, Suzuki A, Huang X, Strack S, Xing Y
RgGuinier 4.2 nm
Dmax 14.0 nm
VolumePorod 305 nm3

SASDTK3 – Human aryl-hydrocarbon receptor-interacting protein (R9Q point mutant)

UniProt ID: O00170 (2-330) AH receptor-interacting protein (R9Q)
AH receptor-interacting protein (R9Q) experimental SAS data
OTHER model
Sample: AH receptor-interacting protein (R9Q) monomer, 39 kDa Homo sapiens protein
Buffer: 20 mM Tris-Cl pH 7.5, 100 mM NaCl, 1 mM DTT, 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jun 13
Human AIP HSP90 complex
Robert Rambo
RgGuinier 3.0 nm
Dmax 10.9 nm
VolumePorod 64 nm3

SASDTL3 – Tissue Transglutaminase: Equilibrium

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 4.2 nm
Dmax 20.0 nm
VolumePorod 148 nm3

SASDTM3 – Tissue Transglutaminase + 25 µM GTP

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.8 nm
Dmax 17.5 nm
VolumePorod 132 nm3

SASDTN3 – Tissue Transglutaminase + 50 µM GTP

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.7 nm
Dmax 17.5 nm
VolumePorod 124 nm3

SASDTP3 – Tissue Transglutaminase + 100 µM GTP

UniProt ID: P21980 (1-687) Protein-glutamine gamma-glutamyltransferase 2
Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data
Protein-glutamine gamma-glutamyltransferase 2 Kratky plot
Sample: Protein-glutamine gamma-glutamyltransferase 2, 77 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17
Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
Aplin C, Zielinski KA, Pabit S, Ogunribido D, Katt WP, Pollack L, Cerione RA, Milano SK
RgGuinier 3.6 nm
Dmax 18.0 nm
VolumePorod 121 nm3