SASBDB entries for UniProt ID:

SASDUQ7 – Lcl C-terminal domain K526A mutant

UniProt ID: E7BLH6 (387-536) HbP1 (K526A)
HbP1 (K526A) experimental SAS data
HbP1 (K526A) Kratky plot
Sample: HbP1 (K526A) trimer, 56 kDa Legionella pneumophila protein
Buffer: 20 mM Tris–HCl pH 8.0, 200 mM NaCl, 5 mM EDTA, pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans. Nat Commun 15(1):4912 (2024)
Rehman S, Antonovic AK, McIntire IE, Zheng H, Cleaver L, Baczynska M, Adams CO, Portlock T, Richardson K, Shaw R, Oregioni A, Mastroianni G, Whittaker SB, Kelly G, Lorenz CD, Fornili A, Cianciotto NP, Garnett JA
RgGuinier 2.8 nm
Dmax 9.5 nm
VolumePorod 104 nm3

SASDUR7 – Infectious Bursal Disease Virus VP3 (central domains at pH 8.0)

UniProt ID: P61825 (844-976) Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012)
Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) experimental SAS data
Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) Kratky plot
Sample: Structural polyprotein (Capsid protein VP3: K947R; Δ756-843; Δ977-1012) monomer, 18 kDa Infectious bursal disease … protein
Buffer: 50 mM TRIS, 500 mM NaCl, 2 mM DTT,, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 2
Infectious Bursal Disease Virus VP3
Diego Ferrero
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 21 nm3

SASDUS7 – Infectious Bursal Disease Virus VP3 (C-terminal deleted at pH 8.0)

UniProt ID: P61825 (756-976) Structural polyprotein (Capsid protein VP3: K947R; Δ977-1012)
Structural polyprotein (Capsid protein VP3: K947R; Δ977-1012) experimental SAS data
Structural polyprotein (Capsid protein VP3: K947R; Δ977-1012) Kratky plot
Sample: Structural polyprotein (Capsid protein VP3: K947R; Δ977-1012) dimer, 55 kDa Infectious bursal disease … protein
Buffer: 50 mM TRIS, 500 mM NaCl, 2 mM DTT, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 1
Infectious Bursal Disease Virus VP3
Diego Ferrero
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 110 nm3

SASDUT7 – The tandem SH2 domains of Tyrosine-protein kinase SYK

UniProt ID: P43405 (6-269) Tyrosine-protein kinase SYK
Tyrosine-protein kinase SYK experimental SAS data
Tyrosine-protein kinase SYK Kratky plot
Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13
The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures Structure (2024)
Bradshaw W, Harris G, Gileadi O, Katis V
RgGuinier 2.2 nm
Dmax 6.9 nm
VolumePorod 49 nm3

SASDUU7 – The tandem SH2 domains of Tyrosine-protein kinase SYK with a bound FCER1G diphospho-ITAM peptide

UniProt ID: P43405 (6-269) Tyrosine-protein kinase SYK

UniProt ID: P30273 (62-81) High affinity immunoglobulin epsilon receptor subunit gamma
Tyrosine-protein kinase SYKHigh affinity immunoglobulin epsilon receptor subunit gamma experimental SAS data
Tyrosine-protein kinase SYK High affinity immunoglobulin epsilon receptor subunit gamma Kratky plot
Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein
High affinity immunoglobulin epsilon receptor subunit gamma monomer, 2 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13
The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures Structure (2024)
Bradshaw W, Harris G, Gileadi O, Katis V
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 54 nm3

SASDUV7 – The tandem SH2 domains of Tyrosine-protein kinase SYK with a bound CD3G diphospho-ITAM peptide

UniProt ID: P43405 (6-269) Tyrosine-protein kinase SYK

UniProt ID: P09693 (157-176) T-cell surface glycoprotein CD3 gamma chain
Tyrosine-protein kinase SYKT-cell surface glycoprotein CD3 gamma chain experimental SAS data
Tyrosine-protein kinase SYK T-cell surface glycoprotein CD3 gamma chain Kratky plot
Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein
T-cell surface glycoprotein CD3 gamma chain monomer, 3 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13
The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures Structure (2024)
Bradshaw W, Harris G, Gileadi O, Katis V
RgGuinier 2.1 nm
Dmax 7.1 nm
VolumePorod 56 nm3

SASDUW7 – The tandem SH2 domains of Tyrosine-protein kinase SYK with a bound TYROBP diphospho-ITAM peptide

UniProt ID: P43405 (6-269) Tyrosine-protein kinase SYK

UniProt ID: O43914 (88-107) TYRO protein tyrosine kinase-binding protein
Tyrosine-protein kinase SYKTYRO protein tyrosine kinase-binding protein experimental SAS data
Tyrosine-protein kinase SYK TYRO protein tyrosine kinase-binding protein Kratky plot
Sample: Tyrosine-protein kinase SYK monomer, 30 kDa Homo sapiens protein
TYRO protein tyrosine kinase-binding protein monomer, 2 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Nov 13
The mechanism of allosteric activation of SYK kinase derived from multiple phospho-ITAM-bound structures Structure (2024)
Bradshaw W, Harris G, Gileadi O, Katis V
RgGuinier 2.1 nm
Dmax 7.1 nm
VolumePorod 53 nm3

SASDUX7 – Aspergillus niger beta-glucosidase (bgl1), partially deglycosylated with Endoglycosidase H

UniProt ID: Q9P8F4 (1-860) beta-glucosidase
beta-glucosidase experimental SAS data
GASBOR model
Sample: Beta-glucosidase dimer, 187 kDa Aspergillus niger protein
Buffer: 50 mM sodium acetate, pH: 5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2024 Feb 15
Aspergillus niger beta-glucosidase (bgl1)
Estella Yee
RgGuinier 4.2 nm
Dmax 14.0 nm
VolumePorod 253 nm3

SASDU78 – Trypanosoma brucei ESAG4 membrane-proximal Venus Fly Trap domain 2 (VFT2)

UniProt ID: Q8WPR6 (480-859) adenylate cyclase
adenylate cyclase experimental SAS data
ALPHAFOLD model
Sample: Adenylate cyclase monomer, 43 kDa Trypanosoma brucei protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Sep 23
Biophysical analysis of the membrane-proximal Venus Flytrap domain of ESAG4 receptor-like adenylate cyclase from Trypanosoma brucei. Mol Biochem Parasitol 260:111653 (2024)
Alves DO, Geens R, da Silva Arruda HR, Jennen L, Corthaut S, Wuyts E, de Andrade GC, Prosdocimi F, Cordeiro Y, Pires JR, Vieira LR, de Oliveira GAP, Sterckx YG, Salmon D
RgGuinier 2.3 nm
Dmax 7.6 nm
VolumePorod 76 nm3

SASDU88 – SEC-SAXS of the fusion protein MPB-MabR(Rv2242) - monomeric peak

UniProt ID: P0AEX9 (27-384) Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A)

UniProt ID: P9WPH5 (19-409) Uncharacterized protein Rv2242
Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A)Uncharacterized protein Rv2242 experimental SAS data
ALPHAFOLD model
Sample: Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) monomer, 40 kDa Escherichia coli (strain … protein
Uncharacterized protein Rv2242 monomer, 43 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, pH:
Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 3
Domain architecture of the Mycobacterium tuberculosis MabR (Rv2242), a member of the PucR transcription factor family Heliyon :e40494 (2024)
Megalizzi V, Tanina A, Grosse C, Mirgaux M, Legrand P, Mirandela G, Wohlkönig A, Bifani P, Wintjens R
RgGuinier 2.8 nm
Dmax 8.5 nm
VolumePorod 113 nm3