SASBDB entries for UniProt ID:

SASDME9 – 7SK stem-loop 1 with HEXIM Arginine Rich Motif

UniProt ID: None (None-None) Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA

UniProt ID: O94992 (146-156) Protein HEXIM1
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNAProtein HEXIM1 experimental SAS data
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Protein HEXIM1 Kratky plot
Sample: Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA monomer, 18 kDa Homo sapiens RNA
Protein HEXIM1 monomer, 2 kDa Homo sapiens protein
Buffer: 10 mM phosphate, 70 mM NaCl, 0.1 mM EDTA, pH: 5.6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 27
A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA. Commun Biol 5(1):819 (2022)
Pham VV, Gao M, Meagher JL, Smith JL, D'Souza VM
RgGuinier 2.2 nm
Dmax 8.2 nm
VolumePorod 26 nm3

SASDNN3 – Peptidyl-prolyl cis-trans isomerase FKBP43, nucleoplasmin domain (amino acids 1-96)

UniProt ID: F4J9Q6 (1-96) Peptidyl-prolyl cis-trans isomerase FKBP43
Peptidyl-prolyl cis-trans isomerase FKBP43 experimental SAS data
ALPHAFOLD model
Sample: Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 58 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris, 300 mM NaCl, 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 16
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
Singh AK, Saharan K, Baral S, Vasudevan D
RgGuinier 2.5 nm
Dmax 7.3 nm
VolumePorod 141 nm3

SASDNV7 – Obscurin Ig domains 11/12 at neutral pH

UniProt ID: Q5VST9 (981-1162) Obscurin
Obscurin experimental SAS data
Obscurin Kratky plot
Sample: Obscurin monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Nov 18
The N-terminus of obscurin is flexible in solution. Proteins (2022)
Mauriello GE, Moncure GE, Nowzari RA, Miller CJ, Wright NT
RgGuinier 3.7 nm
Dmax 18.0 nm
VolumePorod 66 nm3

SASDN78 – Pikachurin N-terminal FnIII(1-2) fragment (SEC-SAXS)

UniProt ID: Q63HQ2 (25-237) Pikachurin N-terminal FnIII(1-2) domains
Pikachurin N-terminal FnIII(1-2) domains experimental SAS data
GASBOR model
Sample: Pikachurin N-terminal FnIII(1-2) domains monomer, 25 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 3
Structure of the photoreceptor synaptic assembly of the extracellular matrix protein pikachurin with the orphan receptor GPR179 Science Signaling 16(795) (2023)
Patil D, Pantalone S, Cao Y, Laboute T, Novick S, Singh S, Savino S, Faravelli S, Magnani F, Griffin P, Singh A, Forneris F, Martemyanov K
RgGuinier 3.4 nm
Dmax 16.4 nm
VolumePorod 44 nm3

SASDNE8 – apo-Pyrococcus furiosus argonaute protein at 10 °C

UniProt ID: Q8U3D2 (5-770) Piwi domain-containing protein
Piwi domain-containing protein experimental SAS data
GASBOR model
Sample: Piwi domain-containing protein monomer, 91 kDa Pyrococcus furiosus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 142 nm3

SASDNL8 – The C-terminal region of histone-lysine N-methyltransferase NSD3: PWWP2-SET construct

UniProt ID: Q9BZ95 (942-1318) Histone-lysine N-methyltransferase NSD3
Histone-lysine N-methyltransferase NSD3 experimental SAS data
OTHER model
Sample: Histone-lysine N-methyltransferase NSD3 monomer, 43 kDa Homo sapiens protein
Buffer: 0.5 M NaCl, 20 mM Tris-HCl, 5 mM DTT, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Nov 25
Structural insights into the C-terminus of the histone-lysine N-methyltransferase NSD3 by small-angle X-ray scattering. Front Mol Biosci 11:1191246 (2024)
Belviso BD, Shen Y, Carrozzini B, Morishita M, di Luccio E, Caliandro R
RgGuinier 3.1 nm
Dmax 11.2 nm
VolumePorod 64 nm3

SASDNW9 – Glucose-regulated protein 78, nucleotide-binding domain

UniProt ID: P11021 (26-405) Endoplasmic reticulum chaperone BiP
Endoplasmic reticulum chaperone BiP experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endoplasmic reticulum chaperone BiP monomer, 42 kDa Homo sapiens protein
Buffer: phosphate buffered saline, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 21
Structural basis of CDNF interaction with the UPR regulator GRP78. Nat Commun 15(1):8175 (2024)
Graewert MA, Volkova M, Jonasson K, Määttä JAE, Gräwert T, Mamidi S, Kulesskaya N, Evenäs J, Johnsson RE, Svergun D, Bhattacharjee A, Huttunen HJ
RgGuinier 2.2 nm
Dmax 6.5 nm
VolumePorod 70 nm3

SASDNY9 – dark-adapted Orange Carotenoid Protein-wtCtag at 0.7 mg/mL

UniProt ID: P74102 (2-317) Orange carotenoid-binding protein
Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
Andreeva EA, Niziński S, Wilson A, Levantino M, De Zitter E, Munro R, Muzzopappa F, Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.4 nm
Dmax 6.7 nm
VolumePorod 55 nm3

SASDPA4 – SAXS data for the trimeric C-phycocyanin

UniProt ID: P72509 (1-162) C-phycocyanin alpha subunit

UniProt ID: P72508 (1-172) C-phycocyanin beta subunit
C-phycocyanin alpha subunitC-phycocyanin beta subunit experimental SAS data
GASBOR model
Sample: C-phycocyanin alpha subunit trimer, 53 kDa Arthrospira platensis protein
C-phycocyanin beta subunit trimer, 54 kDa Arthrospira platensis protein
Buffer: 150 mM NaCl, 20 mM Tris, pH: 7.6
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Apr 14
Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores Structural Dynamics 9(5):054701 (2022)
Tsoraev G, Protasova E, Klimanova E, Ryzhykau Y, Kuklin A, Semenov Y, Ge B, Li W, Qin S, Friedrich T, Sluchanko N, Maksimov E
RgGuinier 3.9 nm
Dmax 13.2 nm
VolumePorod 154 nm3

SASDPE4 – P2X7 ballast domain

UniProt ID: Q99572 (395-595) P2X purinoceptor 7
P2X purinoceptor 7 experimental SAS data
P2X purinoceptor 7 Kratky plot
Sample: P2X purinoceptor 7 trimer, 74 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4
New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H
RgGuinier 3.1 nm
Dmax 10.5 nm
VolumePorod 94 nm3