SASBDB entries for UniProt ID:

SASDF66 – DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 4.5 with 300 mM NaCl

UniProt ID: None (None-None) 80bp_DNA Forward

UniProt ID: None (None-None) 80bp_DNA Reverse

UniProt ID: P0ACF0 (1-90) DNA-binding protein HU-alpha

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
DNA-binding protein HU-alpha, 10 kDa Escherichia coli protein
Buffer: 10 mM sodium acetate, 300 mM NaCl, pH: 4.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Jun 1
Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDGH6 – Ubiquitin activating enzyme 5 (1.0 mg/ml)

UniProt ID: Q9GZZ9 (57-346) Ubiquitin-like modifier-activating enzyme 5

Ubiquitin-like modifier-activating enzyme 5 experimental SAS data
SASREF model
Sample: Ubiquitin-like modifier-activating enzyme 5 dimer, 68 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 13
Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism Journal of Structural Biology :107796 (2021)
Fuchs S, Kikhney A, Schubert R, Kaiser C, Liebau E, Svergun D, Betzel C, Perbandt M
RgGuinier 2.9 nm
Dmax 10.0 nm
VolumePorod 82 nm3

SASDKG7 – Bromodomain-containing protein 4, BRD4, tandem bromodomains (2 mg/ml)

UniProt ID: O60885 (38-460) Bromodomain-containing protein 4

Bromodomain-containing protein 4 experimental SAS data
Bromodomain-containing protein 4 Kratky plot
Sample: Bromodomain-containing protein 4 monomer, 48 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 25
Multivalent nucleosome scaffolding by bromodomain and extraterminal domain tandem bromodomains. J Biol Chem :108289 (2025)
Olp MD, Bursch KL, Wynia-Smith SL, Nuñez R, Goetz CJ, Jackson V, Smith BC
RgGuinier 5.2 nm
Dmax 20.1 nm
VolumePorod 100 nm3

SASDLZ3 – Human Albumin (C6) control SIGMA

UniProt ID: P02768 (None-None) Albumin

Albumin experimental SAS data
SREFLEX model
Sample: Albumin monomer, 69 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM KCl, 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 1
Albumin in patients with liver disease shows an altered conformation. Commun Biol 4(1):731 (2021)
Paar M, Fengler VH, Rosenberg DJ, Krebs A, Stauber RE, Oettl K, Hammel M
RgGuinier 2.8 nm

SASDL78 – The Fe–S cluster assembly 1 homolog of pigeon (retention time at the magnet position No.4 : 10 min)

UniProt ID: P0DN75 (2-132) Iron-sulfur cluster assembly 1 homolog, mitochondrial

Iron-sulfur cluster assembly 1 homolog, mitochondrial experimental SAS data
Iron-sulfur cluster assembly 1 homolog, mitochondrial Kratky plot
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial, 15 kDa Columba livia protein
Buffer: 20 mM Tris-HCl, 0.15 M NaCl, 10 mM 3-mercapto-1,2-propanediol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2020 Nov 30
Magnetic field effects on the structure and molecular behavior of pigeon iron–sulfur protein Protein Science 31(6) (2022)
Arai S, Shimizu R, Adachi M, Hirai M
RgGuinier 4.9 nm
Dmax 16.5 nm

SASDP92 – light-adapted Orange Carotenoid Protein-wtCtag at 3.5 mg/mL

UniProt ID: P74102 (2-317) Orange carotenoid-binding protein

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
Andreeva EA, Niziński S, Wilson A, Levantino M, De Zitter E, Munro R, Muzzopappa F, Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 3.4 nm
Dmax 17.0 nm
VolumePorod 78 nm3

SASDR33 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) of SARS-CoV-2 in HEPES conditions

UniProt ID: P0DTC9 (44-180) Nucleoprotein

Nucleoprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements. Nat Commun 14(1):3331 (2023)
Korn SM, Dhamotharan K, Jeffries CM, Schlundt A
RgGuinier 1.6 nm
Dmax 5.2 nm
VolumePorod 26 nm3

SASDRD5 – A ssr1698 Dri1 hemoprotein, H79A:R90A variant + heme

UniProt ID: P73129 (1-96) Ssr1698 protein (H79A:R90A)

Ssr1698 protein (H79A:R90A) experimental SAS data
Sample: Ssr1698 protein (H79A:R90A) dimer, 22 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Mar 30
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
Grosjean N, Yee EF, Kumaran D, Chopra K, Abernathy M, Biswas S, Byrnes J, Kreitler DF, Cheng JF, Ghosh A, Almo SC, Iwai M, Niyogi KK, Pakrasi HB, Sarangi R, van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.3 nm
VolumePorod 28 nm3

SASDSU9 – SARS-CoV-2 N-protein (N1-245, residues 1-245): 400 µM

UniProt ID: P0DTC9 (1-245) Nucleoprotein

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 110 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez
RgGuinier 5.4 nm
Dmax 24.0 nm
VolumePorod 157 nm3

SASDT93 – Teneurin-3 A0B1 isoform - 0.65 mg/mL

UniProt ID: Q9WTS6-3 (343-2706) Isoform A0B1 of Teneurin-3

Isoform A0B1 of Teneurin-3 experimental SAS data
Isoform A0B1 of Teneurin-3 Kratky plot
Sample: Isoform A0B1 of Teneurin-3 dimer, 545 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Sep 10
Alternative splicing controls teneurin-3 compact dimer formation for neuronal recognition Nature Communications 15(1) (2024)
Gogou C, Beugelink J, Frias C, Kresik L, Jaroszynska N, Drescher U, Janssen B, Hindges R, Meijer D
RgGuinier 9.4 nm
Dmax 33.5 nm
VolumePorod 1032 nm3