SASBDB entries for UniProt ID:

SASDDZ9 – Protein translocase subunit SecA (amino acids 1-880)

UniProt ID: P10408 (None-None) Protein translocase subunit SecA
Protein translocase subunit SecA experimental SAS data
Protein translocase subunit SecA Kratky plot
Sample: Protein translocase subunit SecA dimer, 199 kDa Escherichia coli protein
Buffer: 20mM HEPES, 100mM NaCl, 1mM TCEP, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 18
The C-terminal tail of the bacterial translocation ATPase SecA modulates its activity. Elife 8 (2019)
Jamshad M, Knowles TJ, White SA, Ward DG, Mohammed F, Rahman KF, Wynne M, Hughes GW, Kramer G, Bukau B, Huber D
RgGuinier 4.2 nm
Dmax 14.8 nm
VolumePorod 380 nm3

SASDEQ3 – Senescence-associated E3 ubiquitin ligase 1, 5.6 mg/ml

UniProt ID: Q9LM76 (None-None) Senescence-associated E3 ubiquitin ligase 1
Senescence-associated E3 ubiquitin ligase 1 experimental SAS data
SASREF MX model
Sample: Senescence-associated E3 ubiquitin ligase 1 tetramer, 355 kDa Arabidopsis thaliana protein
Buffer: 50 mM Tris, 250 mM NaCl, pH: 9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Senescence-associated ubiquitin ligase 1 (SAUL1)
Haifa El Kilani, Al Kikhney
RgGuinier 5.9 nm
Dmax 22.0 nm

SASDER4 – Wild-type suppressor of copper sensitivity C protein, PmScsC, with concentration series data

UniProt ID: A0A1Z1SYD5 (22-243) Suppressor of Copper Sensitivity C protein
Suppressor of Copper Sensitivity C protein experimental SAS data
OTHER model
Sample: Suppressor of Copper Sensitivity C protein trimer, 74 kDa Proteus mirabilis protein
Buffer: 10mM HEPES, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 5
Engineered variants provide new insight into the structural properties important for activity of the highly dynamic, trimeric protein disulfide isomerase ScsC from Proteus mirabilis. Acta Crystallogr D Struct Biol 75(Pt 3):296-307 (2019)
Furlong EJ, Kurth F, Premkumar L, Whitten AE, Martin JL
RgGuinier 3.7 nm
Dmax 11.1 nm
VolumePorod 101 nm3

SASDEW5 – Albumin-insulin detemir 1:6 complex, binding in Südlow's Site II

UniProt ID: P01308 (25-110) Insulin detemir (Levemir(R), Novo Nordisk A/S)

UniProt ID: P02768 (25-609) Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.)
Insulin detemir (Levemir(R), Novo Nordisk A/S)Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) experimental SAS data
SASREF CV model
Sample: Insulin detemir (Levemir(R), Novo Nordisk A/S) hexamer, 35 kDa protein
Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Buffer: 6.9 mM Na2HPO4, 11.9 mM m-cresol, 13.7 mM phenol, 157.3 mM glycerol, 38.5 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 162 nm3

SASDEC6 – Adenylation Domain of DNA ligase A with NAD+

UniProt ID: P9WNV1 (2-328) DNA ligase A
DNA ligase A experimental SAS data
DAMMIF model
Sample: DNA ligase A monomer, 38 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Oct 6
Salt bridges at the subdomain interfaces of the adenylation domain and active-site residues of Mycobacterium tuberculosis NAD + -dependent DNA ligase A (MtbLigA) are important for the initial steps of nick-sealing activity Acta Crystallographica Section D Structural Biology 77(6) (2021)
Afsar M, Shukla A, Kumar N, Ramachandran R
RgGuinier 2.4 nm
Dmax 6.2 nm
VolumePorod 94 nm3

SASDEP7 – Filamin A Ig-like domains 3-5 P637Q mutant (FLNa3-5 P637Q)

UniProt ID: P21333 (478-766) Filamin A Ig.like domains 3-5 P637Q mutant
Filamin A Ig.like domains 3-5 P637Q mutant experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Filamin A Ig.like domains 3-5 P637Q mutant monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 10
Non-syndromic Mitral Valve Dysplasia Mutation Changes the Force Resilience and Interaction of Human Filamin A. Structure (2018)
Haataja TJK, Bernardi RC, Lecointe S, Capoulade R, Merot J, Pentikäinen U
RgGuinier 2.2 nm
Dmax 7.4 nm
VolumePorod 39 nm3

SASDER7 – KRAB-associated protein 1 (KAP1); TRIM28, amino acids 23-812

UniProt ID: Q13263 (23-812) Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 experimental SAS data
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 Kratky plot
Sample: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 dimer, 175 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 6
KAP1 is an antiparallel dimer with a functional asymmetry. Life Sci Alliance 2(4) (2019)
Fonti G, Marcaida MJ, Bryan LC, Träger S, Kalantzi AS, Helleboid PJ, Demurtas D, Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M
RgGuinier 8.9 nm
Dmax 38.0 nm
VolumePorod 853 nm3

SASDES7 – Stator protein complex FlaG/FlaF

UniProt ID: Q4J9K8 (35-164) Conserved flagellar protein F

UniProt ID: Q4J9K7 (32-151) Stator protein FlaG soluble domain
Conserved flagellar protein FStator protein FlaG soluble domain experimental SAS data
MULTIFOXS model
Sample: Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein
Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 109 nm3

SASDEV8 – Shwachman-Bodian-Diamond Syndrome protein (yeast SDO1)

UniProt ID: Q07953 (None-None) Ribosome maturation protein SDO1
Ribosome maturation protein SDO1 experimental SAS data
MULTIFOXS model
Sample: Ribosome maturation protein SDO1 monomer, 28 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl, 5 mM MgCl2., pH:
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21
Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations. Int J Mol Sci 19(12) (2018)
Gijsbers A, Montagut DC, Méndez-Godoy A, Altamura D, Saviano M, Siliqi D, Sánchez-Puig N
RgGuinier 2.7 nm
Dmax 8.5 nm

SASDE69 – Interleukin-1 receptor type 2, IL-1RII-ECD

UniProt ID: P27930 (14-341) Interleukin-1 receptor type 2
Interleukin-1 receptor type 2 experimental SAS data
BILBOMD model
Sample: Interleukin-1 receptor type 2 monomer, 38 kDa Homo sapiens protein
Buffer: 10mM HEPES, 150mM NaCl, 3% glycerol, pH: 7.2
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jul 24
Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling. Structure 27(8):1296-1307.e5 (2019)
Ge J, Remesh SG, Hammel M, Pan S, Mahan AD, Wang S, Wang X
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 80 nm3