Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDAK6 – Glucose Isomerase

UniProt ID: P24300 (None-None) Xylose isomerase

Sample:	Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer:	PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Apr 24

Standard proteins
Erica Valentini

R_g^Guinier	3.4	nm
D_max	9.7	nm
Volume^Porod	293	nm³

SASDAL6 – Wild-type chalcone isomerase, ligand-free

UniProt ID: V9P0A9 (None-None) Bacterial chalcone isomerase

Bacterial chalcone isomerase experimental SAS data

Bacterial chalcone isomerase Kratky plot

Sample:	Bacterial chalcone isomerase hexamer, 194 kDa Eubacterium ramulus protein
Buffer:	50 mM sodium phosphate, pH: 6.8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23

Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase. Acta Crystallogr D Biol Crystallogr 71(Pt 4):907-17 (2015)
Thomsen M, Tuukkanen A, Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W

R_g^Guinier	4.0	nm
D_max	13.0	nm
Volume^Porod	320	nm³

SASDAM6 – Naringenin-bound chalcone isomerase

UniProt ID: V9P0A9 (None-None) Chalcone isomerase with Naringenin

Chalcone isomerase with Naringenin experimental SAS data

Chalcone isomerase with Naringenin Kratky plot

Sample:	Chalcone isomerase with Naringenin hexamer, 194 kDa Eubacterium ramulus protein
Buffer:	50 mM sodium phosphate, pH: 6.8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23

R_g^Guinier	3.7	nm
D_max	11.0	nm
Volume^Porod	320	nm³

SASDAN6 – Chalcone isomerase, CHI_Δlid construct

UniProt ID: V9P0A9 (None-None) Chalcone isomerase deltaLid

Chalcone isomerase deltaLid experimental SAS data

Sample:	Chalcone isomerase deltaLid hexamer, 181 kDa Eubacterium ramulus protein
Buffer:	50 mM sodium phosphate, pH: 6.8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23

R_g^Guinier	3.6	nm
D_max	11.0	nm
Volume^Porod	270	nm³

SASDAS6 – Plectin, fragment of the plakin domain encompassing the spectrin repeats SR3-SR4-SR5 and the SH3

UniProt ID: Q15149 (None-None) Plectin

Sample:	Plectin monomer, 43 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 2.5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at cSAXS, Swiss Light Source on 2009 Jul 24

The structure of the plakin domain of plectin reveals a non-canonical SH3 domain interacting with its fourth spectrin repeat. J Biol Chem 286(14):12429-38 (2011)
Ortega E, Buey RM, Sonnenberg A, de Pereda JM

R_g^Guinier	4.4	nm
D_max	14.5	nm
Volume^Porod	57	nm³

SASDAT6 – Integrin beta4, fragment of the cytoplasmic region encompassing the third and fourth FnIII domains

UniProt ID: P16144 (None-None) Integrin beta-4

Sample:	Integrin beta-4 monomer, 23 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 6

Combination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin α6β4. Acta Crystallogr D Biol Crystallogr 71(Pt 4):969-85 (2015)
Alonso-García N, García-Rubio I, Manso JA, Buey RM, Urien H, Sonnenberg A, Jeschke G, de Pereda JM

R_g^Guinier	2.2	nm
D_max	7.0	nm
Volume^Porod	32	nm³

SASDAV6 – Cysteine desulfurase IscS dimer

UniProt ID: P0A6B7 (None-None) Cysteine desulfurase IscS

Cysteine desulfurase IscS experimental SAS data

Sample:	Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 5

Structural bases for the interaction of frataxin with the central components of iron-sulphur cluster assembly. Nat Commun 1:95 (2010)
Prischi F, Konarev PV, Iannuzzi C, Pastore C, Adinolfi S, Martin SR, Svergun DI, Pastore A

R_g^Guinier	3.2	nm
D_max	10.9	nm

SASDAW6 – Iron-sulfur cluster assembly scaffold IscU monomer

UniProt ID: P0ACD4 (None-None) Iron-sulfur cluster assembly scaffold protein IscU

Iron-sulfur cluster assembly scaffold protein IscU experimental SAS data

Sample:	Iron-sulfur cluster assembly scaffold protein IscU monomer, 14 kDa Escherichia coli protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 5

R_g^Guinier	1.9	nm
D_max	6.4	nm

SASDAX6 – Protein CyaY monomer

UniProt ID: P27838 (None-None) Protein CyaY

Sample:	Protein CyaY monomer, 12 kDa Escherichia coli protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 5

R_g^Guinier	1.5	nm
D_max	5.0	nm

SASDAY6 – IcsS-dimer and IscU-dimer complex

UniProt ID: P0A6B7 (None-None) Cysteine desulfurase IscS

UniProt ID: P0ACD4 (None-None) Iron-sulfur cluster assembly scaffold protein IscU

Cysteine desulfurase IscSIron-sulfur cluster assembly scaffold protein IscU experimental SAS data

Sample:	Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein Iron-sulfur cluster assembly scaffold protein IscU dimer, 29 kDa Escherichia coli protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 5

R_g^Guinier	3.6	nm
D_max	12.1	nm

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