SASBDB entries for UniProt ID:

SASDA35 – SeZinT-SeZnuA complex

UniProt ID: V2JZU2 (None-None) High-affinity zinc transporter periplasmic component

UniProt ID: L6R5I5 (None-None) Zinc/cadmium-binding protein
High-affinity zinc transporter periplasmic componentZinc/cadmium-binding protein experimental SAS data
BUNCH model
Sample: High-affinity zinc transporter periplasmic component monomer, 33 kDa Salmonella enterica subsp. … protein
Zinc/cadmium-binding protein monomer, 23 kDa Salmonella enterica subsp. … protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2013 May 9
The Salmonella enterica ZinT structure, zinc affinity and interaction with the high-affinity uptake protein ZnuA provide insight into the management of periplasmic zinc. Biochim Biophys Acta 1840(1):535-44 (2014)
Ilari A, Alaleona F, Tria G, Petrarca P, Battistoni A, Zamparelli C, Verzili D, Falconi M, Chiancone E
RgGuinier 2.5 nm
Dmax 8.5 nm
VolumePorod 55 nm3

SASDA45 – PpoA wild type enzyme

UniProt ID: Q6RET3 (None-None) Psi-producing oxygenase A
Psi-producing oxygenase A experimental SAS data
SASREF model
Sample: Psi-producing oxygenase A trimer, 362 kDa Aspergillus nidulans protein
Buffer: 20 Mm HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jun 24
A structural model of PpoA derived from SAXS-analysis-implications for substrate conversion. Biochim Biophys Acta 1831(9):1449-57 (2013)
Koch C, Tria G, Fielding AJ, Brodhun F, Valerius O, Feussner K, Braus GH, Svergun DI, Bennati M, Feussner I
RgGuinier 5.4 nm
Dmax 16.5 nm
VolumePorod 540 nm3

SASDAK5 – Myomesin-1 My12-My13

UniProt ID: P52179 (1225-1443) Myomesin-1
Myomesin-1 experimental SAS data
CRYSOL model
Sample: Myomesin-1 dimer, 46 kDa Homo sapiens protein
Buffer: 25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 May 7
Molecular basis of the C-terminal tail-to-tail assembly of the sarcomeric filament protein myomesin. EMBO J 27(1):253-64 (2008)
Pinotsis N, Lange S, Perriard JC, Svergun DI, Wilmanns M
RgGuinier 4.0 nm
Dmax 14.5 nm
VolumePorod 56 nm3

SASDAZ5 – NetrinVIV

UniProt ID: O95631 (None-None) Netrin-1
Netrin-1 experimental SAS data
DAMMIN model
Sample: Netrin-1 monomer, 49 kDa Homo sapiens protein
Buffer: 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 26
The crystal structure of netrin-1 in complex with DCC reveals the bifunctionality of netrin-1 as a guidance cue. Neuron 83(4):839-849 (2014)
Finci LI, Krüger N, Sun X, Zhang J, Chegkazi M, Wu Y, Schenk G, Mertens HDT, Svergun DI, Zhang Y, Wang JH, Meijers R
RgGuinier 3.9 nm
Dmax 13.5 nm
VolumePorod 100 nm3

SASDA76 – NetrinVIV DCC56 complex

UniProt ID: O95631 (None-None) Netrin-1

UniProt ID: (None-None) Deleted in Colorectal Cancer (FN5 & FN6)
Netrin-1Deleted in Colorectal Cancer (FN5 & FN6) experimental SAS data
Netrin-1 Deleted in Colorectal Cancer (FN5 & FN6) Kratky plot
Sample: Netrin-1 monomer, 49 kDa Homo sapiens protein
Deleted in Colorectal Cancer (FN5 & FN6) monomer, 26 kDa Homo sapiens protein
Buffer: 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
The crystal structure of netrin-1 in complex with DCC reveals the bifunctionality of netrin-1 as a guidance cue. Neuron 83(4):839-849 (2014)
Finci LI, Krüger N, Sun X, Zhang J, Chegkazi M, Wu Y, Schenk G, Mertens HDT, Svergun DI, Zhang Y, Wang JH, Meijers R
RgGuinier 5.1 nm
Dmax 17.0 nm
VolumePorod 120 nm3

SASDA86 – NetrinVIV DCC56(M933R) complex

UniProt ID: O95631 (None-None) Netrin-1

UniProt ID: (None-None) Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant
Netrin-1Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant experimental SAS data
Netrin-1 Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant Kratky plot
Sample: Netrin-1 monomer, 49 kDa Homo sapiens protein
Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant monomer, 26 kDa Homo sapiens protein
Buffer: 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 26
The crystal structure of netrin-1 in complex with DCC reveals the bifunctionality of netrin-1 as a guidance cue. Neuron 83(4):839-849 (2014)
Finci LI, Krüger N, Sun X, Zhang J, Chegkazi M, Wu Y, Schenk G, Mertens HDT, Svergun DI, Zhang Y, Wang JH, Meijers R
RgGuinier 4.0 nm
Dmax 14.0 nm
VolumePorod 95 nm3

SASDA96 – Lysozyme

UniProt ID: P00698 (19-147) Lysozyme C
Lysozyme C experimental SAS data
DAMMIF model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 20 mM Sodium Acetate/HEPES, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Feb 17
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 24 nm3

SASDAA6 – Human serum albumin monomer and mixtures

UniProt ID: P02768 (25-609) Human serum albumin monomer
Human serum albumin monomer experimental SAS data
DAMMIF model
Sample: Human serum albumin monomer monomer, 66 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 22
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 2.8 nm
Dmax 8.4 nm
VolumePorod 103 nm3

SASDAF6 – K1K2K3 domain of Kgp gingipain

UniProt ID: Q51817 (982-1662) K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain
K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain experimental SAS data
BUNCH model
Sample: K1K2K3 adhesin modules of lysine-specific (Kgp) gingipain monomer, 74 kDa Porphyromonas gingivalis W83 protein
Buffer: 10 mM TRIS 150 mM NaCl, pH: 7.6
Experiment: SAXS data collected at Anton Paar SAXSess, University of Sydney on 2010 Aug 15
The modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis. Mol Microbiol 81(5):1358-73 (2011)
Li N, Yun P, Jeffries CM, Langley D, Gamsjaeger R, Church WB, Hunter N, Collyer CA
RgGuinier 4.3 nm
Dmax 14.7 nm

SASDAG6 – K1K2 domains of Kgp gingipain

UniProt ID: Q51817 (982-1334) K1K2 adhesin modules of lysine-specific (Kgp) gingipain
K1K2 adhesin modules of lysine-specific (Kgp) gingipain experimental SAS data
BUNCH model
Sample: K1K2 adhesin modules of lysine-specific (Kgp) gingipain monomer, 38 kDa Porphyromonas gingivalis W83 protein
Buffer: 10 mM TRIS 150 mM NaCl, pH: 7.6
Experiment: SAXS data collected at Anton Paar SAXSess, University of Sydney on 2010 Aug 15
The modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis. Mol Microbiol 81(5):1358-73 (2011)
Li N, Yun P, Jeffries CM, Langley D, Gamsjaeger R, Church WB, Hunter N, Collyer CA
RgGuinier 2.9 nm
Dmax 9.5 nm