SASBDB entries for UniProt ID:

SASDEW4 – ACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc

UniProt ID: None (None-None) Membrane scaffold protein 1D1 (deuterated, 75%)

UniProt ID: None (None-None) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)

UniProt ID: Q9LF79 (None-None) Calcium-transporting ATPase 8, plasma membrane-type

UniProt ID: P59220 (None-None) Calmodulin-7
Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-typeCalmodulin-7 experimental SAS data
Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Calcium-transporting ATPase 8, plasma membrane-type Calmodulin-7 Kratky plot
Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli
Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein
Calmodulin-7 monomer, Arabidopsis thaliana protein
Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Commun Biol 1:206 (2018)
Nitsche J, Josts I, Heidemann J, Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H
RgGuinier 5.9 nm
Dmax 22.0 nm
VolumePorod 805 nm3

SASDEX4 – Herpes simplex virus 1 tegument protein UL11

UniProt ID: P04289 (1-96) Cytoplasmic envelopment protein 3
Cytoplasmic envelopment protein 3 experimental SAS data
Cytoplasmic envelopment protein 3 Kratky plot
Sample: Cytoplasmic envelopment protein 3 monomer, 12 kDa Human alphaherpesvirus 1 protein
Buffer: 50 mM HEPES, 100 mM NaCl, 0.5 mM tris(2-carboxyethyl)phosphine (TCEP),, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Jun 4
Conserved Outer Tegument Component UL11 from Herpes Simplex Virus 1 Is an Intrinsically Disordered, RNA-Binding Protein. mBio 11(3) (2020)
Metrick CM, Koenigsberg AL, Heldwein EE
RgGuinier 2.4 nm
Dmax 12.0 nm
VolumePorod 30 nm3

SASDEY4 – Ribonuclease A monomer from in-house SEC-SAXS

UniProt ID: P61823 (27-150) Ribonuclease pancreatic
Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 10 nm3

SASDEZ4 – Carbonic Anhydrase from in-house SEC-SAXS

UniProt ID: P00921 (None-None) Carbonic anhydrase 2
Carbonic anhydrase 2 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 38 nm3

SASDE25 – Human insulin hexamer from in-house SEC-SAXS

UniProt ID: P01308 (25-110) Human insulin
Human insulin experimental SAS data
DAMFILT model
Sample: Human insulin hexamer, 35 kDa Homo sapiens protein
Buffer: 7 mM sodium phosphate, 60 mM phenol, 200 µM Zn(CH3COO)2, 23 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 26
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.0 nm
Dmax 5.9 nm
VolumePorod 39 nm3

SASDE35 – Ovalbumin monomer from in-house SEC-SAXS

UniProt ID: P01012 (None-None) Ovalbumin
Ovalbumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 55 nm3

SASDE45 – Conalbumin monomer from in-house SEC-SAXS

UniProt ID: P02789 (20-705) Ovotransferrin
Ovotransferrin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ovotransferrin monomer, 76 kDa Gallus gallus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 3.0 nm
Dmax 10.1 nm
VolumePorod 100 nm3

SASDE55 – Apoferritin monomer from in-house SEC-SAXS

UniProt ID: P02791 (None-None) Ferritin light chain
Ferritin light chain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ferritin light chain 24-mer, 476 kDa Equus caballus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 5.1 nm
Dmax 12.3 nm
VolumePorod 685 nm3

SASDE65 – Bovine serum albumin monomer from in-house SEC-SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 92 nm3

SASDE75 – Bovine serum albumin monomer from in-house SEC-SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 104 nm3