SASBDB entries for UniProt ID: P02769

SASDA32 – Bovine serum albumin in HEPES

UniProt ID: None (2-222) Bovine Serum Albumin
Bovine Serum Albumin experimental SAS data
DAMMIF model
Sample: Bovine Serum Albumin monomer, 69 kDa Bos taurus (Bovine) protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 12
Standard proteins
Darja Ruskule
RgGuinier 2.9 nm
Dmax 9.3 nm
VolumePorod 97 nm3

SASDBJ3 – Bovine serum albumin, monomer from SEC-SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin, monomer
Bovine serum albumin, monomer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 100 nm3

SASDBK3 – Bovine serum albumin, dimer from SEC-SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin, dimer
Bovine serum albumin, dimer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin, dimer dimer, 133 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 3.9 nm
Dmax 12.7 nm
VolumePorod 202 nm3

SASDBT4 – Bovine serum albumin monomer (measured by SEC-SAXS)

UniProt ID: P02769 (25-607) Bovine serum albumin, monomer
Bovine serum albumin, monomer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer: 20 mM Tris 142 mM NaCl 5 % Glycerol 1mM DTT, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 22
Bovine Serum Albumin measured by SEC-SAXS
Martha Brennich
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 118 nm3

SASDCJ3 – Bovine Serum Albumin

UniProt ID: P02769 (25-607) Serum albumin
Serum albumin experimental SAS data
DAMMIN model
Sample: Serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, Tainer JA, Vachette P, Westbrook J, Whitten AE
RgGuinier 2.8 nm
Dmax 8.7 nm
VolumePorod 101 nm3

SASDCF8 – Bovine serum albumin (WAXS)

UniProt ID: P02769 (25-607) Serum albumin
Serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Serum albumin monomer, 66 kDa Bos taurus protein
Buffer: Hepes, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 25
WAXS benchmark on standard proteins
Maxim Petoukhov
RgGuinier 2.7 nm

SASDCQ8 – Bovine serum albumin (BSA)

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: TRIS 50mM, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 2.8 nm

SASDDN3 – Bovine serum albumin mixture: averaged and individual data frames (subtracted and unsubtracted test sets)

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
Bovine serum albumin Kratky plot
Sample: Bovine serum albumin, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Sep 25
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 117 nm3

SASDE65 – Bovine serum albumin monomer from in-house SEC-SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 92 nm3

SASDE75 – Bovine serum albumin monomer from in-house SEC-SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 104 nm3