SASBDB entries for UniProt ID: P02791

SASDA82 – Apoferritin in PBS and glycerol

UniProt ID: P02791 (None-None) Ferritin light chain
Ferritin light chain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer: PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 20
Standard proteins
Darja Ruskule
RgGuinier 6.8 nm
Dmax 12.8 nm
VolumePorod 619 nm3

SASDCD8 – Horse spleen apoferritin (WAXS)

UniProt ID: P02791 (None-None) Horse spleen apoferritin
Horse spleen apoferritin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Horse spleen apoferritin 24-mer, 476 kDa Equus caballus protein
Buffer: tbs, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jul 15
WAXS benchmark on standard proteins
Maxim Petoukhov
RgGuinier 6.3 nm

SASDE55 – Apoferritin monomer from in-house SEC-SAXS

UniProt ID: P02791 (None-None) Ferritin light chain
Ferritin light chain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ferritin light chain 24-mer, 476 kDa Equus caballus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 5.1 nm
Dmax 12.3 nm
VolumePorod 685 nm3

SASDQZ8 – Apoferritin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

UniProt ID: P02791 (1-175) Ferritin light chain
Ferritin light chain experimental SAS data
Ferritin light chain Kratky plot
Sample: Ferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SANS data collected at SPB/SFX, European XFEL on 2021 May 14
Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wollweber T, Zhuang Y, Schulz J, Haas H, Chapman HN, Mancuso AP, Svergun D
RgGuinier 5.8 nm

SASDWC3 – Apoferritin, from SEC-SANS

UniProt ID: P02791 (2-175) Ferritin light chain
Ferritin light chain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ferritin light chain 24-mer, 476 kDa Equus caballus protein
Buffer: 100 mM Tris-HCl, 100 mM NaCl in 100% v/v D₂O, pH: 7.5
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Apr 22
Size-exclusion chromatography–small-angle neutron scattering system optimized for an instrument with medium neutron flux Journal of Applied Crystallography 58(2) (2025)
Morishima K, Inoue R, Nakagawa T, Shimizu M, Sakamoto R, Oda T, Mayumi K, Sugiyama M
RgGuinier 5.4 nm
Dmax 23.1 nm

SASDW94 – Horse apoferritin from AF4-SAXS measurements

UniProt ID: P02791 (1-175) Ferritin light chain
Ferritin light chain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ferritin light chain 24-mer, 480 kDa Equus caballus protein
Buffer: phosphate buffered saline, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19
AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline. J Synchrotron Radiat (2025)
Da Vela S, Bartels K, Franke D, Soloviov D, Gräwert T, Molodenskiy D, Kolb B, Wilhelmy C, Drexel R, Meier F, Haas H, Langguth P, Graewert MA
RgGuinier 5.4 nm
Dmax 13.3 nm
VolumePorod 632 nm3

SASDFN8 – Apoferritin from horse spleen - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

UniProt ID: P02791 (1-175) Apoferritin light chain
Apoferritin light chain experimental SAS data
DAMMIN model
Sample: Apoferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 5.4 nm
Dmax 12.5 nm
VolumePorod 679 nm3