Small Angle Scattering Biological Data Bank SASBDB

Browse by DISSEMINATION: Published

SASDWA7 – Dihydroneopterin aldolase from H.pylori (HpDHNA:Pterin)

Dihydroneopterin aldolase experimental SAS data

Sample:	Dihydroneopterin aldolase tetramer, 55 kDa Helicobacter pylori (strain … protein
Buffer:	25 mM Tris-HCl pH 7.5 and 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Apr 5

Structure of Helicobacter pylori dihydroneopterin aldolase suggests a fragment-based strategy for isozyme-specific inhibitor design. Curr Res Struct Biol 5:100095 (2023)
Shaw GX, Fan L, Cherry S, Shi G, Tropea JE, Ji X

R_g^Guinier	2.5	nm
D_max	7.3	nm
Volume^Porod	77	nm³

SASDQT5 – NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) wild-type

Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 experimental SAS data

Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 Kratky plot

Sample:	Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20

Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA. Nucleic Acids Res (2022)
Keil P, Wulf A, Kachariya N, Reuscher S, Hühn K, Silbern I, Altmüller J, Keller M, Stehle R, Zarnack K, Sattler M, Urlaub H, Sträßer K

R_g^Guinier	2.0	nm
D_max	6.8	nm
Volume^Porod	24	nm³

SASDQU5 – NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) P196D/A197D linker mutant

Sample:	Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20

R_g^Guinier	1.9	nm
D_max	6.7	nm
Volume^Porod	25	nm³

SASDQV5 – NPL3 protein RNA recognition motifs 1 and 2 (RRM1,2) wild-type, bound to RNA

Serine/arginine (SR)-type shuttling mRNA binding protein NPL3Short RNA oligonucleotide (NPL3 binding sequence) experimental SAS data

Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 Short RNA oligonucleotide (NPL3 binding sequence) Kratky plot

Sample:	Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 monomer, 18 kDa Saccharomyces cerevisiae (strain … protein Short RNA oligonucleotide (NPL3 binding sequence) monomer, 4 kDa RNA
Buffer:	20 mM NaPO4, 50 mM NaCl, 1 mM DTT, pH: 6.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2020 Feb 20

R_g^Guinier	2.0	nm
D_max	6.3	nm
Volume^Porod	30	nm³

SASDQB7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region)

Non-structural protein V experimental SAS data

Sample:	Non-structural protein V monomer, 15 kDa Hendra virus (isolate … protein
Buffer:	50 mM sodium phosphate, pH: 7.2
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Jul 14

Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
Nilsson J, Baroudi H, Gondelaud F, Pesce G, Bignon C, Ptchelkine D, Chamieh J, Cottet H, Kajava A, Longhi S

R_g^Guinier	3.7	nm
D_max	19.0	nm
Volume^Porod	48	nm³

SASDQC7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region) mutated YYY --> AAA

Non-structural protein V (Y211A, Y212A, Y213A mutant) experimental SAS data

Non-structural protein V (Y211A, Y212A, Y213A mutant) Kratky plot

Sample:	Non-structural protein V (Y211A, Y212A, Y213A mutant) monomer, 15 kDa Hendra virus (isolate … protein
Buffer:	50 mM sodium phosphate, pH: 7.2
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Jul 14

R_g^Guinier	4.0	nm
D_max	19.0	nm
Volume^Porod	49	nm³

SASDQD7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region, C-terminal truncated)

Sample:	Non-structural protein V monomer, 9 kDa Hendra virus (isolate … protein
Buffer:	50 mM sodium phosphate, pH: 7.2
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Jul 14

R_g^Guinier	2.8	nm
D_max	13.0	nm
Volume^Porod	22	nm³

SASDQE7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region C-terminal truncated), mutated YYY --> AAA

Non-structural protein V (ΔC-terminal and Y111A, Y112A, Y113A mutant) experimental SAS data

Non-structural protein V (ΔC-terminal and Y111A, Y112A, Y113A mutant) Kratky plot

Sample:	Non-structural protein V (ΔC-terminal and Y111A, Y112A, Y113A mutant) monomer, 9 kDa Hendra virus (isolate … protein
Buffer:	50 mM sodium phosphate, pH: 7.2
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Jul 14

R_g^Guinier	2.7	nm
D_max	12.5	nm
Volume^Porod	21	nm³

SASDQF7 – Region within N-terminal domain of P/V/W protein of Nipah virus (PNT3 region)

Sample:	Non-structural protein V monomer, 15 kDa Nipah henipavirus protein
Buffer:	50 mM sodium phosphate, pH: 7.2
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Jul 14

R_g^Guinier	3.7	nm
D_max	16.5	nm
Volume^Porod	47	nm³

SASDPV2 – original TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

Fusion protein of LSm and MyoX-coil experimental SAS data

Sample:	Fusion protein of LSm and MyoX-coil, 1066 kDa Artificial protein protein
Buffer:	25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 May 23

Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions. J Am Chem Soc (2022)
Ohara N, Kawakami N, Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K

R_g^Guinier	9.1	nm
D_max	22.7	nm

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