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SASDRE2 – Gamma cyclodextrin - 25 mg/ml aqueous solution measured to 238 inverse nm

Gamma Cyclodextrin experimental SAS data
Gamma Cyclodextrin Kratky plot
Sample: Gamma Cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.7 nm

SASDRF2 – Gamma cyclodextrin - 12.5 mg/ml aqueous solution measured to 238 inverse nm

Gamma Cyclodextrin experimental SAS data
Gamma Cyclodextrin Kratky plot
Sample: Gamma Cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.7 nm

SASDPL2 – Fused complex of cytochrome P450 143 and ferredoxin Rv1786 (Cyp143 and rv1786)

His-tagged fused complex of cytochrome P450 143 and ferredoxin Rv1786 experimental SAS data
Fused complex of cytochrome P450 143 and ferredoxin Rv1786 (Cyp143 and rv1786) Rg histogram
Sample: His-tagged fused complex of cytochrome P450 143 and ferredoxin Rv1786 monomer, 54 kDa Mycobacterium tuberculosis protein
Buffer: 300 mM NaCl, 50 mM Tris/TrisHCl, 10% glycerol, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 7
Structural insights into 3Fe–4S ferredoxins diversity in M. tuberculosis highlighted by a first redox complex with P450 Frontiers in Molecular Biosciences 9 (2023)
Gilep A, Varaksa T, Bukhdruker S, Kavaleuski A, Ryzhykau Y, Smolskaya S, Sushko T, Tsumoto K, Grabovec I, Kapranov I, Okhrimenko I, Marin E, Shevtsov M, Mishin A, Kovalev K, Kuklin A, Gordeliy V, Kaluzhskiy L, Gnedenko O, Yablokov E, Ivanov A, Borshchevskiy V, Strushkevich N
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 85 nm3

SASDQ37 – N-terminal GST-like domain of Plasmodium berghei cytosolic glutamyl-tRNA synthetase

Glutamate--tRNA ligase experimental SAS data
OLIGOMER model
Sample: Glutamate--tRNA ligase dimer, 57 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 3.0 nm
Dmax 11.5 nm
VolumePorod 96 nm3

SASDQ47 – N-terminal GST-like domain of Plasmodium berghei tRNA import protein

tRNA import protein tRIP experimental SAS data
CORAL model
Sample: TRNA import protein tRIP dimer, 49 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 2.9 nm
Dmax 11.0 nm
VolumePorod 68 nm3

SASDQ57 – The scaffold of Plasmodium berghei multi aminoacyl-tRNA synthetase complexes

Chimeric construct containing the GST-like domains of P. berghei tRNA import protein and glutamyl-tRNA synthetase experimental SAS data
CORAL model
Sample: Chimeric construct containing the GST-like domains of P. berghei tRNA import protein and glutamyl-tRNA synthetase dimer, 105 kDa Plasmodium berghei ANKA protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 3.9 nm
Dmax 14.1 nm
VolumePorod 145 nm3

SASDQ67 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, Q-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and glutaminyl-tRNA synthetase

Glutamine--tRNA ligaseGlutamate--tRNA ligasetRNA import protein tRIP experimental SAS data
CORAL model
Sample: Glutamine--tRNA ligase dimer, 46 kDa Plasmodium berghei (strain … protein
Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
TRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 4.6 nm
Dmax 17.1 nm
VolumePorod 198 nm3

SASDQ77 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, M-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and methionyl-tRNA synthetase

Methionine--tRNA ligasetRNA import protein tRIPGlutamate--tRNA ligase experimental SAS data
CORAL model
Sample: Methionine--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
TRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein
Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
Jaramillo Ponce JR, Théobald-Dietrich A, Bénas P, Paulus C, Sauter C, Frugier M
RgGuinier 4.7 nm
Dmax 18.3 nm
VolumePorod 217 nm3

SASDM45 – TrYbe® trispecific antibody

Tri-specific antibody experimental SAS data
OTHER model
Sample: Tri-specific antibody monomer, 102 kDa Homo sapiens protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 26
TRYBE®: an Fc-free antibody format with three monovalent targeting arms engineered for long in vivo half-life. MAbs 15(1):2160229 (2023)
Davé E, Durrant O, Dhami N, Compson J, Broadbridge J, Archer S, Maroof A, Whale K, Menochet K, Bonnaillie P, Barry E, Wild G, Peerboom C, Bhatta P, Ellis M, Hinchliffe M, Humphreys DP, Heywood SP
RgGuinier 4.1 nm
Dmax 13.0 nm
VolumePorod 157 nm3

SASDP89 – IgG Fc receptor CD64

High affinity immunoglobulin gamma Fc recombinant CD64 experimental SAS data
High affinity immunoglobulin gamma Fc recombinant CD64 Kratky plot
Sample: High affinity immunoglobulin gamma Fc recombinant CD64 monomer, 32 kDa synthetic construct protein
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Dec 9
The solution structure of the unbound IgG Fc receptor CD64 resembles its crystal structure: Implications for function. PLoS One 18(9):e0288351 (2023)
Hui GK, Gao X, Gor J, Lu J, Sun PD, Perkins SJ
RgGuinier 3.4 nm
Dmax 12.3 nm
VolumePorod 30 nm3