Browse by DISSEMINATION: Published

SASDP27 – BTB domain of CG6765 protein at 1.5 mg/ml

Uncharacterized protein, isoform A experimental SAS data
ALPHAFOLD model
Sample: Uncharacterized protein, isoform A hexamer, 92 kDa Drosophila melanogaster protein
Buffer: 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 8
BTB domains: A structural view of evolution, multimerization, and protein-protein interactions. Bioessays :e2200179 (2022)
Bonchuk A, Balagurov K, Georgiev P
RgGuinier 3.7 nm
Dmax 15.0 nm
VolumePorod 167 nm3

SASDP37 – BTB domain of CG6765 protein at 3.0 mg/ml

Uncharacterized protein, isoform A experimental SAS data
Uncharacterized protein, isoform A Kratky plot
Sample: Uncharacterized protein, isoform A hexamer, 92 kDa Drosophila melanogaster protein
Buffer: 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 8
BTB domains: A structural view of evolution, multimerization, and protein-protein interactions. Bioessays :e2200179 (2022)
Bonchuk A, Balagurov K, Georgiev P
RgGuinier 4.1 nm
Dmax 17.0 nm
VolumePorod 192 nm3

SASDQ48 – Minimal proline dehydrogenase domain of proline utilization A (SmPutADeltaAlpha2) 1.1 mg/mL

Minimal proline dehydrogenase domain of proline utilization A (design #2) experimental SAS data
OTHER model
Sample: Minimal proline dehydrogenase domain of proline utilization A (design #2) dimer, 87 kDa Sinorhizobium meliloti protein
Buffer: 25 mM HEPES pH 7.6, 150 mM NaCl, and 1mM TCEP, pH: 7.6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 12
Structure-based engineering of minimal Proline dehydrogenase domains for inhibitor discovery. Protein Eng Des Sel (2022)
Bogner AN, Ji J, Tanner JJ
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 102 nm3

SASDQ58 – Minimal proline dehydrogenase domain of proline utilization A (SmPutADeltaAlpha2) 2.3 mg/mL

Minimal proline dehydrogenase domain of proline utilization A (design #2) experimental SAS data
OTHER model
Sample: Minimal proline dehydrogenase domain of proline utilization A (design #2) dimer, 87 kDa Sinorhizobium meliloti protein
Buffer: 25 mM HEPES pH 7.6, 150 mM NaCl, and 1mM TCEP, pH: 7.6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 12
Structure-based engineering of minimal Proline dehydrogenase domains for inhibitor discovery. Protein Eng Des Sel (2022)
Bogner AN, Ji J, Tanner JJ
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 102 nm3

SASDQ68 – Minimal proline dehydrogenase domain of proline utilization A (SmPutADeltaAlpha2) 3.4 mg/mL

Minimal proline dehydrogenase domain of proline utilization A (design #2) experimental SAS data
OTHER model
Sample: Minimal proline dehydrogenase domain of proline utilization A (design #2) dimer, 87 kDa Sinorhizobium meliloti protein
Buffer: 25 mM HEPES pH 7.6, 150 mM NaCl, and 1mM TCEP, pH: 7.6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2022 Apr 12
Structure-based engineering of minimal Proline dehydrogenase domains for inhibitor discovery. Protein Eng Des Sel (2022)
Bogner AN, Ji J, Tanner JJ
RgGuinier 3.0 nm
Dmax 9.8 nm
VolumePorod 108 nm3

SASDP47 – Glucose Isomerase at a neutral pH and 5 MPa (1 atm) of pressure

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 25 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 3.4 nm
Dmax 10.6 nm
VolumePorod 253 nm3

SASDP57 – Glucose Isomerase at a neutral pH and 100 MPa (1000 atm) of pressure

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 3% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 3.5 nm
Dmax 10.6 nm
VolumePorod 267 nm3

SASDP67 – Bovine serum albumin at neutral pH, 4 °C, and 5 MPa (1 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.7 nm
Dmax 8.5 nm
VolumePorod 99 nm3

SASDP77 – Bovine serum albumin at neutral pH, 4 °C, and 100 MPa (1000 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.6 nm
Dmax 8.3 nm
VolumePorod 94 nm3

SASDP87 – Bovine serum albumin at neutral pH, 50 °C, and 5 MPa (1 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 3.0 nm
Dmax 9.5 nm
VolumePorod 108 nm3