Small Angle Scattering Biological Data Bank SASBDB

Browse by DISSEMINATION: Published

SASDNS6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex at 1.4 mg/ml

Retinoblastoma-associated proteinEarly E1A protein experimental SAS data

Retinoblastoma-associated protein Early E1A protein Kratky plot

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

Conformational buffering underlies functional selection in intrinsically disordered protein regions. Nat Struct Mol Biol (2022)
González-Foutel NS, Glavina J, Borcherds WM, Safranchik M, Barrera-Vilarmau S, Sagar A, Estaña A, Barozet A, Garrone NA, Fernandez-Ballester G, Blanes-Mira C, Sánchez IE, de Prat-Gay G, Cortés J, Bernadó P, Pappu RV, Holehouse AS, Daughdrill GW, Chemes LB

R_g^Guinier	3.0	nm
D_max	12.0	nm
Volume^Porod	84	nm³

SASDNT6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex at 2.7 mg/ml

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	3.3	nm
D_max	17.0	nm
Volume^Porod	93	nm³

SASDNU6 – Retinoblastoma protein/Early E1A protein (RB-E1A) complex - merged data from concentration series measurements

Sample:	Retinoblastoma-associated protein monomer, 41 kDa Homo sapiens protein Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 14

R_g^Guinier	3.0	nm
D_max	12.0	nm
Volume^Porod	84	nm³

SASDNV6 – Early E1A protein, SEC-SAXS data

Sample:	Early E1A protein monomer, 13 kDa Human adenovirus C … protein
Buffer:	20 mM sodium phosphate pH 7.0, 200 mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 24

R_g^Guinier	3.6	nm
D_max	17.5	nm
Volume^Porod	45	nm³

SASDNX3 – Calcium-bound Calmodulin, including structural models

Calcium-bound Calmodulin, including structural models Rg histogram

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Nov 15

Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium. BMC Biol 20(1):176 (2022)
Léger C, Pitard I, Sadi M, Carvalho N, Brier S, Mechaly A, Raoux-Barbot D, Davi M, Hoos S, Weber P, Vachette P, Durand D, Haouz A, Guijarro JI, Ladant D, Chenal A

R_g^Guinier	2.2	nm
D_max	7.2	nm
Volume^Porod	33	nm³

SASDNY3 – Calcium-bound Calmodulin complexed with Calmidazolium

Calmodulin-1Calmidazolium experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Calmidazolium monomer, 1 kDa
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Nov 15

R_g^Guinier	1.7	nm
D_max	5.2	nm
Volume^Porod	30	nm³

SASDNT5 – Bradyzoite Pseudokinase 1 (amino acids 61-377)

Rhoptry kinase family protein experimental SAS data

Sample:	Rhoptry kinase family protein tetramer, 143 kDa Toxoplasma gondii (strain … protein
Buffer:	20 mM HEPES, 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 19

Divergent kinase WNG1 is regulated by phosphorylation of an atypical activation sub-domain. Biochem J (2022)
Dewangan PS, Beraki TG, Paiz EA, Appiah Mensah D, Chen Z, Reese ML

R_g^Guinier	3.8	nm
D_max	11.5	nm
Volume^Porod	207	nm³

SASDHB5 – RelA Homology Domain of p50/RelA heterodimer

NF-kappa-B p105 subunit 39-350Transcription factor p65 19-321 experimental SAS data

Sample:	NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein Transcription factor p65 19-321 monomer, 35 kDa Mus musculus protein
Buffer:	25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11

An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
Baughman HER, Narang D, Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA

R_g^Guinier	3.8	nm
D_max	13.0	nm
Volume^Porod	110	nm³

SASDHC5 – Nuclear factor kB p50/RelA heterodimer

Transcription factor p65 19-549NF-kappa-B p105 subunit 39-350 experimental SAS data

Sample:	Transcription factor p65 19-549 monomer, 58 kDa Mus musculus protein NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
Buffer:	25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11

R_g^Guinier	4.6	nm
D_max	15.3	nm
Volume^Porod	183	nm³

SASDHD5 – Transactivation domain of RelA

Transcription factor p65 340-549 experimental SAS data

Sample:	Transcription factor p65 340-549 monomer, 23 kDa Mus musculus protein
Buffer:	25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11

R_g^Guinier	2.7	nm
D_max	8.2	nm
Volume^Porod	59	nm³

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