Small Angle Scattering Biological Data Bank SASBDB

Browse by DISSEMINATION: Published

SASDM22 – Staphylococcus aureus lipid II isoglutaminyl synthase MurT:GatD complex

Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurTLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD experimental SAS data

Staphylococcus aureus lipid II isoglutaminyl synthase MurT:GatD complex Rg histogram

Sample:	Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT monomer, 49 kDa Staphylococcus aureus (strain … protein Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD monomer, 28 kDa Staphylococcus aureus (strain … protein
Buffer:	100 mM Tris-HCl, 500 mM NaCl, 10 mM MgCl2, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Nov 29

Unravelling the reaction mechanism of glutamate amidation in Staphylococcus aureus peptidoglycan PhD thesis, NOVA University Lisbon - (2022)
Francisco Miguel Piçarra Leisico, Mertens HD

R_g^Guinier	3.1	nm
D_max	10.5	nm
Volume^Porod	121	nm³

SASDLS7 – Mycobacterium phage TipsytheTRex immunity repressor bound to DNA

Immunity repressor21mer dsDNA experimental SAS data

Sample:	Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein 21mer dsDNA monomer, 13 kDa DNA
Buffer:	20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10

A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence. Nat Commun 13(1):4105 (2022)
McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR

R_g^Guinier	2.3	nm
D_max	7.4	nm
Volume^Porod	41	nm³

SASDMK3 – Mycobacterium phage TipsytheTRex apo protein

Immunity repressor experimental SAS data

Sample:	Immunity repressor monomer, 24 kDa Mycobacterium phage TipsytheTRex protein
Buffer:	20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10

R_g^Guinier	2.3	nm
D_max	9.0	nm
Volume^Porod	43	nm³

SASDML3 – Mycobacterium phage TipsytheTRex immunity repressor bound to 24mer DNA

Immunity repressor24mer dsDNA experimental SAS data

Sample:	Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein 24mer dsDNA monomer, 15 kDa DNA
Buffer:	20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10

R_g^Guinier	2.4	nm
D_max	9.0	nm
Volume^Porod	43	nm³

SASDMM3 – Mycobacterium phage TipsytheTRex immunity repressor mixed with 13mer DNA

Immunity repressor13mer dsDNA experimental SAS data

Sample:	Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein 13mer dsDNA monomer, 8 kDa DNA
Buffer:	20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 10

R_g^Guinier	2.0	nm
D_max	8.5	nm
Volume^Porod	18	nm³

SASDNP3 – Recombinant phospholipase A2 receptor at pH 7.2

Secretory phospholipase A2 receptor experimental SAS data

Secretory phospholipase A2 receptor Kratky plot

Sample:	Secretory phospholipase A2 receptor monomer, 189 kDa Homo sapiens protein
Buffer:	10 mM Bis-Tris, 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 19

Structure of PLA2R reveals presentation of the dominant membranous nephropathy epitope and an immunogenic patch Proceedings of the National Academy of Sciences 119(29) (2022)
Fresquet M, Lockhart-Cairns M, Rhoden S, Jowitt T, Briggs D, Baldock C, Brenchley P, Lennon R

R_g^Guinier	5.9	nm
D_max	26.0	nm
Volume^Porod	504	nm³

SASDNQ3 – Recombinant phospholipase A2 receptor at pH 6.2

Sample:	Secretory phospholipase A2 receptor monomer, 189 kDa Homo sapiens protein
Buffer:	10 mM Bis-Tris, 150 mM NaCl, pH: 6.2
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 19

R_g^Guinier	4.2	nm
D_max	15.8	nm
Volume^Porod	426	nm³

SASDLF4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S mutant

ChiLob 7/4 IgG2 F(ab)2 C224S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C224S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

Hinge disulfides in human IgG2 CD40 antibodies modulate receptor signaling by regulation of conformation and flexibility Science Immunology 7(73) (2022)
Orr C, Fisher H, Yu X, Chan C, Gao Y, Duriez P, Booth S, Elliott I, Inzhelevskaya T, Mockridge I, Penfold C, Wagner A, Glennie M, White A, Essex J, Pearson A, Cragg M, Tews I

R_g^Guinier	3.9	nm
D_max	14.2	nm
Volume^Porod	135	nm³

SASDLG4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S+C225S mutant

ChiLob 7/4 IgG2 F(ab)2 C224S/C225S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S+C225S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C224S/C225S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

R_g^Guinier	4.2	nm
D_max	15.1	nm
Volume^Porod	135	nm³

SASDLH4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S mutant

ChiLob 7/4 IgG2 F(ab)2 C225S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C225S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

R_g^Guinier	4.0	nm
D_max	14.5	nm
Volume^Porod	131	nm³

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