Small Angle Scattering Biological Data Bank SASBDB

Browse by DISSEMINATION: Published

SASDLJ4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C228S mutant

ChiLob 7/4 IgG2 F(ab)2 C228S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C228S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C228S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

Hinge disulfides in human IgG2 CD40 antibodies modulate receptor signaling by regulation of conformation and flexibility Science Immunology 7(73) (2022)
Orr C, Fisher H, Yu X, Chan C, Gao Y, Duriez P, Booth S, Elliott I, Inzhelevskaya T, Mockridge I, Penfold C, Wagner A, Glennie M, White A, Essex J, Pearson A, Cragg M, Tews I

R_g^Guinier	4.1	nm
D_max	13.5	nm
Volume^Porod	135	nm³

SASDLK4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S, kappa chain C214S mutant

ChiLob 7/4 IgG2 F(ab)2 C224S/kC214S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S, kappa chain C214S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C224S/kC214S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

R_g^Guinier	4.0	nm
D_max	13.3	nm
Volume^Porod	133	nm³

SASDLL4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S, kappa chain C214S mutant

ChiLob 7/4 IgG2 F(ab)2 C225S/kC214S mutant experimental SAS data

Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S, kappa chain C214S mutant Rg histogram

Sample:	ChiLob 7/4 IgG2 F(ab)2 C225S/kC214S mutant monomer, 99 kDa protein
Buffer:	50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 9

R_g^Guinier	4.0	nm
D_max	13.7	nm
Volume^Porod	130	nm³

SASDJX7 – Labrum-Interacting Proteins from Saliva 2 (34k-2) - SEC-SAXS

Similar to Aedes aegypti 34 kDa salivary secreted protein 34k-2 experimental SAS data

Sample:	Similar to Aedes aegypti 34 kDa salivary secreted protein 34k-2 monomer, 35 kDa Aedes albopictus protein
Buffer:	25 mM HEPES, 0.1 M NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2016 Sep 3

A salivary factor binds a cuticular protein and modulates biting by inducing morphological changes in the mosquito labrum. Curr Biol (2022)
Arnoldi I, Mancini G, Fumagalli M, Gastaldi D, D'Andrea L, Bandi C, Di Venere M, Iadarola P, Forneris F, Gabrieli P

R_g^Guinier	2.7	nm
D_max	8.4	nm
Volume^Porod	50	nm³

SASDLC9 – 2-aminomuconic 6-semialdehyde dehydrogenase double truncation mutant (Δ124-138; Δ477-491)

2-aminomuconic 6-semialdehyde dehydrogenase experimental SAS data

Sample:	2-aminomuconic 6-semialdehyde dehydrogenase dimer, 101 kDa Pseudomonas sp. protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1

The tetrameric assembly of 2-aminomuconic 6-semialdehyde dehydrogenase is a functional requirement of cofactor NAD(+) binding. Environ Microbiol 24(7):2994-3012 (2022)
Shi Q, Chen Y, Li X, Dong H, Chen C, Zhong Z, Yang C, Liu G, Su D

R_g^Guinier	2.9	nm
D_max	9.0	nm
Volume^Porod	117	nm³

SASDLD9 – 2-aminomuconic 6-semialdehyde dehydrogenase wild-type

Sample:	2-aminomuconic 6-semialdehyde dehydrogenase tetramer, 215 kDa Pseudomonas sp. protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1

R_g^Guinier	3.9	nm
D_max	11.6	nm
Volume^Porod	326	nm³

SASDNC5 – Acinetobacter baumannii riboflavin biosynthesis protein RibD

Riboflavin biosynthesis protein RibD experimental SAS data

Sample:	Riboflavin biosynthesis protein RibD dimer, 80 kDa Acinetobacter baumannii protein
Buffer:	150 mM NaCl, 10 mM Tris, 1 mM DTT, 5% v/v glycerol, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3

Recombinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA. Protein Expr Purif :106134 (2022)
Weber K, Doellinger J, Jeffries CM, Wilharm G

R_g^Guinier	3.7	nm
D_max	12.0	nm
Volume^Porod	115	nm³

SASDND5 – Acinetobacter baumannii aconitate hydratase B, monomer fraction

Aconitate hydratase B experimental SAS data

Sample:	Aconitate hydratase B monomer, 96 kDa Acinetobacter baumannii protein
Buffer:	100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3

R_g^Guinier	3.4	nm
D_max	12.5	nm
Volume^Porod	150	nm³

SASDNE5 – Acinetobacter baumannii aconitate hydratase B, monomer/dimer mixed fraction

Sample:	Aconitate hydratase B, 96 kDa Acinetobacter baumannii protein
Buffer:	100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3

R_g^Guinier	4.0	nm
D_max	14.0	nm
Volume^Porod	230	nm³

SASDNF5 – Acinetobacter baumannii transcriptional repressor NrdR (likely filamentous assembly)

Transcriptional repressor NrdR experimental SAS data

Transcriptional repressor NrdR Kratky plot

Sample:	Transcriptional repressor NrdR, 19 kDa Acinetobacter baumannii (strain … protein
Buffer:	100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 3

R_g^Guinier	19.9	nm
D_max	92.6	nm

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