Small Angle Scattering Biological Data Bank SASBDB

Browse by DISSEMINATION: Published

SASDPZ5 – Gcf1p protein bound to DNA at 1.0 mg/mL

Sample:	Gcf1p monomer, 26 kDa Candida albicans (strain … protein Af2_20 DNA monomer, 12 kDa DNA
Buffer:	25 mM Tris, 20 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30

Structural analysis of the Candida albicans mitochondrial DNA maintenance factor Gcf1p reveals a dynamic DNA-bridging mechanism. Nucleic Acids Res (2023)
Tarrés-Solé A, Battistini F, Gerhold JM, Piétrement O, Martínez-García B, Ruiz-López E, Lyonnais S, Bernadó P, Roca J, Orozco M, Le Cam E, Sedman J, Solà M

R_g^Guinier	3.6	nm
D_max	16.0	nm
Volume^Porod	92	nm³

SASDP26 – Gcf1p protein bound to DNA at 2.0 mg/mL

Sample:	Gcf1p monomer, 26 kDa Candida albicans (strain … protein Af2_20 DNA monomer, 12 kDa DNA
Buffer:	25 mM Tris, 20 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30

R_g^Guinier	3.9	nm
D_max	16.0	nm
Volume^Porod	133	nm³

SASDP36 – N-terminal truncated Gcf1p (amino acids 59-245) bound to DNA

Af2_20 DNAGcf1p(Δ58) experimental SAS data

Sample:	Af2_20 DNA monomer, 12 kDa DNA Gcf1p(Δ58) monomer, 22 kDa Candida albicans (strain … protein
Buffer:	25 mM Tris, 20 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Aug 30

R_g^Guinier	2.6	nm
D_max	10.5	nm
Volume^Porod	52	nm³

SASDKS8 – Escherichia coli anaerobic fatty acid beta-oxidation trifunctional enzyme subunit alpha (anEcTFE-alpha)

Fatty acid oxidation complex subunit alpha experimental SAS data

Sample:	Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli (strain … protein
Buffer:	50 mM Tris, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 9

Structural basis for different membrane-binding properties of E. coli anaerobic and human mitochondrial β-oxidation trifunctional enzymes. Structure (2023)
Sah-Teli SK, Pinkas M, Hynönen MJ, Butcher SJ, Wierenga RK, Novacek J, Venkatesan R

R_g^Guinier	3.3	nm
D_max	11.2	nm
Volume^Porod	145	nm³

SASDQJ6 – Monkeypox viral synthetic DNA fragment (DNA sequence 1, MP1)

Monekypox DNA sequence 1 experimental SAS data

Sample:	Monekypox DNA sequence 1 monomer, 6 kDa Monkeypox virus DNA
Buffer:	20 mM HEPES, 100 mM KCl, and 0.2 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Sep 23

Mapping and characterization of G‐quadruplexes in monkeypox genomes Journal of Medical Virology 95(5) (2023)
Pereira H, Gemmill D, Siddiqui M, Vasudeva G, Patel T

R_g^Guinier	1.7	nm
D_max	4.4	nm
Volume^Porod	14	nm³

SASDQK6 – Monkeypox viral synthetic DNA fragment (DNA sequence 2, MP2)

Sample:	Monekypox DNA sequence 1 monomer, 6 kDa Monkeypox virus DNA
Buffer:	20 mM HEPES, 100 mM KCl, and 0.2 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Sep 23

Mapping and characterization of G‐quadruplexes in monkeypox genomes Journal of Medical Virology 95(5) (2023)
Pereira H, Gemmill D, Siddiqui M, Vasudeva G, Patel T

R_g^Guinier	1.6	nm
D_max	4.5	nm
Volume^Porod	11	nm³

SASDQL6 – Monkeypox viral synthetic DNA fragment (DNA sequence 1, MP1 mutant)

Monekypox DNA sequence 1 mutant experimental SAS data

Sample:	Monekypox DNA sequence 1 mutant monomer, 6 kDa Monkeypox virus DNA
Buffer:	20 mM HEPES, 100 mM KCl, and 0.2 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Sep 23

Mapping and characterization of G‐quadruplexes in monkeypox genomes Journal of Medical Virology 95(5) (2023)
Pereira H, Gemmill D, Siddiqui M, Vasudeva G, Patel T

R_g^Guinier	1.9	nm
D_max	5.5	nm
Volume^Porod	16	nm³

SASDQM6 – Monkeypox viral synthetic DNA fragment (DNA sequence 2, MP2 mutant)

Monekypox DNA sequence 2 mutant experimental SAS data

Sample:	Monekypox DNA sequence 2 mutant monomer, 6 kDa Monkeypox virus DNA
Buffer:	20 mM HEPES, 100 mM KCl, and 0.2 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Sep 23

Mapping and characterization of G‐quadruplexes in monkeypox genomes Journal of Medical Virology 95(5) (2023)
Pereira H, Gemmill D, Siddiqui M, Vasudeva G, Patel T

R_g^Guinier	1.9	nm
D_max	6.0	nm
Volume^Porod	8	nm³

SASDRZ6 – D1-D2 N-terminal domains of thrombospondin type-1 domain-containing protein 7A

Thrombospondin type-1 domain-containing protein 7A experimental SAS data

ALPHAFOLD PROTEIN STRUCTURE DATABASE model

Sample:	Thrombospondin type-1 domain-containing protein 7A monomer, 17 kDa Homo sapiens protein
Buffer:	25mM Tris, 150 mM NaCl, 5 mM NaNO3, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Aug 23

Structure of the N-terminal didomain d1_d2 of the Thrombospondin type-1 domain-containing 7A (2023)
Bochel A, Mortensen S, Seifert L, Hengel F, Jeffries C, Chojnowski G, Kretz O, Huber T, Tomas N, Wilmanns M

R_g^Guinier	2.5	nm
D_max	9.5	nm
Volume^Porod	28	nm³

SASDR27 – Triple mutant (D116A, H118A, Y176A) of the D1-D2 N-terminal domains of thrombospondin type-1 domain-containing protein 7A

Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) experimental SAS data

Triple mutant (D116A, H118A, Y176A) of the D1-D2 N-terminal domains of thrombospondin type-1 domain-containing protein 7A Rg histogram

Sample:	Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) monomer, 16 kDa Homo sapiens protein
Buffer:	25mM Tris, 150 mM NaCl, 5 mM NaNO3, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Dec 2

R_g^Guinier	2.7	nm
D_max	11.5	nm
Volume^Porod	19	nm³

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