Browse by DISSEMINATION: Published

SASDP83 – Accumulation-association protein (Aap) Brpt5.5 2xH85A tetramer in the presence of ZnCl2

Accumulation associated protein (mutant) experimental SAS data
PYMOL model
Sample: Accumulation associated protein (mutant) tetramer, 312 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, 5 mM ZnCl2, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
Yarawsky AE, Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 15.7 nm
Dmax 70.0 nm

SASDP93 – K48-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
Dao TP, Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 2.6 nm
Dmax 95.0 nm
VolumePorod 44 nm3

SASDPA3 – K63-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
Dao TP, Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.2 nm
Dmax 13.0 nm
VolumePorod 44 nm3

SASDPB3 – M1-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
Dao TP, Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 46 nm3

SASDPC3 – HOTag6 tetramerization domain followed by a 10-Gly linker and monoubiquitin

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B tetramer, 53 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
Dao TP, Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 115 nm3

SASDNK9 – Monomer of the full-length apo-polyketide synthase Pks13 of Mycobacterium tuberculosis

Polyketide synthase Pks13 experimental SAS data
DAMMIN model
Sample: Polyketide synthase Pks13 monomer, 188 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Mar 1
Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L
RgGuinier 7.3 nm
Dmax 24.8 nm
VolumePorod 480 nm3

SASDNL9 – Full length apo-polyketide synthase Pks13(S1533A) with Ser801 loaded by a C16-carbon chain

Polyketide synthase Pks13 experimental SAS data
DAMMIN model
Sample: Polyketide synthase Pks13 dimer, 376 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2011 Nov 23
Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L
RgGuinier 10.3 nm
Dmax 36.0 nm
VolumePorod 2300 nm3

SASDNM9 – Thioesterase domain of polyketide synthase Pks13 at neutral pH

Polyketide synthase Pks13 experimental SAS data
DAMMIN model
Sample: Polyketide synthase Pks13 monomer, 33 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1
Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L
RgGuinier 1.9 nm
Dmax 5.5 nm
VolumePorod 56 nm3

SASDNN9 – di-domain sub fragment ACP2TE of polyketide synthase Pks13 at neutral pH

Polyketide synthase Pks13 experimental SAS data
DAMMIN model
Sample: Polyketide synthase Pks13 monomer, 62 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris-HCl 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1
Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 80 nm3

SASDNP9 – AT52: acyltransferase domain and insertion domain of polyketide synthase Pks13 at neutral pH

Polyketide synthase Pks13 experimental SAS data
DAMMIN model
Sample: Polyketide synthase Pks13 monomer, 53 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1
Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L
RgGuinier 3.5 nm
Dmax 12.0 nm
VolumePorod 115 nm3