Small Angle Scattering Biological Data Bank SASBDB

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SASDPB3 – M1-linked tetraubiquitin (Ub4)

Sample:	Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8

Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
Dao TP, Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA

R_g^Guinier	3.2	nm
D_max	12.0	nm
Volume^Porod	46	nm³

SASDPC3 – HOTag6 tetramerization domain followed by a 10-Gly linker and monoubiquitin

Sample:	Polyubiquitin-B tetramer, 53 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8

R_g^Guinier	3.8	nm
D_max	14.0	nm
Volume^Porod	115	nm³

SASDNK9 – Monomer of the full-length apo-polyketide synthase Pks13 of Mycobacterium tuberculosis

Polyketide synthase Pks13 experimental SAS data

Sample:	Polyketide synthase Pks13 monomer, 188 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Mar 1

Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L

R_g^Guinier	7.3	nm
D_max	24.8	nm
Volume^Porod	480	nm³

SASDNL9 – Full length apo-polyketide synthase Pks13(S1533A) with Ser801 loaded by a C16-carbon chain

Sample:	Polyketide synthase Pks13 dimer, 376 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2011 Nov 23

R_g^Guinier	10.3	nm
D_max	36.0	nm
Volume^Porod	2300	nm³

SASDNM9 – Thioesterase domain of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 33 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	1.9	nm
D_max	5.5	nm
Volume^Porod	56	nm³

SASDNN9 – di-domain sub fragment ACP2TE of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 62 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	3.2	nm
D_max	12.0	nm
Volume^Porod	80	nm³

SASDNP9 – AT52: acyltransferase domain and insertion domain of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 53 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	3.5	nm
D_max	12.0	nm
Volume^Porod	115	nm³

SASDNQ9 – Acyltransferase domain of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 51 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2011 Jun 3

R_g^Guinier	2.8	nm
D_max	8.0	nm
Volume^Porod	85	nm³

SASDNR9 – Subfragment ACP1KSAT tridomain of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 116 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl 50 mM NaCl, pH: 8.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	3.8	nm
D_max	12.9	nm
Volume^Porod	186	nm³

SASDNS9 – KS domain of polyketide synthase Pks13 at neutral pH

Sample:	Polyketide synthase Pks13 monomer, 51 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 Dec 7

R_g^Guinier	2.9	nm
D_max	10.0	nm
Volume^Porod	82	nm³

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