Browse by DISSEMINATION: Published

SASDLZ6 – Hexamer - monomer equilibrium of Glutamate Synthase

Glutamate synthase [NADPH] large chainGlutamate synthase [NADPH] small chain experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Glutamate synthase [NADPH] large chain hexamer, 968 kDa Azospirillum brasilense protein
Glutamate synthase [NADPH] small chain hexamer, 296 kDa Azospirillum brasilense protein
Buffer: 25 mM Hepes/KOH, 1 mM EDTA, 1 mM dithiothreitol, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Feb 19
The Subnanometer Resolution Structure of the Glutamate Synthase 1.2-MDa Hexamer by Cryoelectron Microscopy and Its Oligomerization Behavior in Solution Journal of Biological Chemistry 283(13):8237-8249 (2008)
Cottevieille M, Larquet E, Jonic S, Petoukhov M, Caprini G, Paravisi S, Svergun D, Vanoni M, Boisset N
RgGuinier 7.7 nm
Dmax 22.4 nm

SASDMS4 – Listeria monocytogenes invasion protein internalin B

Internalin B experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Internalin B monomer, 36 kDa Listeria monocytogenes serotype … protein
Buffer: 25mM Na-phosphate buffer, 150 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2003 Nov 11
X-ray and Neutron Small-Angle Scattering Analysis of the Complex Formed by the Met Receptor and the Listeria monocytogenes Invasion Protein InlB Journal of Molecular Biology 377(2):489-500 (2008)
Niemann H, Petoukhov M, Härtlein M, Moulin M, Gherardi E, Timmins P, Heinz D, Svergun D
RgGuinier 2.8 nm
Dmax 9.6 nm
VolumePorod 54 nm3

SASDMT4 – Complex of Met receptor with InlB

Hepatocyte growth factor receptorInternalin B experimental SAS data
SASREF CV model
Sample: Hepatocyte growth factor receptor monomer, 100 kDa Mus musculus protein
Internalin B monomer, 36 kDa Listeria monocytogenes serotype … protein
Buffer: 25mM Na-phosphate buffer, 150 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Mar 1
X-ray and Neutron Small-Angle Scattering Analysis of the Complex Formed by the Met Receptor and the Listeria monocytogenes Invasion Protein InlB Journal of Molecular Biology 377(2):489-500 (2008)
Niemann H, Petoukhov M, Härtlein M, Moulin M, Gherardi E, Timmins P, Heinz D, Svergun D
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 270 nm3

SASDNW2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268)

Fab fragment in complex with small molecule hapten, crystal form-1 experimental SAS data
DAMMIN model
Sample: Fab fragment in complex with small molecule hapten, crystal form-1 monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris–HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jul 25
Fab MOR03268 Triggers Absorption Shift of a Diagnostic Dye via Packaging in a Solvent-shielded Fab Dimer Interface Journal of Molecular Biology 377(1):206-219 (2008)
Hillig R, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun D, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 71 nm3

SASDNX2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268) bound to TSC ligand

Fab fragment in complex with small molecule hapten, crystal form-1(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL experimental SAS data
DAMMIN model
Sample: Fab fragment in complex with small molecule hapten, crystal form-1 monomer, 45 kDa Homo sapiens protein
(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL monomer, 0 kDa
Buffer: 20 mM Tris–HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jul 27
Fab MOR03268 Triggers Absorption Shift of a Diagnostic Dye via Packaging in a Solvent-shielded Fab Dimer Interface Journal of Molecular Biology 377(1):206-219 (2008)
Hillig R, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun D, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K
RgGuinier 4.1 nm
Dmax 16.0 nm
VolumePorod 139 nm3

SASDNY2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268) with TSC ligand excess

Fab fragment in complex with small molecule hapten, crystal form-1(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Fab fragment in complex with small molecule hapten, crystal form-1 monomer, 45 kDa Homo sapiens protein
(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL monomer, 0 kDa
Buffer: 20 mM Tris–HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jul 25
Fab MOR03268 Triggers Absorption Shift of a Diagnostic Dye via Packaging in a Solvent-shielded Fab Dimer Interface Journal of Molecular Biology 377(1):206-219 (2008)
Hillig R, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun D, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K
RgGuinier 3.6 nm
Dmax 15.0 nm
VolumePorod 107 nm3

SASDAN4 – Calmodulin:peptide complex

CalmodulinC-terminal region of human myelin basic protein experimental SAS data
SASREF model
Sample: Calmodulin monomer, 17 kDa Homo sapiens protein
C-terminal region of human myelin basic protein monomer, 2 kDa Homo sapiens protein
Buffer: 25 mM Tris75 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 28
Interaction between the C-terminal region of human myelin basic protein and calmodulin: analysis of complex formation and solution structure. BMC Struct Biol 8:10 (2008)
Majava V, Petoukhov MV, Hayashi N, Pirilä P, Svergun DI, Kursula P
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 36 nm3

SASDLW5 – Cytochrome c nitrite reductase from Thioalkalivibrio nitratireducens

Cytochrome c-552 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cytochrome c-552 hexamer, 356 kDa Thioalkalivibrio nitratireducens (strain … protein
Buffer: Tris-borate buffer, pH: 8.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 May 8
Isolation and oligomeric composition of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens. Biochemistry (Mosc) 73(2):164-70 (2008)
Tikhonova TV, Slutskaya ES, Filimonenkov AA, Boyko KM, Kleimenov SY, Konarev PV, Polyakov KM, Svergun DI, Trofimov AA, Khomenkov VG, Zvyagilskaya RA, Popov VO
RgGuinier 4.8 nm

SASDAK5 – Myomesin-1 My12-My13

Myomesin-1 experimental SAS data
CRYSOL model
Sample: Myomesin-1 dimer, 46 kDa Homo sapiens protein
Buffer: 25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 May 7
Molecular basis of the C-terminal tail-to-tail assembly of the sarcomeric filament protein myomesin. EMBO J 27(1):253-64 (2008)
Pinotsis N, Lange S, Perriard JC, Svergun DI, Wilmanns M
RgGuinier 4.0 nm
Dmax 14.5 nm
VolumePorod 56 nm3

SASDNN2 – Experimental SAXS data for hemoglobin conjucted with six-seven copies of PEG dimer (Hb2) at concentration c = 21 mg/ml

Human hemoglobin conjugated with six-seven copies of 5-kDa PEG experimental SAS data
Human hemoglobin conjugated with six-seven copies of 5-kDa PEG Kratky plot
Sample: Human hemoglobin conjugated with six-seven copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein
Buffer: Ringer's lactate solution, pH: 6.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19
Solution Structure of Poly(ethylene) Glycol-Conjugated Hemoglobin Revealed by Small-Angle X-Ray Scattering: Implications for a New Oxygen Therapeutic Biophysical Journal 94(1):173-181 (2008)
Svergun D, Ekström F, Vandegriff K, Malavalli A, Baker D, Nilsson C, Winslow R
RgGuinier 3.0 nm