Browse by DISSEMINATION: Published

SASDAP8 – kLBS1-2 DNA

kLBS1-2 DNA experimental SAS data
CRYSOL model
Sample: KLBS1-2 DNA monomer, 24 kDa unidentified herpesvirus DNA
Buffer: Tris, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2013 Apr 27
KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA. Nucleic Acids Res 43(20):10039-54 (2015)
Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE
RgGuinier 4.0 nm
Dmax 16.0 nm
VolumePorod 50 nm3

SASDAQ8 – kLANA mutant dimer-tetramer mixture

ORF73 tetramerORF73 dimer experimental SAS data
NONE model
Sample: ORF73 tetramer tetramer, 63 kDa Human herpesvirus 8 protein
ORF73 dimer dimer, 32 kDa Human herpesvirus 8 protein
Buffer: 25 mM Na/K Phosphate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Jun 21
KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA. Nucleic Acids Res 43(20):10039-54 (2015)
Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 50 nm3

SASDAR8 – mLANA 124-316 mLBS1-2 8:1 complex

MHV-68 TR DNALatency-associated nuclear antigen experimental SAS data
CRYSOL model
Sample: MHV-68 TR DNA monomer, 30 kDa unidentified herpesvirus DNA
Latency-associated nuclear antigen octamer, 269 kDa Murid herpesvirus 4 protein
Buffer: 25 mM Na/K Phosphate, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Apr 27
KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA. Nucleic Acids Res 43(20):10039-54 (2015)
Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE
RgGuinier 5.8 nm
Dmax 20.0 nm
VolumePorod 475 nm3

SASDAS8 – kLANA 1008-1150 -- kLBS1-2 complex 8:2 (partially dissociated)

kLBS1-2 DNAORF73 tetramerORF73 octamerkLBS1-2 DNA two monomers experimental SAS data
NONE model
Sample: KLBS1-2 DNA monomer, 24 kDa unidentified herpesvirus DNA
ORF73 tetramer tetramer, 63 kDa Human herpesvirus 8 protein
ORF73 octamer octamer, 126 kDa Human herpesvirus 8 protein
KLBS1-2 DNA two monomers dimer, 48 kDa unidentified herpesvirus RNA
Buffer: 25 mM Na/K Phosphate, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Apr 27
KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA. Nucleic Acids Res 43(20):10039-54 (2015)
Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 250 nm3

SASDAC9 – Varkud Satellite (VS) ribozyme in Tris

Varkud Satellite (VS) ribozyme experimental SAS data
DAMMIN model
Sample: Varkud Satellite (VS) ribozyme dimer, 120 kDa Neurospora RNA
Buffer: 10 mM Tris 25 mM KCl 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Mar 6
Crystal structure of the Varkud satellite ribozyme. Nat Chem Biol 11(11):840-6 (2015)
Suslov NB, DasGupta S, Huang H, Fuller JR, Lilley DM, Rice PA, Piccirilli JA
RgGuinier 3.9 nm
Dmax 16.4 nm
VolumePorod 240 nm3

SASDAC8 – SDS hydrolase SdsA1

Pseudomonas aeruginosa SDS hydrolase SdsA1 experimental SAS data
CRYSOL model
Sample: Pseudomonas aeruginosa SDS hydrolase SdsA1 dimer, 145 kDa Pseudomonas aeruginosa protein
Buffer: 50 mM HEPES, pH: 7
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2013 Jul 22
SdsA polymorph isolation and improvement of their crystal quality using nonconventional crystallization techniques Journal of Applied Crystallography 48(5):1551-1559 (2015)
De la Mora E, Flores-Hernández E, Jakoncic J, Stojanoff V, Siliqi D, Sánchez-Puig N, Moreno A
RgGuinier 3.6 nm
Dmax 16.9 nm
VolumePorod 261 nm3

SASDGM5 – Holo-RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA)

RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) experimental SAS data
OTHER model
Sample: RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM DTT, 4 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 May 31
Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion. Sci Rep 5:14223 (2015)
O'Brien DP, Hernandez B, Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A
RgGuinier 4.4 nm
Dmax 15.5 nm
VolumePorod 89 nm3

SASDGN5 – apo-RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA)

RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) experimental SAS data
OTHER model
Sample: RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 May 31
Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion. Sci Rep 5:14223 (2015)
O'Brien DP, Hernandez B, Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A
RgGuinier 8.3 nm
Dmax 33.0 nm

SASDCH2 – Aldehyde dehydrogenase 7A1

Aldehyde dehydrogenase 7A1 (Alpha-aminoadipic semialdehyde dehydrogenase) experimental SAS data
NONE model
Sample: Aldehyde dehydrogenase 7A1 (Alpha-aminoadipic semialdehyde dehydrogenase) tetramer, 222 kDa Homo sapiens protein
Buffer: 50 mM Tris, 5% glycerol, 0.5 mM tris(3-hydroxypropyl)phosphine, 50 mM NaCl, pH: 7.8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Mar 9
Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1. Biochemistry 54(35):5513-22 (2015)
Luo M, Tanner JJ
RgGuinier 3.8 nm
Dmax 11.5 nm
VolumePorod 270 nm3

SASDAR6 – human CSF-1:CSF-1R extracellular signalling complex

Macrophage colony-stimulating factor 1Macrophage colony-stimulating factor 1 receptor experimental SAS data
SASREF model
Sample: Macrophage colony-stimulating factor 1 dimer, 35 kDa Homo sapiens protein
Macrophage colony-stimulating factor 1 receptor dimer, 107 kDa Homo sapiens protein
Buffer: 50 mM NaH2PO4, 100 m, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Mar 13
Structure and Assembly Mechanism of the Signaling Complex Mediated by Human CSF-1. Structure 23(9):1621-1631 (2015)
Felix J, De Munck S, Verstraete K, Meuris L, Callewaert N, Elegheert J, Savvides SN
RgGuinier 5.7 nm
Dmax 17.9 nm
VolumePorod 299 nm3