Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Ab initio only

SASDMX6 – 40-mer single stranded DNA inhibitor of DNA dC->dU-editing enzyme APOBEC3G

40-mer single stranded inhibitory DNA experimental SAS data

Sample:	40-mer single stranded inhibitory DNA monomer, 12 kDa DNA
Buffer:	50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 8

Small-Angle X-ray Scattering (SAXS) Measurements of APOBEC3G Provide Structural Basis for Binding of Single-Stranded DNA and Processivity Viruses 14(9):1974 (2022)
Barzak F, Ryan T, Mohammadzadeh N, Harjes S, Kvach M, Kurup H, Krause K, Chelico L, Filichev V, Harjes E, Jameson G

R_g^Guinier	3.2	nm
D_max	11.8	nm
Volume^Porod	26	nm³

SASDMY6 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length

DNA dC->dU-editing enzyme APOBEC-3G experimental SAS data

Sample:	DNA dC->dU-editing enzyme APOBEC-3G tetramer, 186 kDa Homo sapiens protein
Buffer:	50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 6

R_g^Guinier	4.2	nm
D_max	13.3	nm
Volume^Porod	350	nm³

SASDMZ6 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length in complex with DNA: tetramer

DNA dC->dU-editing enzyme APOBEC-3G40-mer single stranded inhibitory DNA experimental SAS data

Sample:	DNA dC->dU-editing enzyme APOBEC-3G tetramer, 186 kDa Homo sapiens protein 40-mer single stranded inhibitory DNA dimer, 24 kDa DNA
Buffer:	50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 6

R_g^Guinier	4.7	nm
D_max	16.2	nm
Volume^Porod	395	nm³

SASDM27 – single-stranded DNA dC->dU-editing enzyme APOBEC3G full length in complex with DNA: monomer

40-mer single stranded inhibitory DNADNA dC->dU-editing enzyme APOBEC-3G experimental SAS data

Sample:	40-mer single stranded inhibitory DNA monomer, 12 kDa DNA DNA dC->dU-editing enzyme APOBEC-3G monomer, 46 kDa Homo sapiens protein
Buffer:	50 mM phosphate pH 6.0, 200 mM NaCl, 2 mM β-mercaptoethanol (β-ME), 5% glycerol, 200 µM Na2-EDTA, pH: 6
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 6

R_g^Guinier	3.1	nm
D_max	10.0	nm
Volume^Porod	118	nm³

SASDNM7 – Protease 1 from Pyrococcus horikoshii (PhP1) in Gd-HPDO3A

Sample:	Deglycase PH1704 hexamer, 112 kDa Pyrococcus horikoshii (strain … protein
Buffer:	20 mM Tris pH 7.5, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Jun 7

Medical contrast agents as promising tools for biomacromolecular SAXS experiments. Acta Crystallogr D Struct Biol 78(Pt 9):1120-1130 (2022)
Gabel F, Engilberge S, Schmitt E, Thureau A, Mechulam Y, Pérez J, Girard E

R_g^Guinier	3.2	nm
D_max	9.2	nm
Volume^Porod	140	nm³

SASDNN7 – Protease 1 from Pyrococcus horikoshii (PhP1) in iohexol

Sample:	Deglycase PH1704 hexamer, 112 kDa Pyrococcus horikoshii (strain … protein
Buffer:	20 mM Tris pH 7.5 and 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Nov 26

R_g^Guinier	3.2	nm
D_max	9.2	nm
Volume^Porod	140	nm³

SASDPA4 – SAXS data for the trimeric C-phycocyanin

C-phycocyanin alpha subunitC-phycocyanin beta subunit experimental SAS data

Sample:	C-phycocyanin alpha subunit trimer, 53 kDa Arthrospira platensis protein C-phycocyanin beta subunit trimer, 54 kDa Arthrospira platensis protein
Buffer:	150 mM NaCl, 20 mM Tris, pH: 7.6
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Apr 14

Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores Structural Dynamics 9(5):054701 (2022)
Tsoraev G, Protasova E, Klimanova E, Ryzhykau Y, Kuklin A, Semenov Y, Ge B, Li W, Qin S, Friedrich T, Sluchanko N, Maksimov E

R_g^Guinier	3.9	nm
D_max	13.2	nm
Volume^Porod	154	nm³

SASDPB4 – SAXS data for the C-phycocyanin in 8 M urea solution

Sample:	C-phycocyanin alpha subunit monomer, 18 kDa Arthrospira platensis protein C-phycocyanin beta subunit monomer, 18 kDa Arthrospira platensis protein
Buffer:	8 M urea, 30 mM βME, pH: 7
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Apr 14

R_g^Guinier	6.6	nm
D_max	23.5	nm

SASDMV3 – Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm

Nominal 100 nm diameter polystyrene spheres experimental SAS data

Sample:	Nominal 100 nm diameter polystyrene spheres monomer, 315312 kDa
Buffer:	Water, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 May 16

Standard polystyrene nanospheres (concentration series data)
Dima Molodenskiy

R_g^Guinier	33.0	nm
D_max	100.0	nm
Volume^Porod	520	nm³

SASDMW3 – Thermo Fisher polystyrene nanospheres with a diameter of 20 nm

Nominal 20 nm diameter polystyrene spheres experimental SAS data

Sample:	Nominal 20 nm diameter polystyrene spheres 0, 8 kDa
Buffer:	Water, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 May 16

Standard polystyrene nanospheres (concentration series data)
Dima Molodenskiy

R_g^Guinier	8.5	nm
D_max	25.0	nm
Volume^Porod	3	nm³

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