Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Ab initio only

SASDL52 – Human Heat Shock 70 kDa Protein 1A (Hsp70-1A/HSPA1A)

Heat shock 70 kDa protein 1A experimental SAS data

Sample:	Heat shock 70 kDa protein 1A monomer, 70 kDa Homo sapiens protein
Buffer:	25 mM Tris HCl, 50 mM NaCl, 5 mM Sodium Phosphate, 5 mM KCl, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2018 May 18

Structural, thermodynamic and functional studies of human 71 kDa heat shock cognate protein (HSPA8/hHsc70) Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics :140719 (2021)
Silva N, de Camargo Rodrigues L, Dores-Silva P, Montanari C, Ramos C, Barbosa L, Borges J

R_g^Guinier	3.7	nm
D_max	11.0	nm
Volume^Porod	138	nm³

SASDQ85 – Phosphofructokinase B (PfkB) from Mycobacterium marinum

Fructokinase, PfkB experimental SAS data

Sample:	Fructokinase, PfkB monomer, 32 kDa Mycobacterium marinum (strain … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Dec 17

Structural analysis and functional study of phosphofructokinase B (PfkB) from Mycobacterium marinum Biochemical and Biophysical Research Communications (2021)
Gao B, Ji R, Li Z, Su X, Li H, Sun Y, Ji C, Gan J, Li J

R_g^Guinier	2.0	nm
D_max	6.6	nm
Volume^Porod	58	nm³

SASDL97 – Ru-MtrCAB complex formed by reconstitution of Ru-MtrC and MtrAB

Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrAExtracellular iron oxide respiratory system surface decaheme cytochrome c component MtrCExtracellular iron oxide respiratory system outer membrane component MtrB experimental SAS data

Sample:	Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA monomer, 39 kDa Shewanella oneidensis (strain … protein Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC monomer, 77 kDa Shewanella oneidensis (strain … protein Extracellular iron oxide respiratory system outer membrane component MtrB monomer, 75 kDa Shewanella oneidensis (strain … protein
Buffer:	20 mM HEPES, 100 mM NaCl, 2.8 mM Fos-choline 12, 13% D2O, pH: 7.8
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Jan 13

Bespoke Biomolecular Wires for Transmembrane Electron Transfer: Spontaneous Assembly of a Functionalized Multiheme Electron Conduit Frontiers in Microbiology 12 (2021)
Piper S, Edwards M, van Wonderen J, Casadevall C, Martel A, Jeuken L, Reisner E, Clarke T, Butt J

R_g^Guinier	4.7	nm
D_max	16.6	nm
Volume^Porod	154	nm³

SASDLA7 – Outer membrane protein complex MtrCAB

Sample:	Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA monomer, 39 kDa Shewanella oneidensis (strain … protein Extracellular iron oxide respiratory system outer membrane component MtrB monomer, 75 kDa Shewanella oneidensis (strain … protein Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC monomer, 75 kDa Shewanella oneidensis (strain … protein
Buffer:	20 mM HEPES, 100 mM NaCl, 2.8 mM Fos-choline 12, 13% D2O, pH: 7.8
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Sep 12

R_g^Guinier	5.2	nm
D_max	17.1	nm
Volume^Porod	234	nm³

SASDKL9 – Lymphostatin pH7.5

Sample:	Efa1/LifA protein monomer, 367 kDa Escherichia coli O127:H6 … protein
Buffer:	50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jun 29

Activity of lymphostatin, a lymphocyte inhibitory virulence factor of pathogenic Escherichia coli, is dependent on a cysteine protease motif. J Mol Biol :167200 (2021)
Bease AG, Blackburn EA, Chintoan-Uta C, Webb S, Cassady-Cain RL, Stevens MP

R_g^Guinier	5.9	nm
D_max	19.3	nm
Volume^Porod	559	nm³

SASDKM9 – Lymphostatin pH5.5

Sample:	Efa1/LifA protein monomer, 367 kDa Escherichia coli O127:H6 … protein
Buffer:	50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 5.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jun 29

R_g^Guinier	5.9	nm
D_max	19.7	nm
Volume^Porod	565	nm³

SASDKN9 – Lymphostatin C1480A mutant pH 7.5

Efa1/LifA protein (C1480A mutant) experimental SAS data

Sample:	Efa1/LifA protein (C1480A mutant) monomer, 367 kDa Escherichia coli O127:H6 … protein
Buffer:	50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jun 29

R_g^Guinier	5.9	nm
D_max	19.1	nm
Volume^Porod	557	nm³

SASDKP9 – Lymphostatin C1480A mutant pH5.5

Sample:	Efa1/LifA protein (C1480A mutant) monomer, 367 kDa Escherichia coli O127:H6 … protein
Buffer:	50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 5.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jun 29

R_g^Guinier	5.8	nm
D_max	19.3	nm
Volume^Porod	560	nm³

SASDMM2 – Thalassiosira pseudonana iota Carbonic anhydrase (ι-CA)

iota carbonic anhydrase experimental SAS data

Sample:	Iota carbonic anhydrase tetramer, 260 kDa Thalassiosira pseudonana protein
Buffer:	20 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 28

Structural Contour Map of the Iota Carbonic Anhydrase from the Diatom Thalassiosira pseudonana Using a Multiprong Approach. Int J Mol Sci 22(16) (2021)
Jensen EL, Receveur-Brechot V, Hachemane M, Wils L, Barbier P, Parsiegla G, Gontero B, Launay H

R_g^Guinier	6.7	nm
D_max	25.0	nm
Volume^Porod	500	nm³

SASDMY3 – Lysozyme amyloid fibril (LAF)

lysozyme amyloid fibril experimental SAS data

Sample:	Lysozyme amyloid fibril, 1 kDa Gallus gallus protein
Buffer:	0.2 M glycine-HCl, 80 mM NaCl, pH: 2.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 29

Dependence of the Nanoscale Composite Morphology of Fe3O4 Nanoparticle-Infused Lysozyme Amyloid Fibrils on Timing of Infusion: A Combined SAXS and AFM Study Molecules 26(16):4864 (2021)
Schroer M, Hu P, Tomasovicova N, Batkova M, Zakutanska K, Wu P, Kopcansky P

R_g^Guinier	38.4	nm
D_max	80.0	nm

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